Fungus-Bacterium Symbionts Promote Plant Health and Performance

Guo, Yong; Narisawa, Kazuhiko

doi:10.1264/jsme2.me3303rh

Cited by 8 publications

(7 citation statements)

References 54 publications

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“…Much evidence has found that bacterial and fungal mutualism play a major role in promoting and maintaining the host health and performance [32], suggesting that the symbiotic relationship between bacteria and fungi reflects the host's health and disease. Our data showed that the bacterial and fungal abundances in mulberry leaf were significantly different from that in silkworm's intestine.…”

Section: Discussionmentioning

confidence: 99%

The gut microbiota of silkworm are altered by antibiotic exposure

Xiang

et al. 2022

FEBS Open Bio

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In recent years, antibiotics have frequently been detected in soil, lakes, and rivers. Antibiotic residues in the environment may alter microbial structure and cause bacterial resistance, but their effect on interactions among host microbiota is still poorly understood. To investigate this issue, here we used silkworm (Bombyx mori) fed on antibiotic‐treated mulberry leaf as a model to explore the effects of antibiotic exposure on gut bacteria and fungi. We observed that elimination of fungi significantly reduced bacterial richness and diversity in silkworm intestine after exposure to the antifungal amphotericin B, while the elimination of bacteria dramatically increased the richness and diversity of fungi after exposure to the antibacterial ampicillin–streptomycin. Thus, antibiotic‐treated mulberry leaf significantly altered the community structure of microbiota in the gut of silkworm. Clearance of gut bacteria enhanced the correlation between gut fungi and leaf‐derived fungi, while clearance of gut fungi promoted abnormal proliferation of gut bacteria. These data provide a simple model to explore the comprehensive effect of diet‐derived bacteria, fungi, and antibiotics on gut microbiota.

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Section: Discussionmentioning

confidence: 99%

The gut microbiota of silkworm are altered by antibiotic exposure

Xiang

et al. 2022

FEBS Open Bio

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“…In addition to the NACHT-based architectures present in the cluster, the web-based profile HMM search revealed several additional homologs in NLRs with the NB-ARC TPR domains. Interestingly, homologous domains are also present as separate proteins in Mucormycota Rhizophagus irregularis , a species related to Mortierella , and in association with NACHT WD40 and NACHT HEAT in Mycoavidus cysteinexigens , in accordance with the possibility of horizontal gene transfer [81]. A good quality structural model supports homology to TIR and HET domains (Fig.…”

Section: Resultsmentioning

confidence: 82%

Exploring a diverse world of effector domains and amyloid signaling motifs in fungal NLR proteins

Wojciechowski

Tekoglu

Gąsior-Głogowska

et al. 2022

Preprint

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NLR proteins are intracellular receptors constituting a conserved component of the innate immune system of multicellular organisms. In fungi, NLRs are characterized by high diversity of architectures and presence of amyloid signaling. Here, we explore the diverse world of effector and signaling domains of fungal NLRs using state-of-the-art bioinformatic methods including MMseqs2 for fast clustering, probabilistic context-free grammars for sequence analysis, and AlphaFold2 deep neural networks for structure prediction. In addition to substantially improving the overall annotation, especially in basidiomycetes, the study identifies novel domains and reveals the structural similarity of MLKL-related HeLo- and Goodbye-like domains forming the most abundant superfamily of fungal NLR effectors. Moreover, compared to previous studies, we found several times more amyloid motifs, including novel families, and validated aggregating and prion-forming properties of the most abundant of them in vitro and in vivo. Also, through an extensive in silico search, the NLR-associated amyloid signaling is for the first time identified in basidiomycetes. The emerging picture highlights similarities and differences in the NLR architectures and amyloid signaling in ascomycetes, basidiomycetes and other branches of life.

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“…Importantly, the TIR domain was reported in NLRs from plants, bacteria and Chytridiomycota [ 5 , 12 , 21 , 86 ]. Interestingly, homologous domains were also present as separate proteins in Mucormycota Rhizophagus irregularis , a species related to Mortierella , and in Mycoavidus cysteinexigens , in accordance with the possibility of horizontal gene transfer [ 87 ].…”

Section: Resultsmentioning

confidence: 99%

Exploring a diverse world of effector domains and amyloid signaling motifs in fungal NLR proteins

et al. 2022

View full text Add to dashboard Cite

NLR proteins are intracellular receptors constituting a conserved component of the innate immune system of cellular organisms. In fungi, NLRs are characterized by high diversity of architectures and presence of amyloid signaling. Here, we explore the diverse world of effector and signaling domains of fungal NLRs using state-of-the-art bioinformatic methods including MMseqs2 for fast clustering, probabilistic context-free grammars for sequence analysis, and AlphaFold2 deep neural networks for structure prediction. In addition to substantially improving the overall annotation, especially in basidiomycetes, the study identifies novel domains and reveals the structural similarity of MLKL-related HeLo- and Goodbye-like domains forming the most abundant superfamily of fungal NLR effectors. Moreover, compared to previous studies, we found several times more amyloid motif instances, including novel families, and validated aggregating and prion-forming properties of the most abundant of them in vitro and in vivo. Also, through an extensive in silico search, the NLR-associated amyloid signaling was identified in basidiomycetes. The emerging picture highlights similarities and differences in the NLR architectures and amyloid signaling in ascomycetes, basidiomycetes and other branches of life.

show abstract

Fungus-Bacterium Symbionts Promote Plant Health and Performance

Cited by 8 publications

References 54 publications

The gut microbiota of silkworm are altered by antibiotic exposure

The gut microbiota of silkworm are altered by antibiotic exposure

Exploring a diverse world of effector domains and amyloid signaling motifs in fungal NLR proteins

Exploring a diverse world of effector domains and amyloid signaling motifs in fungal NLR proteins

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