Future Aspects of Bioprocess Monitoring

Abstract

Nature has the impressive ability to efficiently and precisely control biological processes by applying highly evolved principles and using minimal space and relatively simple building blocks. The challenge is to transfer these principles into technically applicable and precise analytical systems that can be used for many applications. This article summarizes some of the new approaches in sensor technology and control strategies for different bioprocesses such as fermentations, biotransformations, and downstream processes. It focuses on bio- and chemosensors, optical sensors, DNA and protein chip technology, software sensors, and modern aspects of data evaluation for improved process monitoring and control.

Optimization of Insect Cell Based Protein Production Processes - Online Monitoring, Expression Systems, Scale Up

“…Bioprocess applications of Raman spectroscopy are feasible since adjustable lasers are available. The technique can be applied for multi-analyte measurements as well as differentiated measurements of certain compounds [61]. Resulting molecular fingerprints are well defined with high chemical specificity even in aqueous systems [62].…”

“…Despite these advantageous, Raman spectroscopy has not been extensively utilized for in situ applications in cell culture processes, which might be related to the difficulties arising with the use of this technique. One of the main problems is the considerable fluorescence activity of several biological molecules that might overlay the Raman scattering bonds [60,61,63]. This is even more a problem when the fluorescent compounds cannot be eliminated, as is the case for culture broths.…”

“…Especially in bioprocess applications, bubbles from aeration and biomass can cause light scattering which results in signal attenuation. In this case, internal referencing can be utilized for correction purposes [61]. The high potential of using Raman spectroscopy in monitoring of cell culture processes was successfully demonstrated by Abu-Absi et al [62].…”

A Flow-Injection System Coupled to a Micro-Guard Cartridge for Monitoring a Vinification Process

“…5 Its scope was further expanded to study chemical and biochemical processes. Due to its versatility and possibility for real-time monitoring, it has also become a powerful tool to study dynamic systems, 6 since it is the proposed subject, the vinification process. These processes are known by their spatial and temporal variability, the chemical composition changes over time.…”

Evaluation of wavelength selection methods for 2D fluorescence spectra applied to bioprocesses characterization

“…On the other hand, the measurement of biological variables such as metabolites, substrate and cellular concentrations demands further improvement (Haack et al, 2004). Several authors have studied different techniques that enable the inference of the variable of interest using chemometric models (Schügerl, 2001;Ferreira et al, 2003;Benoudjita et al, 2004;Becker et al, 2007). Among these, the advantages of 2D fluorescence…”