Further studies on structure of fucoidan from brown alga Saccharina gurjanovae

Structure and anticancer activity in vitro of sulfated galactofucan from brown alga Alaria angusta

“…Probably, Gal residues were terminal to the chains, built up of fucose residues, like it was observed for S. latissima (Bilan et al, 2010). Negative-ion tandem MALDI-TOF spectra of singly-sulfated fucobiose (m/z 389.1) and fucotriose (m/z 535.1) as well as doubly-sulfated fucobiose (m/z 491.0) were typical for (1→3)-linked fucooligosaccharides observed earlier for fucans from S. cichorioides, S. gurjanovae and Costaria costata (Anastyuk et al, 2012a(Anastyuk et al, , 2010Shevchenko et al, 2015), since no intensive signals of cross-ring cleavages of A-or X-type were observed (see Supplementary materials).…”

“…However, in our case Gal residues, probably formed extensive 4-linked chains with branching points at C6, while S. latissima exhibited extensive 6-linked chains with branching points at C4 (Bilan et al, 2010). Shevchenko et al, 2015) proven to be reliable and reproducible method for soft depolymerization of highly sulfated fucans/fucoidans to produce mixtures of low-molecular-weight (LMW) oligosaccharides, carrying structural information. Recently, by using this technique on the galactofucan from Saccharina gurjanovae and the fucan from Saccharina cichorioides (Laminariales) with known (Anastyuk et al, 2010;Zvyagintseva et al, 2003) structure, we have obtained highmolecular-weight (HMW) fractions which were built up of only 3-linked 4-sulfated ␣-L-Fucp residues (Shevchenko et al, 2015).…”

“…Galactofucans from Laminariales, having a backbone of a repeating →3)-␣-L-Fucp-(2,4-SO 3 − )-(1→ residues, required more stressed conditions to detect galactose residues in the oligosaccharide mixtures, since mild conditions of autohydrolysis (25-37 • C) allowed obtaining only short chains, built up of fucose (with DP 1-3, including AaF3LMW fraction, data not shown) along with disaccharides, containing Gal residues mostly on the non-reducing terminus (Anastyuk et al, 2012a;Shevchenko et al, 2015). Probably, Gal residues were terminal to the chains, built up of fucose residues, like it was observed for S. latissima (Bilan et al, 2010).…”

“…Shevchenko et al, 2015) proven to be reliable and reproducible method for soft depolymerization of highly sulfated fucans/fucoidans to produce mixtures of low-molecular-weight (LMW) oligosaccharides, carrying structural information. Recently, by using this technique on the galactofucan from Saccharina gurjanovae and the fucan from Saccharina cichorioides (Laminariales) with known (Anastyuk et al, 2010;Zvyagintseva et al, 2003) structure, we have obtained highmolecular-weight (HMW) fractions which were built up of only 3-linked 4-sulfated ␣-L-Fucp residues (Shevchenko et al, 2015). The structure of the native main chain then was assumed to be represented by a repeating unit →3)-␣-L-Fucp-(2,4-SO 3 − )-(1→ that was shown to be characteristic to Laminariales (Anastyuk et al, 2010;Bilan et al, 2010;Cumashi et al, 2007;Zvyagintseva et al, 2003).…”

Neuroproteomics — LC-MS Quantitative Approaches

“…MS E is perhaps the most used large-screen, label-free method, particularly in the neuroproteomics field, and the only DIA method that has gained enough visibility so far [9,197]. Although MS E was also used in different neuroproteomics areas, such as in the studies of frontotemporal lobar degeneration [198] and the profiling of phosphorylation events in different rat tissues, including the brain [199], its use was particularly potentiated by the Sabine Bahn group for the study of neuropsychiatric diseases, such as schizophrenia, major depression, and bipolar disease [160].…”