GABA modulation of cholinergic transmission in rat oviduct

Fernández, Inmaculada; Orensanz, Luis M.; Ceballos, Marı́a L. de

doi:10.1016/0024-3205(84)90645-3

Cited by 10 publications

(3 citation statements)

References 17 publications

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“…The moderate activity of GABA-transaminase in muscle layers of the oviduct suggests a possible involvement of the GABAergic system in the modulation of oviducal contractility under physiological conditions. Our morphological observations seem to be in agreement with findings of an effect of GABA on the contractility of isolated rat (Fernandez et al, 1984) and rabbit (Erdö et al, 1984b) oviducts, even though the GABAergic system in this organ is non-neuronal in nature.…”

Section: Discussionsupporting

confidence: 91%

“…Specific GABAA receptor binding sites have been demonstrated in membranes of rat and human oviducts Erd\l=o"\ & Lapis, 1982;Erd\l=o"\et al, 1983). Moreover, contractile responses to GABA have been reported for isolated rabbit (Erdö et al, 1984b) and rat (Fernandez et al, 1984) oviducts. On the basis of the above-mentioned findings, and since surgical denervation of the rat oviduct resulted in a decrease of the tubai GABA content and glutamate decarboxylase activity (Apud et al, 1984), a possible GABAergic innervation of the oviducts was suggested (Apud et al, 1984;Erdö et al, 1984a).…”

Section: Introductionmentioning

confidence: 99%

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Segmental distribution and gestational changes of GABA-transaminase activity in the rat oviduct

Amenta¹,

Cavallotti²,

Mione³

et al. 1986

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The occurrence and the localization of 4-aminobutyrate:2-oxoglutarate transaminase (GABA-transaminase) in the non-pregnant and pregnant rat oviduct were examined using biochemical and enzyme histochemical techniques. Specific GABA-transaminase activity was detected in the ampullary and isthmic portions of the oviduct as well as in the utero-tubal junction. The enzymic activity was lower in the ampullary than in the isthmic or intramural segments of the oviduct. Pregnancy induced a significant increase of GABA-transaminase activity in each portion of the oviduct. Enzyme histochemistry showed the highest GABA-transaminase reactivity at the level of the epithelial cells of the oviduct irrespective of the portion of the tube examined. A faint specific activity was demonstrated in the smooth muscle of the oviduct while the serosa did not show specific staining. Our findings indicate that: the observed increase of GABA-transaminase activity in the oviduct of the pregnant rat may be responsible for the reduced GABA levels in the oviduct during gestation; and the extraneuronal localization of GABA-transaminase activity does not seem to support the suggestion of a possible GABAergic innervation of the oviduct.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Segmental distribution and gestational changes of GABA-transaminase activity in the rat oviduct

Amenta¹,

Cavallotti²,

Mione³

et al. 1986

Reproduction

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“…Since the contraction of the isolated rat oviduct was affected by GABA in vitro (Edro et al, 1984;Fernandez et al, 1984). GABA is thought to act as a modulator of the movement of oviduct muscle.…”

Section: Discussionmentioning

confidence: 99%

Distribution of γ‐Aminobutyric Acid and Glutamate Decarboxylase in the Layers of Rat Oviduct

Murashima

Kato

1986

Journal of Neurochemistry

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An enzymatic microassay method for glutamate decarboxylase (GAD) and gamma-aminobutyric acid (GABA) was improved to yield a high sensitivity and a low blank. The 20-microns thick freeze-dried sections (0.2-1.5 micrograms dry weight) were prepared from the oviduct and ovary of rat. The analysis of these microsamples by the improved method showed that, contrary to the previous observations, the rat ovary is devoid of GAD activity and contains a trace amount of GABA. Both are present abundantly in the oviduct. In the oviduct mucosa, significant GAD activity was found in the estrous phase, whereas the activity was nearly null during other phases of the estrous cycle. GABA concentration in the oviduct mucosa was 10-fold higher than in the cerebral cortex; its variation during the estrous cycle was not remarkable. In the muscle layer of oviduct, GAD activity had a low peak in the estrous phase and GABA concentration was almost constant during the estrous cycle. The denervation experiment showed that GAD is present in the nerve terminals innervating the oviduct.

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