Galactosamine decreases nitric oxide formation in cultured rat hepatocytes: Mechanism of suppression

Reactive Nitrogen Species in Acetaminophen-Induced Mitochondrial Damage and Toxicity in Mouse Hepatocytes

“…nNOS has been reported to be present in hepatic mitochondria, which would be consistent with it having a role in mitochondrial toxicity (32, 33); however, its presence in mitochondria has been questioned (34). McMillan utilized immunoblot assays and did not find eNOS in rat hepatocytes (35); however, McNaughton et al reported that eNOS was present in human hepatocytes (36). Thus, the specific NOS isoform important in APAP toxicity will require further investigation.…”

Tumour Necrosis Factor-Alpha and Nitric Oxide Mediate Apoptosis by D-Galactosamine in a Primary Culture of Rat Hepatocytes: Exacerbation of Cell Death by Cocultured Kupffer Cells

“…The studies on iNOS expression in hepatocytes ( Figure 5), and the effect of iNOS inhibition on D-GalN apoptosis ( Table 1), suggested that NO was mediating apoptosis induced by the hepatotoxin in cultured hepatocytes. McMillan (49,50) has found that D-GalN is able to reduce or enhance NO production, whether the hepatotoxin is administered at either 2 h or 10 h after cell isolation, respectively. In concordance with data not included in this study, McMillan (49) has not found any protective effect of iNOS inhibitor on hepatocyte necrosis induced by D-GalN .…”

Glutathione-Dependent Regulation of Nitric Oxide Production in Isolated Rat Hepatocyte Suspensions

“…Hepatocytes were identified as the major source of nitrite production because removal of Kupffer cells after isolation did not significantly lower nitrite production (Nicholls-Grzemski et al, 1999). Recently, the spontaneous production of nitric oxide has also been reported with cultured rat hepatocytes (Lopez-Garcia, 1998;McMillan, 1999).…”