Functionalized silica shell magnetic nanoparticles for nanophase peptide synthesis applications

Microwave-Assisted Sol–Gel Preparation of the Nanostructured Magnetic System for Solid-Phase Synthesis

“…The peptide synthesis was performed according to a previously described protocol [9], by a nanophase step by step AA (amino acids) chain build-up, followed by the final TFA cleavage. The crude reaction product (Val-Ile-Lys) was analyzed using direct sample introduction (syringe infusion) by HR-MS (FT-ICR).…”

“…The efficiency of the newly synthesized nanostructured system MW-NPS-2-APS-HMBA was highlighted by using it as a solid support in nanophase peptide synthesis (nanostructured support synthesis-NSS, Table 3). The use of magnetically nanostructured solid support overcomes both the problem of solubility, giving the advantage of the access of the reactants to the reaction center and a rapid separation of intermediate The peptide sequence Val-Ile-Lys was synthesized according to a protocol described earlier [9] using as solid support the nanostructured system MW-NPS-2.…”

“…Several researchers have focused in recent years on nanophase peptide synthesis (NphPS) as a sustainable alternative to classic SPhS, mainly because of the significantly higher surface area and facilitated diffusional access of the used reagents at the reaction center and of significantly operational advantages [9][10][11].…”

“…The present study is based on our previous work [9], related to nanophase peptide synthesis, focusing on the nanophase support synthesis optimization by an MW-assisted process, on more accessible linker systems and on the advantage of a higher purity of the final product by the TFA cleavage of the HMBA linker-peptide system. Additionally, an improved characterization of the final peptide by FT-ICR HR-MS analysis was performed, to prove the VIK sequence.…”

Di‐functional magnetic nanoflowers: A highly efficient support for immobilizing penicillin G acylase

“…In recent years, magnetic supports for immobilizing PGA have attracted extensive attention due to its easy and rapid separation, which was separated from mixture reaction system by a magnetic force, avoiding the loss of biocatalysts during separation and washing mechanical operation. In general, Fe 3 O 4 nanoparticles are employed as magnetic sources to design and prepare magnetic supports 3–6 . Chen et al 7 synthesized paramagnetic polymer microspheres as support immobilizing PGA through polymerization of glycidyl methacrylate, ally glycidylether, and methacrylamide on the surface of silica‐coated Fe 3 O 4 nanoparticles.…”