Acta Biotechnol. volume 18, issue 4, P353-359 1998 DOI: 10.1002/abio.370180410 View full text
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H. Heluane, L. I. C. Defigueroa, F. Vázquez

Abstract: Protoplasts of a xylose-fermenting yeast strain (a fusion product of Pachysolen tannophilus and Saccharomyces cerevisiae) were fused with isolated nuclei of the xylan degrading filamentous fungus Fusarium moniliforme. Polyethyleneglycol 4OOO was used as the fusogenic agent. Fourteen stable hybrids showing xylanase activity were obtained. It can be assumed that this ability was acquired from the nuclear genome of the fungus, since the parental yeast strain did not show any xylanase activity. The enzymatic acti…

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