Fungal Jasmonate as a Novel Morphogenetic Signal for Pathogenesis

Liu, Yingyao; Pagac, Martin; Yang, Fan; Patkar, Rajesh; Naqvi, Naweed I.

doi:10.3390/jof7090693

Cited by 8 publications

(10 citation statements)

References 53 publications

(72 reference statements)

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“…Likewise, two JA knock-down mutants, AOCRi and OPRRi , deficient in JA accumulation, develop disease symptoms to the same extent as wild type plants during both, compatible and incompatible, interactions, suggesting that JA is dispensable for the defence against M. oryzae [ 32 ]. Even worse, a recent finding that M. oryzae produces jasmonates by itself to promote appressorium formation challenges the role of JA as promoting factor against M. oryzae [ 33 ].…”

Section: Introductionmentioning

confidence: 99%

Jasmonic acid contributes to rice resistance against Magnaporthe oryzae

Morel

Riemann

et al. 2022

BMC Plant Biol

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Background The annual yield losses caused by the Rice Blast Fungus, Magnaporthe oryzae, range to the equivalent for feeding 60 million people. To ward off infection by this fungus, rice has evolved a generic basal immunity (so called compatible interaction), which acts in concert with strain-specific defence (so-called incompatible interaction). The plant-defence hormone jasmonic acid (JA) promotes the resistance to M. oryzae, but the underlying mechanisms remain elusive. To get more insight into this open question, we employ the JA-deficient mutants, cpm2 and hebiba, and dissect the JA-dependent defence signalling in rice for both, compatible and incompatible interactions. Results We observe that both JA-deficient mutants are more susceptible to M. oryzae as compared to their wild-type background, which holds true for both types of interactions as verified by cytological staining. Secondly, we observe that transcripts for JA biosynthesis (OsAOS2 and OsOPR7), JA signalling (OsJAZ8, OsJAZ9, OsJAZ11 and OsJAZ13), JA-dependent phytoalexin synthesis (OsNOMT), and JA-regulated defence-related genes, such as OsBBTI2 and OsPR1a, accumulate after fungal infection in a pattern that correlates with the amplitude of resistance. Thirdly, induction of defence transcripts is weaker during compatible interaction. Conclusion The study demonstrates the pivotal role of JA in basal immunity of rice in the resistance to M. oryzae in both, compatible and incompatible interactions.

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Section: Introductionmentioning

confidence: 99%

Jasmonic acid contributes to rice resistance against Magnaporthe oryzae

Morel

Riemann

et al. 2022

BMC Plant Biol

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“…The only well-studied microbial jasmonate remains coronatine from the biotrophic pathogenic bacterium Pseudomonas syringae , which, upon entering the host plant tissues, activates JA signaling and suppresses SA-based plant defense [ 55 , 56 ]. Jasmonates were found in several fungal species, such as Magnaporthe oryzae , Lasiodiplodia theobromae , Fusarium oxysporum , and Gibberella fujikuroi [ 57 , 58 , 59 , 60 ]. Particularly important is the fact that JA and its derivate, 12-hydroxyjasmonic acid, are secreted by the fungus into plant tissue to suppress the JA-dependent host immunity [ 57 ], to stop the vegetative phase, and to induce pathogenic development [ 60 ].…”

Section: Discussionmentioning

confidence: 99%

12-Oxophytodienoate Reductase Overexpression Compromises Tolerance to Botrytis cinerea in Hexaploid and Tetraploid Wheat

Degtyaryov,

Pigolev,

Miroshnichenko

et al. 2023

Plants

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12-Oxophytodienoate reductase is the enzyme involved in the biosynthesis of phytohormone jasmonates, which are considered to be the major regulators of plant tolerance to biotic challenges, especially necrotrophic pathogens. However, we observe compromised tolerance to the necrotrophic fungal pathogen Botrytis cinerea in transgenic hexaploid bread wheat and tetraploid emmer wheat plants overexpressing 12-OXOPHYTODIENOATE REDUCTASE-3 gene from Arabidopsis thaliana, while in Arabidopsis plants themselves, endogenously produced and exogenously applied jasmonates exert a strong protective effect against B. cinerea. Exogenous application of methyl jasmonate on hexaploid and tetraploid wheat leaves suppresses tolerance to B. cinerea and induces the formation of chlorotic damages. Exogenous treatment with methyl jasmonate in concentrations of 100 µM and higher causes leaf yellowing even in the absence of the pathogen, in agreement with findings on the role of jasmonates in the regulation of leaf senescence. Thereby, the present study demonstrates the negative role of the jasmonate system in hexaploid and tetraploid wheat tolerance to B. cinerea and reveals previously unknown jasmonate-mediated responses.

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“…retention times and MRM transitions, for profiling of 214 lipid mediators, including 18 deuterium-labelled standards. LC-MS/MS parameters for detection of jasmonic acid were applied as described previously (31). Peak identification and integration was performed using the LabSolutions workstation software.…”

Section: Methodsmentioning

confidence: 99%

“…The method contains analytical conditions, i.e. retention times and MRM transitions, for profiling of parameters for detection of jasmonic acid were applied as described previously (31).…”

Section: Analysis Of Lipid Mediators Using Targeted Lc-ms/msmentioning

confidence: 99%

Life stage impact on the human skin ecosystem: lipids and the microbial community

Pagac,

Davient,

Lam

et al. 2024

Preprint

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While research into gut-microbe interactions is common and advanced, with multiple defined impacts on human health, studies exploring the significance of skin-microbe interactions remain underrepresented. Skin is the largest human organ, has a vast surface area, and is inhabited by a plethora of microorganisms which metabolise sebaceous lipids. Sebaceous free fatty acids are metabolized into bioactive lipid mediators with immune-modulatory properties by skin-resident microbes, including Malassezia. Intriguingly, many of the same lipid mediators are also found on human skin, implying these compounds may have microbial or mixed microbial/human origin. To support this hypothesis, we isolated lipids and microbial DNA from the skin of prepubescent, adult, pre- and post-menopausal volunteers and performed correlational analyses using skin lipidomics and metagenomics to compare lipid mediator profiles and microbiome compositions on skin with either low or high sebaceous gland activity. We found that specific microbial taxonomies were positively and negatively correlated with skin lipid mediator species with high statistical significance. 2D in vitro co-cultures with Malassezia and keratinocytes also directly linked the production of specific lipid mediators, detected on healthy human skin, to secretion of immuno-stimulatory cytokines. Together, these findings further support the hypothesis that microbial-derived skin lipid mediators influence healthy skin homeostasis and skin disease development and progression, thereby spotlighting the relevance of the skin microbiome footprint on human health.

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Fungal Jasmonate as a Novel Morphogenetic Signal for Pathogenesis

Cited by 8 publications

References 53 publications

Jasmonic acid contributes to rice resistance against Magnaporthe oryzae

Jasmonic acid contributes to rice resistance against Magnaporthe oryzae

12-Oxophytodienoate Reductase Overexpression Compromises Tolerance to Botrytis cinerea in Hexaploid and Tetraploid Wheat

Life stage impact on the human skin ecosystem: lipids and the microbial community

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