2015
DOI: 10.18632/aging.100716
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Abstract: Background: Bone marrow multipotent mesenchymal stromal cells (BM-MMSC) considered as a prospective substrate for cell therapy applications, however adult stem cells could be affected by donor-specific factors: age, gender, medical history. Our aim was to investigate how HF affects the functional properties of BM-MMSC. Materials and methods: BM-MMSC from 10 healthy donors (HD), and 16 donors with chronic HF were evaluated for proliferative activity, ability to differentiate, replicative senescence, expression … Show more

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Cited by 15 publications
(16 citation statements)
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References 64 publications
(61 reference statements)
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“…Surprisingly, the osteogenic potential of adipose MSC did not correlate with the calcification of the aortic valve. It has been previously reported that the osteogenic potential of MSC drops significantly with age and with cardiovascular pathology as well (Dmitrieva et al, 2015). In this study, the group of patients with CAVD was indeed older then the control groups for both VICs and MSCs.…”
Section: Medicinesupporting
confidence: 54%
See 1 more Smart Citation
“…Surprisingly, the osteogenic potential of adipose MSC did not correlate with the calcification of the aortic valve. It has been previously reported that the osteogenic potential of MSC drops significantly with age and with cardiovascular pathology as well (Dmitrieva et al, 2015). In this study, the group of patients with CAVD was indeed older then the control groups for both VICs and MSCs.…”
Section: Medicinesupporting
confidence: 54%
“…As control we used adipose tissue-derived MSCs and VICs derived from healthy donors. We first immunophenotyped MSCs and VICs from the patients and healthy donors with conventional MSC markers (Zuk et al, 2001;Dmitrieva et al, 2015). We did not observe significant differences either between the two types of cells or between the patients and healthy donors (Fig.…”
Section: Resultsmentioning
confidence: 91%
“…After expansion the cells were maintained in Petri dishes (Corning, USA) in growth medium, at 37°C and 5% CO 2 . cMSC immunophenotype was verified with flow cytometer GuavaEasyCyte6 (Millipore, USA) using CD33, CD45, CD117, CD90, CD105, CD73, CD146, CD56 monoclonal antibodies (BD, USA) as previously described (Dmitrieva et al 2015).…”
Section: Cell Culturementioning
confidence: 99%
“…The PDTs of MSCs from donors with CHF and cultured in autologous serum‐containing medium were longer than those of MSCs from donors without CHF and cultured in FBS‐containing medium. Previous studies have also reported (Dmitrieva et al ., ) that MSCs from donors with CHF exhibited significantly longer PDTs than MSCs from donors without CHF. In contrast, MSCs from donors without CHF ( n = 27) and with CHF ( n = 3) showed similar PDTs when FBS was used in the culture medium (Figure ).…”
Section: Surface Antigens Present On Cultured Adherent Cells From Bonmentioning
confidence: 99%
“…Therefore, we evaluated whether the donor origin of cells and/or culture conditions contributed to differences in growth rates of MSCs, which may be critical to know for future MSCbased regenerative medicine. Several previous studies have evaluated the potential relationships between donor age and the growth of MSCs (Fickert et al, 2011;Li et al, 2014;Phinney et al, 1999;Siegel et al, 2013), the growth of MSCs from donors with and without CHF (Dmitrieva et al, 2015), as well as the effect of different types of serum in the culture medium on the growth of MSCs (Kuznetsov et al, 2000;Shahdadfar et al, 2005;Stute et al, 2004). Whereas some studies have reported a positive correlation with age, others have shown a lack of correlation.…”
mentioning
confidence: 99%