Fungicide Resistance in Fusarium graminearum Species Complex

Chaves, Magda Antunes de; Reginatto, Paula; Costa, Bárbara Souza da; Paschoal, Ricardo Itiki de; Teixeira, Mário Lettieri; Fuentefría, Alexandre Meneghello

doi:10.1007/s00284-021-02759-4

Cited by 44 publications

(43 citation statements)

References 71 publications

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“…In recent years, food safety has become an important topic, and synthetic fungicides have been shown to cause serious health problems in humans [38][39][40]. Moreover, F. graminearum has developed resistance to synthetic fungicides, such as azoles and carbendazim, because of their long-term usage [41][42][43][44][45]. Therefore, it is of great significance to develop active ingredients of biological fungicides to combat this critical pathogen.…”

Section: Discussionmentioning

confidence: 99%

Identification of the Causal Agent of Brown Leaf Spot on Kiwifruit and Its Sensitivity to Different Active Ingredients of Biological Fungicides

et al. 2022

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Kiwifruit (Actinidia chinensis) is an important commercial crop in China, and the occurrence of diseases may cause significant economic loss in its production. In the present study, a new pathogen that causes brown leaf spot disease on kiwifruit was reported. The fungus was isolated from an infected sample and identified as Fusarium graminearum based on morphological and molecular evaluation. Koch’s postulates were confirmed when the pathogen was re-isolated from plants with artificially induced symptoms and identified as F. graminearum. Based on the biological characteristics of the pathogen, it was determined that: its optimal growth temperature was 25 °C; optimal pH was 7; most suitable carbon source was soluble starch; most suitable nitrogen source was yeast powder; and best photoperiod was 12 h light/12 h dark. Further investigations were conducted by determining 50% effective concentrations (EC50) of several active ingredients of biological fungicides against F. graminearum. The results showed that among the studied fungicides, tetramycin and honokiol had the highest antifungal activity against this pathogen. Our findings provide a scientific basis for the prevention and treatment of brown leaf spot disease on kiwifruit.

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Section: Discussionmentioning

confidence: 99%

Identification of the Causal Agent of Brown Leaf Spot on Kiwifruit and Its Sensitivity to Different Active Ingredients of Biological Fungicides

et al. 2022

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“…However, the applicability of using the cytochrome bc1 inhibitor and phenazines together to improve their efficacy was proven to be effective on the strobilurin-sensitive fungus Fusarium graminearum (Figure S5). It was indicated that the efficacy of Xanthomonas pathogens can be hopefully improved by the combined use of the effective cytochrome bc1 inhibitors and phenazines.…”

Section: Discussionmentioning

confidence: 99%

Cytochrome bc1 Complex: Potential Breach to Improve the Activity of Phenazines on Xanthomonas

Teng

Mao

et al. 2022

J. Agric. Food Chem.

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The effects of the natural pesticides, phenazines, were reported to be limited by some tolerant metabolism processes within Xanthomonas. Our previous studies suggested that the functional cytochrome bc1 complex, the indispensable component of the respiration chain, might participate in tolerating phenazines in Xanthomonas. In this study, the cytochrome bc1 mutants of Xanthomonas campestris pv. campestris (Xcc) and Xanthomonas oryzae pv. oryzae (Xoo), which exhibit different tolerance abilities to phenazines, were constructed, and the cytochrome bc1 complex was proven to partake a critical and conserved role in tolerating phenazines in Xanthomonas. In addition, results of the cytochrome c mutants suggested the different functions of the various cytochrome c proteins in Xanthomonas and that the electron channeled by the cytochrome bc1 complex to cytochrome C4 is the key to reveal the tolerance mechanism. In conclusion, the study of the cytochrome bc1 complex provides a potential strategy to improve the activity of phenazines against Xanthomonas.

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“…17 Considering that resistance to triazoles and benzimidazoles recommended combating F. graminearum has been reported, it is of great importance to develop environmentally friendly alternative approaches for FHB management. 18 Trans-2-hexenal (T2H, C 6 H 10 O) is a typical green leaf volatile synthesized from linolenic acid or linoleic acid through the LOX pathway. 3 As a lipophilic molecule, T2H has a lower density (0.849 g/mL) than water and is insoluble in water (https://pubchem.ncbi.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Considering that resistance to triazoles and benzimidazoles recommended combating F. graminearum has been reported, it is of great importance to develop environmentally friendly alternative approaches for FHB management .…”

Section: Introductionmentioning

confidence: 99%

Green Leaf Volatile Trans-2-Hexenal Inhibits the Growth of Fusarium graminearum by Inducing Membrane Damage, ROS Accumulation, and Cell Dysfunction

Wang

Zhu

et al. 2022

J. Agric. Food Chem.

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Fusarium graminearum, the main agent of Fusarium head blight (FHB), can cause serious yield loss and secrete mycotoxins to contaminate grain. Here, the biological activity of trans-2-hexenal (T2H) against F. graminearum was determined and its mode of action (MOA) was investigated. Furthermore, surface plasmon resonance with liquid chromatography–tandem mass spectrometry (SPR-LC–MS/MS), bioinformatic analysis, and gene knockout technique were combined to identify the binding proteins of T2H in F. graminearum cells. T2H exhibited satisfactory inhibitory activity against F. graminearum in vitro. Good lipophilicity greatly enhanced the affinity of T2H to F. graminearum mycelia and further caused membrane damage. The FgTRR (thioredoxin reductase) gene negatively regulates the sensitivity of F. graminearum to T2H by reducing the generation of reactive oxygen species (ROS) induced by T2H. Two mutant strains with FgSLX1 (structure-specific endonuclease subunit) and FgCOPB (coatomer subunit β) genes knockout showed decreased sensitivity to T2H, suggesting that these two genes may be involved in the antimicrobial activity of T2H. Taken together, T2H can inhibit F. graminearum growth by multiple MOAs and can be used as a biofumigant to control the occurrence of FHB in the field.

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Fungicide Resistance in Fusarium graminearum Species Complex

Cited by 44 publications

References 71 publications

Identification of the Causal Agent of Brown Leaf Spot on Kiwifruit and Its Sensitivity to Different Active Ingredients of Biological Fungicides

Identification of the Causal Agent of Brown Leaf Spot on Kiwifruit and Its Sensitivity to Different Active Ingredients of Biological Fungicides

Cytochrome bc1 Complex: Potential Breach to Improve the Activity of Phenazines on Xanthomonas

Green Leaf Volatile Trans-2-Hexenal Inhibits the Growth of Fusarium graminearum by Inducing Membrane Damage, ROS Accumulation, and Cell Dysfunction

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