Fungal isolates from marine sponge Chelonaplysilla sp.: Diversity, antimicrobial and cytotoxic activities

Abstract

Abstract. Handayani D, Artasasta MA, Safira N, Ayuni DF, Tallei TE, Hertiani T. 2020. Fungal isolates from marine sponge Chelonaplysilla sp.: Diversity, antimicrobial and cytotoxic activities. Biodiversitas 21: 1954-1960. The purpose of this research was to study the diversity of fungi associated with marine sponges Chelonaplysilla sp. and their bioactivities. Fungal isolation was carried out by the multilevel dilution method in Saboraud Dextrose Agar (SDA). Twelve fungal isolates were successfully purified, then cultivated using rice for 4-6 weeks at room temperature and subsequently extracted using ethyl acetate. Antimicrobial activities of the fungal extracts were tested against Staphylococcus aureus, Escherichia coli, and Candida albicans by using the agar diffusion method. The extracts of isolates Ch05 and Ch12 showed a significant antagonistic effect against S. aureus and E. coli with the diameter that ranged from 15 to 17 mm. Using the brine shrimp lethality test (BSLT), six fungal extracts revealed cytotoxic activity with LC50 <100 µg/mL. Isolate Ch10 was the most potential fungus with the strong cytotoxic activity of LC50 of 0.90 µg/mL. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was conducted also for six potential fungal extracts against breast cancer cell (T47D). The isolate Ch05 showed moderate cytotoxic activity with IC50 of 83.69 µg/mL. The molecular identification was carried out for potential fungi using the ITS marker. The results showed that Ch02 was Aspergillus oryzae, Ch05 was Phomompsis sp., Ch06 was Penicillium simplicissimum, Ch10 was B. bassiana and Ch12 was Aspergillus mellinus. This study concluded that fungal isolates from marine sponge Chelonaplysilla sp. can be explored further for new sources of antimicrobial and anticancer compounds.

Antitumor immunity and therapeutic properties of marine seaweeds-derived extracts in the treatment of cancer

“…Seaweeds-derived products and their derivatives possess antitumor properties via modulating immune mechanism and a variety of cellular and molecular mechanisms, inducing apoptosis, cell cycle progression blocking, modulating transduction signal pathways, preventing cancer cell invasion, and metastasis, stopping angiogenesis and antioxidant activity [65][66][67][68][69][70][71].…”

The Cytotoxic Activity of Marine Sponge-Derived Fungus Aspergillus nomius NC06 Against HT29 Colon Cancer Cells

“…The specimens were carefully cut to obtain 10-12 cm samples. Subsequently, the samples underwent immediate sterilization using 95% ethanol and were further processed in the laboratory [8].…”

“…The absorbance of the reaction with the addition of MTT reagent was measured using a Tecan Microplate reader at a maximum wavelength of 560 nm. The absorbance of each fraction was converted into percent cell viability, which was then used to generate a regression curve to determine the IC50 [7,8,9].…”

AKTIVITAS ANTIBAKTERI DARI FUNGI ENDOFIT Caulerpa racemosa ASAL PERAIRAN PULAU LEMUKUTAN (ANTIBACTERIAL ACTIVITY FROM ENDOPHYTIC FUNGI Caulerpa racemosa ORIGIN IN LEMUKUTAN ISLAN WATERS)

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