Functionalized Selenium Nanoparticles Synergizes With Metformin to Treat Breast Cancer Cells Through Regulation of Selenoproteins

Yu, Yang; Zhang, Zehang; Chen, Qi; You, Yi; Li, Xiaoling; Chen, Tianfeng

doi:10.3389/fbioe.2021.758482

Cited by 9 publications

(6 citation statements)

References 56 publications

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“…Selenium nanoparticles mainly exert an antitumor effect by inducing apoptosis . The antioxidant activity of selenium nanoparticles could modulate the metabolism of tumor cells to inhibit cell proliferation. , Moreover, studies have proved that selenium can mobilize immunity to resist tumors. − Various mechanisms are involved in the antitumor activity of selenium nanoparticles. More complicated antitumor mechanisms of selenium nanoparticles are under study.…”

Section: Discussionmentioning

confidence: 99%

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Construction of Selenium Nanoparticle-Loaded Mesoporous Silica Nanoparticles with Potential Antioxidant and Antitumor Activities as a Selenium Supplement

et al. 2022

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Excessive reactive oxygen species (ROS) can damage cells and affect normal cell functions, which are related to various diseases. Selenium nanoparticles are a potential selenium supplement for their good biocompatibility and antioxidant activity. However, their poor stability has become an obstacle for further applications. In this study, mesoporous silica nanoparticles (MSNs) were prepared as a carrier of selenium nanoparticles. Pluronic F68 (PF68) was used for the surface modification of the compounds to prevent the leakage of the selenium nanoparticles. The prepared MSN@Se@PF68 nanoparticles were characterized by transmission electron microscopy, energy-dispersive X-ray spectroscopy, dynamic light scattering, X-ray photoelectron spectroscopy, confocal micro-Raman spectroscopy, and Fourier transform infrared spectroscopy. The MSN@Se@PF68 nanoparticles showed excellent antioxidant activity in HeLa tumor cells and zebrafish larvae. The cytotoxicity of MSN@Se@PF68 nanoparticles was concentration- and time-dependent in HeLa tumor cells. The MSN@Se@PF68 nanoparticles showed a negligible cytotoxicity of ≤2 μg/mL at 48 h. At a concentration of 50 μg/mL, the cell viability of the HeLa tumor cells decreased to about 50%. The results indicated that the MSN@Se@PF68 nanoparticles could be a potential antitumor agent. The embryonic development of zebrafish cocultured with the MSN@Se@PF68 nanoparticles showed that there was no lethal or obvious teratogenic toxicity. The results implied that the MSN@Se@PF68 nanoparticles could be a safe selenium supplement and have the potential for antioxidant and antitumor activity.

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Section: Discussionmentioning

confidence: 99%

“… 54 The antioxidant activity of selenium nanoparticles could modulate the metabolism of tumor cells to inhibit cell proliferation. 55 , 56 Moreover, studies have proved that selenium can mobilize immunity to resist tumors. 57 − 59 Various mechanisms are involved in the antitumor activity of selenium nanoparticles.…”

Section: Discussionmentioning

confidence: 99%

Construction of Selenium Nanoparticle-Loaded Mesoporous Silica Nanoparticles with Potential Antioxidant and Antitumor Activities as a Selenium Supplement

et al. 2022

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“…The effect of the LNT-SeNP treatment on CT26 cell-cycle distribution was analyzed by flow cytometry according to the published procedure [ 26 ]. After the incubation with LNT-SeNPs for 72 h, the cells were fixed with 75% ethanol overnight at −20 °C, and then stained with PI at 25 °C for 20–30 min in darkness.…”

Section: Methodsmentioning

confidence: 99%

“…CT26 cells were treated with LNT-SeNPs at the concentrations of 5 μM, 10 μM, or 20 μM, followed by incubation with a mitochondrial membrane potential probe (JC-1) at 37 °C for 30 min [ 26 ]. After incubation, the morphological changes were assessed using flow cytometry.…”

Section: Methodsmentioning

confidence: 99%

Theranostic Cancer Treatment Using Lentinan-Coated Selenium Nanoparticles and Label-Free CEST MRI

Liu

Ling

et al. 2022

Pharmaceutics

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Selenium nanoparticle (SeNP)-based nanotherapeutics have become an emerging cancer therapy, while effective drug delivery remains a technical hurdle. A theranostic approach, through which imaging companions are integrated with SeNPs, will allow image-guided drug delivery and, therefore, is highly desirable. Traditional methods require the chemical conjugation of imaging agents to the surface of nanoparticles, which may impede the later clinical translation. In this study, we developed a label-free strategy in which lentinan-functionalized SeNPs (LNT-SeNPs) are detected using MRI by the hydroxyl protons carried on LNT molecules. The in vitro phantom study showed that LNT and LNT-SeNPs have a strong CEST signal at 1.0 ppm apart from the water resonance, suggesting an in vivo detectability in the µM concentration range. Demonstrated on CT26 colon tumor cells, LNT-SeNPs exert a strong anticancer effect (IC50 = 4.8 μM), prominently attributed to the ability to generate intracellular reactive oxygen species. However, when testing in a mouse model of CT26 tumors, administration of LNT-SeNPs alone was found unable to deliver sufficient drugs to the tumor, leading to poor treatment responses. To improve the drug delivery, we co-injected LNT-SeNPs and TNF-α, a previously reported drug that could effectively damage the endothelial cells in the tumor vasculature, thereby increasing drug delivery to the tumor. Our results revealed a 75% increase in the intratumoral CEST MRI signal, indicating a markedly increased delivery efficiency of LNT-SeNPs when combined with TNF-α. The combination therapy also resulted in a significantly enhanced treatment outcome, as revealed by the tumor growth study. Taken together, our study demonstrates the first label-free, SeNP-based theranostic system, in which LNT was used for both functional surface coating and CEST MRI signal generating. Such a theranostic LNT-SeNP system is advantageous because it requires chemical labeling and, therefore, has high biocompatibility and low translatable barriers.

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“…Modifications to AMPK expression influenced mitochondrial membrane potential for a synergistic impact. 110…”

Section: Introductionmentioning

confidence: 99%

Plant-derived selenium nanoparticles: investigating unique morphologies, enhancing therapeutic uses, and leading the way in tailored medical treatments

Puri,

Mohite,

Ansari

et al. 2024

Mater. Adv.

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Functionalized Selenium Nanoparticles Synergizes With Metformin to Treat Breast Cancer Cells Through Regulation of Selenoproteins

Cited by 9 publications

References 56 publications

Construction of Selenium Nanoparticle-Loaded Mesoporous Silica Nanoparticles with Potential Antioxidant and Antitumor Activities as a Selenium Supplement

Construction of Selenium Nanoparticle-Loaded Mesoporous Silica Nanoparticles with Potential Antioxidant and Antitumor Activities as a Selenium Supplement

Theranostic Cancer Treatment Using Lentinan-Coated Selenium Nanoparticles and Label-Free CEST MRI

Plant-derived selenium nanoparticles: investigating unique morphologies, enhancing therapeutic uses, and leading the way in tailored medical treatments

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