Acta Biotechnol. volume 17, issue 4, P357-363 1997 DOI: 10.1002/abio.370170412 View full text
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J. Poór

Abstract: The cell suspension cultull: Duuclls curotu L. was permenbilized by Tween 80 and immobilized by glutaraldehyde. j3-Galactosidase showed an optimum pH of 4.7 and an optimum temperature of 55 "C. The enzyme hydrolysis was linear for 3 h, reaching a 65% conversion. A very good level of storage stability wils achieved when using dry catalyst, or a solution of 0.15 M NaCl with the addition of chloramphenicol, (I-methyldodecyl)-dimethylamin-4-oxide (ATDNO), chlortetracycline hydrochloride (CLCTC) or by freezing the…

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