Functional role of miR-27b in the development of gastric cancer

Abstract

Previous studies have demonstrated that microRNAs (miRNAs/miRs) act as tumor suppressors or oncogenes during multiple processes in cancer. It has been observed that miR-27b may act as a tumor-suppressor and was significantly downregulated in a number of types of cancer. However, the functions of miR-27b in gastric cancer (GC) remain unclear. The present study aimed to investigate the functional role of miR-27b in the progression of GC. The downregulation of miR-27b in human GC plasma was confirmed using miRNA microarray and reverse transcription-quantitative polymerase chain reaction analyses. The association between circulating miR-27b expression and clinicopathological features of GC was analyzed and the results demonstrated that the level of circulating miR-27b was significantly correlated with GC differentiation. Receiver operating characteristic curve analysis identified that the plasma level of miR-27b may be a potential biomarker for differentiating patients with GC from healthy controls. In order to investigate the effect of miR-27b on GC cell behavior, miR-27b was overexpressed using miR-27b mimics, and it was observed that miR-27b was able to inhibit cell proliferation and induce apoptosis in SGC7901 cells. Previous studies have demonstrated that vascular endothelial growth factor C (VEGFC) is a target of miR-27b, and the results of the present study were consistent with these reports. Taken together, the results of the present study indicated that miR-27b may act as a potential biomarker for differentiating patients with GC from healthy controls, and serve as a tumor suppressor in GC by targeting VEGFC.

Literature-Based Enrichment Insights into Redox Control of Vascular Biology

“…Current research to a certain extent supports this hypothesis, as Pan et al reported overexpression of ZFAS1 in gastric cancer (GC) serum and tissue samples and demonstrated that ZFAS1 knockdown inhibits the proliferation and migration of GC cells by suppressing cell cycle progression and apoptosis [150], while Chen et al demonstrated that miR-27b is downregulated in GC and show miR-27b to be a potential GC biomarker. Moreover, they show that miR-27b functions as a tumor suppressor in GC by targeting VEGFC [151]. This shows a possible inverse relationship between ZFAS1 and miR-27b.…”

Effect of miR-27b-5p on apoptosis of human vascular endothelial cells induced by simulated microgravity

“…Numerous studies have revealed the relationship between miR-27b-5p and gene expression. Chen et al observed that miR-27b inhibited cell proliferation and induce apoptosis in gastric cancer cells [33]. Wu et al demonstrated that lncRNA ZEB2-AS1 promoted tumorigenesis and development of bladder cancer through down-regulating tumor-suppressive miR-27b [34].…”

Up-Regulated MicroRNA-27b Promotes Adipocyte Differentiation via Induction of Acyl-CoA Thioesterase 2 Expression

“…Among altered miRNAs in patients, we found that miR-27b is remarkedly increased in liver tissue and serum from the early phase of NAFLD to severe NASH [12,13]. It has been reported that miR-27b is involved in cell proliferation, differentiation, migration, invasion, metastasis, multi-drug resistance in various tumor cells [14][15][16][17][18]. However, the exact role of miR-27b in NAFLD pathogenesis, and in particular its relationship to adipocyte differentiation, remains unclear.…”