Fungistatic activity of<i>Zanthoxylum rhoifolium</i>Lam. bark extracts against fungal plant pathogens and investigation on mechanism of action in<i>Botrytis cinerea</i>

Carotenuto, Gennaro; Carrieri, Raffaele; Tarantino, Piero; Alfieri, Mariaevelina; Leone, Antonietta; Tommasi, Nunziatina De; Lahoz, E.

doi:10.1080/14786419.2014.1000894

Cited by 7 publications

(7 citation statements)

References 16 publications

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“…According to Carotenuto et al (2015) with modifications, the evaluation of inhibition of mycelial growth of the phytopathogen was carried out by adding to the surface of the 9 cm diameter Petri dish containing approximately 20 ml of BDA culture medium already solidified, 100 μL of the extracts of the endophytic fungi. The extracts were diluted in Tween 80 and water.…”

Section: Selection According To Fungitoxicity Against C Lunatamentioning

confidence: 99%

Secondary metabolites from endophytic fungus from Lippia sidoides Cham.

Ferreira¹,

Santos²,

Soares³

et al. 2017

J. Med. Plants Res.

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Lippia sidoidesCham. (Verbenaceae) is a species native to the Brazilian northeast, widely used in popular medicine. Its leaves were used for the isolation of endophytic fungi and extraction of metabolites. Among them, three were selected according to fungitoxicity tests against the maize phytopathogenic fungus, Curvularia lunata (Wakker). However, the objective of this study was to identify the role of L. sidoides extracts associated with their endophytic fungi, necessary to reduce excess of fungicides applied on the maize crop. Metabolites were evaluated for antioxidant activity by 2.2-diphenyl-1-picrylhydrazyl (DPPH), phenols, total flavonoids and one of it endophytic fungus were evaluated for synergism (Verticilium sp. and plant extracts). The endophytic fungi and plant extracts evaluated for phenolic content ranged from 0.29 ± 0.05 to 96.94 ± 11.86 mgEAG/g, the content of flavonoids from 14.31 ± 1.56 to 192.33 ± 4.58 mgER/g, and antioxidant activity could only be observed for the plant extract with EC 50 81 ± 0.3%. The secondary metabolites identified by HPLC in the plant extract were catechin, quercetin, gallic acid and naringin. Naringenin, catechin, epigallocatechin gallate and quercetin were identified in the extract of the fungi viz. Verticillium sp. and Fusarium sp. Synergistic analysis between a 1:1 proportion of plant and fungal extracts has shown more efficient (79.0%) inhibition of C. lunata. Thus, alternative control of phytopathogenic fungi can be accomplished using plant extracts associated with their endophytic fungi, reducing the excess of fungicides applied on the maize crop.

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Section: Selection According To Fungitoxicity Against C Lunatamentioning

confidence: 99%

Secondary metabolites from endophytic fungus from Lippia sidoides Cham.

Ferreira¹,

Santos²,

Soares³

et al. 2017

J. Med. Plants Res.

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“…Costa et al (2008) identified antibacterial and larvicidal effects in the essential oil extracted from the fruit. Z. rhoifolium bark extract showed analgesic activity, popularly mentioned for toothache and earache, as well as fungicidal activity (Pereira, et al, 2010;Carotenuto, et al, 2015). The study by Jullian et al (2006) was able to prove the antimalarial potential of the shell.…”

Section: Introductionmentioning

confidence: 86%

“…The bark extract has also demonstrated analgesic activity, popularly mentioned for toothache and earache, as well as fungicidal activity (Pereira, et al, 2010;Carotenuto, et al, 2015). Furthermore, Jullian et al (2006) were able to prove the antimalarial potential of the shell.…”

Section: Introductionmentioning

confidence: 94%

Vasorelaxant activity and acute toxicity of the ethanolic extract of Zanthoxylum rhoifolium Lam leaves

Silva

Azevedo

Souza

et al. 2019

Braz. J. Pharm. Sci.

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The study evaluated the vasorelaxant effect induced by the ethanolic extract of the leaves of Zanthoxylum rhoifolium Lam (EEtOH-Zr/leaves). Wistar rats were treated with the leaf extract containing a single dose of 2,000 mg / kg, v.o. After 14 days, the animals were anesthetized for blood collection and subsequent analysis of the biochemical parameters; they were then euthanized (sodium pentobarbital-100 mg/kg, i.p.) for the removal and morphological analysis of the heart, lung, liver and kidney. The vasorelaxation activity the and vascular reactivity of EEtOH-Zr/leaves were evaluated on artery mesenteric rings isolated from rats. The extract showed no signs of toxicity and no significant difference in the values of the biochemical parameters between the control group and the group of treated animals. In the evaluation of pharmacological activity in the smooth muscle, the EEtOH-Zr/leaves caused vasorelaxant effect on the tonic contraction induced by phenylephrine in mesenteric artery preparations in the presence (pD2=2.17±0.05 µg/mL; Emax=99.8±5.2%) and absence (pD2=2.14±0.05 µg/mL; Emax=95.3±6.4%) of the vascular endothelium. Oral administration of EEtOH-Zr/leaves reduced the contraction induced by the cumulative addition of PHE. It is concluded that the EEtOH-Zr/leaves promote vasorelaxation and reduce vascular reactivity of adrenergic alpha-1 agonist in the mesenteric artery. The results did not show toxic effects of the extract.

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“…The genus Zanthoxylum (Rutaceae) includes about 250 species distributed throughout the world; among these, the Zanthoxylum rhoifolium species, native to South America, has long been used in traditional Brazilian medicine to treat various health problems [34] and in French Guiana as antimalarial treatment [35]. These plants are known to be a rich source of natural alkaloids and lignans, which are extracted from the bark [36]. Some of these alkaloids, including benzofenantridine and fluoroquinolones, exhibit a variety of biological activities: anticancer [37], antimicrobial [38], anti-inflammatory [39] and antifungal [40,41] activities.…”

Section: Introductionmentioning

confidence: 99%

“…Some of these alkaloids, including benzofenantridine and fluoroquinolones, exhibit a variety of biological activities: anticancer [37], antimicrobial [38], anti-inflammatory [39] and antifungal [40,41] activities. Moreover, recently, Zanthoxylum rhoifolium extracts showed a fungistatic effect on different fungal plant pathogens, such as, Botrytis cinerea, Sclerotinia sclerotiorum, Alternaria alternata, Colletotrichum gloeosporioides and Clonostachys rosea [36].…”

Section: Introductionmentioning

confidence: 99%

Growth, Survival and Spore Formation of the Pathogenic Aquatic Oomycete Aphanomyces astaci and Fungus Fusarium avenaceum Are Inhibited by Zanthoxylum rhoifolium Bark Extracts In Vitro

Pagliarulo

Sateriale

Scioscia

et al. 2018

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This study aimed to evaluate the in vitro activity of Zanthoxylum rhoifolium bark (Zr-b) extracts against pathogenic aquatic oomycete/fungal isolates that cause different diseases in native European crayfish resulting in an elevated mortality rate and severe economic repercussions. n-hexane, chloroform, chloroform-methanol (9:1) and methanol extracts of Zr-b were used to evaluate the antifungal activity against the strain UEF88662 of Aphanomyces astaci (oomycete) and the strain SMM2 of Fusarium avenaceum (fungus). The anti-oomycete and antifungal activity was quantitatively evaluated by growth, survival and sporulation microbiological assays. The extracts tested demonstrated a dose-dependent inhibitory effect on oomycete and fungal growth and survival, as well as on the production of oomycete and fungal spores. This work presents alternatives for the treatment and prevention of the spreading of Aphanomyces astaci and Fusarium avenaceum, the etiological agents of the diseases crayfish plague and brown spot disease, respectively. The antifungal properties of Zanthoxylum rhoifolium bark extracts warrant further research on their use in the prevention and treatment of both oomycete and fungal diseases. The antifungal properties of Zanthoxylum rhoifolium bark extracts, shown in vitro, indicate the possibility of their use in new therapeutic and prophylactic strategies, providing perspectives for the design of in vivo studies.

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Fungistatic activity ofZanthoxylum rhoifoliumLam. bark extracts against fungal plant pathogens and investigation on mechanism of action inBotrytis cinerea

Cited by 7 publications

References 16 publications

Secondary metabolites from endophytic fungus from Lippia sidoides Cham.

Secondary metabolites from endophytic fungus from Lippia sidoides Cham.

Vasorelaxant activity and acute toxicity of the ethanolic extract of Zanthoxylum rhoifolium Lam leaves

Growth, Survival and Spore Formation of the Pathogenic Aquatic Oomycete Aphanomyces astaci and Fungus Fusarium avenaceum Are Inhibited by Zanthoxylum rhoifolium Bark Extracts In Vitro

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