Fungal-derived extracts induce resistance against Botrytis cinerea in Arabidopsis thaliana

Hael-Conrad, V.; Grau, Rodrigo Hernán Tomas; Moschen, Sebastián; Serra, Fernanda; Ricci, Juan C. Díaz; Salazar, Sergio Miguel

doi:10.1007/s10658-020-02054-1

Cited by 5 publications

(11 citation statements)

References 52 publications

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“…The JA-mediated response also reported under colonization by Clonostachys rosea is capable of systemically increasing PAL and PPO activity [105]. However, for Colletotrichum acutatum and C. fragariae, this is an SA-mediated response, causing a systemic increase in PR-1 expression, hydrogen peroxide accumulation, and callose deposition in A. thaliana and strawberry plants [106,108]. In addition, as verified in chickpea plants with the endophyte Piriformospora indica, greater control of the disease is directly related to greater root colonization [114].…”

Section: Fungi As Inductors Of Plant Resistance Against B Cinereamentioning

confidence: 89%

Insight into the Microbiological Control Strategies against Botrytis cinerea Using Systemic Plant Resistance Activation

2020

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Botrytis cinerea is a polyphagous necrotrophic fungus and is the causal agent of grey mold diseases in more than 1400 different hosts. This fungus causes serious economic losses in both preharvest and post-harvest—mainly in grape, strawberry, and tomato crops—and is the second most important pathogen worldwide, to our knowledge. Beneficial bacteria and fungi are efficient biocontrol agents against B. cinerea through direct mechanisms, such as parasitism, antibiosis, and competition, but also indirectly through the activation of systemic plant resistance. The interaction between plants and these microorganisms can lead to the development of defensive responses in distant plant organs, which are highly effective against foliar, flower, and fruit pathogens, such as B. cinerea. This review aimed to explore the systemic plant defense responses against B. cinerea by compiling all cases reported (to the best of our knowledge) on the use of beneficial bacteria and fungi for agriculture, a subject not yet specifically addressed.

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Section: Fungi As Inductors Of Plant Resistance Against B Cinereamentioning

confidence: 89%

Insight into the Microbiological Control Strategies against Botrytis cinerea Using Systemic Plant Resistance Activation

2020

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“…Two elicitors were obtained from strawberry leaves, the Fragarin [24] and the Ellagitanin HeT [25] and the elicitor Acremonium strictum Elicitor Subtilisin (AsES) that was purified from the avirulent fungus Acremonium strictum [26][27][28][29][30][31][32] . We have also investigated the two synthetic brassinosteroids (e.g., EP24 and BB16) with both plant defense and plant growth stimulation capacities [33][34][35] , and fungal-derived extracts, which are a complex mixture of elicitors-the latter may be comparable those vaccines made with whole inactivated pathogens [36,37] . Just to mention, we have also induced innate defenses in strawberry plants through a soft mechanical stimulus [38] , similar to what was previously observed by Benikhlef et al [39] in the model plant Arabidopsis thaliana.…”

Section: Immunization Agents and Types Of (Phyto) Vaccinesmentioning

confidence: 99%

Phytovaccines, a New Paradigm for Crop Protection: Towards a Sustainable Agriculture

Tomas-Grau,

Martos,

Castagnaro

et al. 2022

J Mod Agric Biotechnol

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In this comment, we propose to incorporate the concepts "phytovaccines" to refer to bioproducts containing defense elicitors, and "phytovaccination" to the process of applying such phytovaccines to activate plants' innate immune system. We bring support to these concepts based on the traditional process of human vaccination. We posit that plant defense elicitors are to plants what antigens are for humans, and that the elicitor-induced innate immune system in plants is to some extent similar to the immunization process triggered by an antigen in humans-saving the differences between both kinds of organisms. Both defense responses share many features, including an immunization agent (elicitor/antigen), the recognition of such agent by a receptor that initiates a cascade of reactions tending to activate a systemic response, that has a long-term effect and can passively be transmitted across the placenta to newborn infants in the case of humans, and to following generations of plants agamically propagated. We further compare phytovaccination to transgenic plants, as two valid biotechnological tools for crop protection, highlighting the benefits of the former. We encourage the academic and agricultural community in general and the plant-pathogen interaction community in particular, to incorporate the use of phytovaccines and phytovaccination into the biocontrol vocabulary.

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“…The fungal-derived extract CF was prepared from the culture of the isolate M11 of Colletotrichum acutatum, according to the method described by Hael Conrad et 3/12 https://www.doi.org/10.53964/jmab.2022025 al. [38] , 2020. The fungus was gently provided by the fungi collection from the plant biotechnology laboratory at Universidad Nacional de Tucumán, Argentina.…”

Section: Cf Extract Obtentionmentioning

confidence: 99%

“…Briefly, a liquid static culture of M11 was grown in potato dextrose broth (PDB) at 28°C under continuous fluorescent light (300μmol/m 2 s). After ten days, it was gauze-filtrated and the liquid fermented culture constituted the extract CF, which was autoclaved (121°C, 1atm, 20min) to inactivate conidia and cell debris [38] . CF was diluted 1:10 in distilled water (CF 0.1x) previous to its use.…”

Section: Cf Extract Obtentionmentioning

confidence: 99%

“…In this study, we propose a novel concept of BCA that would induce the effect in plants that vaccines have in humans as if it was a "phytovaccine". This BCA consists of a heat-inactivated culture of a strawberry fungal pathogen, namely culture filtrate (CF) [38] . Thus, this study aimed to evaluate the capacity of CF to biocontrol grey mould rot in postharvest strawberries and plants while promoting plant growth.…”

Section: Introductionmentioning

confidence: 99%

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Biocontrol and Biostimulant Effect of a Fungal-Derived Extract in Commercial Varieties of Strawberry (Fragaria x ananassa Duch.)

Conrad,

Ricci,

Salazar

2022

J Mod Agric Biotechnol

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Objective: Strawberry postharvest fruits and plants are mainly affected by the fungus Botrytis cinerea (B. cinerea), which causes grey mould rot. Biological control agents and biostimulants can effectively control this fungus and promote plant growth. This study aimed to evaluate the capacity of a novel fungal-derived extract called culture filtrate (CF) to biocontrol grey mould rot in postharvest strawberries and plants while promoting plant growth. Methods:The CF extract was obtained from the liquid culture of a strawberry pathogenic fungus, gauzefiltered and inactivated by autoclave. The Fragaria x ananassa cultivars Camino Real, Benicia, and Merced were used. For the postharvest assay, fruit natural decay was evaluated by simulating real storage conditions. For the phytopathological assays, first-generation plants inoculated with B. cinerea were evaluated 26 days post infection. The effect of CF on plant growth and biomass promotion was evaluated 60 days post treatment (DPT). Results:The treatment with CF yielded healthier fruits of the three varieties, which presented minor severity of grey mould symptoms compared to the controls treated with water or potato dextrose broth (PDB) by 7 DPT. For instance, 40% of Camino Real, 80% of Benicia, and 20% of Merced fruits presented an incipient lesion (severity 1), whereas no fruits treated with water or PDB showed this minor level of infection. On the contrary, around 50% and 70% of the control fruits presented the highest level of severity. Moreover, CF pre-treatment of Benicia "mother plants" protected first-generation untreated plants. Likewise, the best biostimulant effect was observed for Benicia plants, which presented an increased number of leaves and runner production 60 DPT. Conclusion:The fungal-derived extract CF constitutes a promising alternative to be used as a biological Innovation Forever Publishing Group

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Fungal-derived extracts induce resistance against Botrytis cinerea in Arabidopsis thaliana

Cited by 5 publications

References 52 publications

Insight into the Microbiological Control Strategies against Botrytis cinerea Using Systemic Plant Resistance Activation

Insight into the Microbiological Control Strategies against Botrytis cinerea Using Systemic Plant Resistance Activation

Phytovaccines, a New Paradigm for Crop Protection: Towards a Sustainable Agriculture

Biocontrol and Biostimulant Effect of a Fungal-Derived Extract in Commercial Varieties of Strawberry (Fragaria x ananassa Duch.)

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