Functional study of Villin 2 protein expressed in longissimus dorsi muscle of Korean native cattle in different growth stages

Abstract

The aim of this study was to investigate protein profiles related to the induction of adipogenesis within the bovine longissimus dorsi muscle (BLDM) by proteomic analysis. We analyzed BLDM proteins at different growth stages to clarify the physiological mechanisms of marbled muscle development in 20 head of Korean native cattle (11 month: 10 head, 17 month: 10 head). BLDM proteins were analyzed by two-dimensional electrophoresis and image analysis. Villin 2 was specifically identified by mass spectrometry and a protein search engine. Villin 2 protein expression in BLDM decreased during the fat development stage in test steers. In a Western blot cell culture study of spontaneously immortal bovine muscle fibroblasts, the abundance of Villin 2 was shown to be down-regulated during differentiation into muscle. In 3T3-L1 mouse embryonic fibroblasts, Villin 2 was decreased during differentiation into adipocytes. The results suggest that Villin 2 may be related to the induction of transdifferentiation and adipogenesis in bovine longissimus dorsi muscle. [BMB reports 2012; 45(2): 102-107]

Lithium chloride’s inhibition of 3T3-L1 cell differentiation by regulating the Wnt/β-catenin pathway and enhancing villin 2 expression

“…The results reveal that the cell structure may not be affected; thus, villin 2 is related with adipocyte differentiation. The result also confirmed that villin 2 is the downregulated during adipocyte differentiation (1).…”

“…2F). Moreover, in Jin's study, villin 2 protein is sharply down-regulated in 3T3-L1 mouse embryonic fibroblasts after 2 days of differentiation induction (1). Therefore, 20 mM LiCl may open Wnt/β-catenin signaling after 2 days.…”

“…Previous proteomics studies indicate that villin 2 protein is down-regulated in adipogenesis of the muscle tissue of Korean native steers (1). Moreover, villin 2 protein is sharply down-regulated in 3T3-L1 mouse embryonic fibroblasts after 2 days of differentiation induction (1). Therefore, we surmise that villin 2 is related to adipogenesis by regulating cytoskeleton associated proteins (2)(3)(4).…”

“…A wide range of research topics on lipid synthesis and metabolism are available to study. Previous proteomics studies indicate that villin 2 protein is down-regulated in adipogenesis of the muscle tissue of Korean native steers (1). Moreover, villin 2 protein is sharply down-regulated in 3T3-L1 mouse embryonic fibroblasts after 2 days of differentiation induction (1).…”