Further studies on immunomodulatory effects of exopolysaccharide isolated from Lactobacillus rhamnosus KL37C

Ciszek-Lenda, Marta; Nowak, Bernadeta; Śróttek, Małgorzata; Walczewska, Maria; Górska-Frączek, Sabina; Gamian, Andrzej; Marcinkiewicz, Janusz

doi:10.5114/ceji.2013.37743

Cited by 10 publications

(8 citation statements)

References 24 publications

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“…4 In both probiotic and commensal bacteria, it has been found that many beneficial immunomodulatory phenotypes observed could be attributed to the interaction of bacterial polysaccharides with the host immune system. 5 Additionally, polymers such as lipopolysaccharides and capsular polysaccharides in the form of biofilms are often primary virulence factors associated with different pathogenic bacterial infections. [6][7][8] Extracellular polysaccharides, or exopolysaccharides (EPS) are polymers synthesised during fermentation by a range of bacterial groups, including lactic acid bacteria (LAB) and bifidobacteria, in relatively substantial amounts when compared to cell size.…”

Section: Introductionmentioning

confidence: 99%

Sugar-coated: exopolysaccharide producing lactic acid bacteria for food and human health applications

et al. 2015

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The human enteric microbiome represents a veritable organ relied upon by the host for a range of metabolic and homeostatic functions. Through the production of metabolites such as short chain fatty acids (SCFA), folate, vitamins B and K, lactic acid, bacteriocins, peroxides and exopolysaccharides, the bacteria of the gut microbiome provide nutritional components for colonocytes, liver and muscle cells, competitively exclude potential pathogenic organisms and modulate the hosts immune system. Due to the extensive variation in structure, size and composition, microbial exopolysaccharides represent a useful set of versatile natural ingredients for the food industrial sector, both in terms of their rheological properties and in many cases, their associated health benefits. The exopolysaccharide-producing bacteria that fall within the 35 Lactobacillus and five Bifidobacterium species which have achieved qualified presumption of safety (QPS) and generally recognised as safe (GRAS) status are of particular interest, as their inclusion in food products can avoid considerable scrutiny. In addition, additives commonly utilised by the food industry are becoming unattractive to the consumer, due to the demand for a more 'natural' and 'clean labelled' diet. In situ production of exopolysaccharides by food-grade cultures in many cases confers similar rheological and sensory properties in fermented dairy products, as traditional additives, such as hydrocolloids, collagen and alginate. This review will focus on microbial synthesis of exopolysaccharides, the human health benefits of dietary exopolysaccharides and the technofunctional applications of exopolysaccharide-synthesising microbes in the food industry.

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Section: Introductionmentioning

confidence: 99%

Sugar-coated: exopolysaccharide producing lactic acid bacteria for food and human health applications

et al. 2015

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“…2,3 However, some recent studies have claimed that the immunomodulation of LAB can be achieved not only by living or dead bacteria but also by the components of their cell walls, such as lipoteichoic acid, 4 and secretory products, such as exopolysaccharides (EPS). 5 EPS are long-chain and water-soluble polysaccharides that consist of branched, repeating units of sugars or sugar derivatives. 6 Bacterial EPS are partly secreted into the environment as secondary metabolic products, 5 and partly bound covalently or loosely to the cell surface of microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Immunoregulatory effects on Caco-2 cells and mice of exopolysaccharides isolated from Lactobacillus acidophilus NCFM

Jiang

Yang

et al. 2014

Food Funct.

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On the basis of our previous results on potential immunoregulation of Lactobacillus acidophilus NCFM, the immunoregulatory effects of exopolysaccharides (EPS) isolated from L. acidophilus NCFM and their regulating mechanisms are further investigated in the current research. Stimulated by EPS preparations, four immune-related genes in the human colorectal adenocarcinoma cell line Caco-2 cells, namely, interleukin-1α (IL-1α), chemokine C-C motif 2 (CCL2), tumor necrosis factor α (TNF-α), and pentraxin 3 (PTX3), first showed an increase at 2-4 h, peaked at 4 h, and then decreased at 4-12 h. Similar trends were observed in vivo: four genes showed transient expression (highest on the 4th day) in the cecum and colon of mice. Meanwhile, the organ coefficient, clearance index and phagocytic index all significantly increased with time extension and dose increase of EPS stimulation. EPS triggered NF-κB and p38 mitogen-activated protein kinase (p38 MAPK) signaling pathways in Caco-2 cells, and the activated pathways initiated the genes expression. EPS compounds from L. acidophilus NCFM may play an important role in host immunoregulation and might be applied as a new type of immunoregulatory agent in functional foods.

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“…Also, dissolving the EPS lyophilizate in 0.3 M NaOH followed by centrifugation can help to eliminate extra contaminants (Notararigo et al, 2013). Final purification steps can also consist of ion-exchange chromatography (Behare et al, 2013;Ciszek-Lenda et al, 2013;Górska-Fr czek et al, 2013;Guo et al, 2013;Li et al, 2013Li et al, , 2014aShang et al, 2013;Zhang et al, 2013;Shao et al, 2014;Wang et al, 2014Wang et al, , 2015Fontana et al, 2015), size exclusion chromatography (Górska-Fr czek et al, 2013;Guo et al, 2013;Li et al, , 2014aNotararigo et al, 2013;Shang et al, 2013;Zhang et al, 2013;Miao et al, 2014;Wang et al, 2014Wang et al, , 2015, or preparative SDS-PAGE (Ruas-Madiedo and de los Reyes-Gavilán, 2005). These final purifications are less important when the ultimate aim is to quantify EPS production but more important when the recovered material is used for polysaccharide characterization (Rimada and Abraham, 2003).…”

Section: Methods For Recovery Of Epsmentioning

confidence: 99%

“…Lactic acid bacteria and bifidobacteria are relatively fastidious microorganisms. Hence, complex, nutrient-rich media based on yeast extract and peptones, such as (modifications of) de Man, Rogosa, Sharpe (MRS) medium or tryptone-proteose peptone-yeast extract medium (TPPY), are regularly used to culture EPS-producing LAB, either in liquid broths or on agar (Ciszek-Lenda et al, 2013;Górska-Fr czek et al, 2013;Guo et al, 2013;Li et al, 2013;Zannini et al, 2013;Abdhul et al, 2014;Chen et al, 2014;Lai et al, 2014;Park et al, 2013;Prasanna et al, 2014;Seesuriyachan et al, 2014;Zamfir and Grosu-Tudor, 2014). In the case of bifidobacteria, MRS medium is often supplemented with l-cysteine as an oxygen scavenger (Prasanna et al, 2014).…”

Section: Production Of Epsmentioning

confidence: 99%

“…In addition to their textural benefits, some EPS exert positive effects on consumer health, due to their potential immunomodulatory, antitumor, antiviral, anti-inflammatory, or antioxidant capacities (Prasanna et al, 2014). During the last few years, such aspects have become a hot research topic and have been investigated in several EPS-producing strains of LAB and bifidobacteria (Ciszek-Lenda et al, 2013;Górska-Fr czek et al, 2013;Guo et al, 2013;Marcial et al, 2013;Zhang et al, 2013;Abdhul et al, 2014;Hidalgo-Cantabrana et al, 2014;Lai et al, 2014;Li et al, 2013Li et al, , 2014bShao et al, 2014;Wang et al, 2014Wang et al, , 2015Chen et al, 2015). Both the textural and potential health advantages of EPS are important elements in view of the development of functional starter cultures for the food fermentation industry in general and the dairy industry in particular (Leroy and De Vuyst, 2004;De Vuyst and de Vin, 2007;Ravyts et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Advances in production and simplified methods for recovery and quantification of exopolysaccharides for applications in food and health

Leroy

Vuyst

2016

Journal of Dairy Science

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The capacity of strains to produce exopolysaccharides (EPS) is widespread among species of lactic acid bacteria and bifidobacteria, although the physiological role of these molecules is not yet clearly understood. When EPS are produced during food fermentation, they confer technological benefits on the fermented end products, such as improved texture and stability. In addition, some of these EPS may have beneficial effects on consumer health. These uses of EPS necessitate optimal and sufficient production of these molecules, both in situ and ex situ, not only to improve their yields but also to obtain a particular functionality. The present study reviews the commonly used methods of production, isolation, and quantification that have been used in recent studies dealing with EPS-producing lactic acid bacteria and bifidobacteria.

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Further studies on immunomodulatory effects of exopolysaccharide isolated from Lactobacillus rhamnosus KL37C

Cited by 10 publications

References 24 publications

Sugar-coated: exopolysaccharide producing lactic acid bacteria for food and human health applications

Sugar-coated: exopolysaccharide producing lactic acid bacteria for food and human health applications

Immunoregulatory effects on Caco-2 cells and mice of exopolysaccharides isolated from Lactobacillus acidophilus NCFM

Advances in production and simplified methods for recovery and quantification of exopolysaccharides for applications in food and health

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