Functional Mitochondria Are Important for the Effect of Resveratrol

Widlund, Anne L.; Baral, Kaushal; Dalgaard, Louise Torp; Vang, Ole

doi:10.3390/molecules22050847

Cited by 9 publications

(9 citation statements)

References 30 publications

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“…Resveratrol is believed to mimic the physiological effects of CR in a mainly SIRT1-or SIRT3-dependent manner [22,23]. Functional mitochondria have been reported to be important for the effects of RSV [24]. Thus, in the present study, this potential effect of RSV was evaluated in oxidative phosphorylation capacities and transcription factors involved in mitochondrial biogenesis in myoblasts of five patients harboring the m.3243A>G point mutation and five controls.…”

Section: Discussionmentioning

confidence: 97%

The Effect of Resveratrol on Mitochondrial Function in Myoblasts of Patients with the Common m.3243A>G Mutation

et al. 2020

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Mitochondrial function is essential for ATP-supply, especially in response to different cellular stressors. Increased mitochondrial biogenesis resulting from caloric restriction (CR) has been reported. Resveratrol (RSV) is believed to mimic the physiological effects of CR mainly via a sirtuin (SIRT) 1-dependent pathway. The effect of RSV on the physiological function of mitochondrial respiratory complexes was evaluated using a Seahorse XF96. Myoblasts of five patients harboring the m.3243A>G mutation and five controls were analyzed. The relative expression of several genes involved in mitochondrial biogenesis was evaluated for a better understanding of the coherent mechanisms. Additionally, media-dependent effects of nutritional compounds and hormonal restrictions (R) on myoblasts from patients and controls in the presence or absence of RSV were investigated. Culturing of myoblasts under these conditions led to an upregulation of almost all the investigated genes compared to normal nutrition. Under normal conditions, there was no positive effect of RSV on mitochondrial respiration in patients and controls. However, under restricted conditions, the respiratory factors measured by Seahorse were improved in the presence of RSV. Further studies are necessary to clarify the involved mechanisms and elucidate the controversial effects of resveratrol on SIRT1 and SIRT3 expression.

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Section: Discussionmentioning

confidence: 97%

The Effect of Resveratrol on Mitochondrial Function in Myoblasts of Patients with the Common m.3243A>G Mutation

et al. 2020

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“…In non-alcoholic fatty liver disease (NAFLD), an upregulation of a fibrinogen is an independent risk factor of CVD. Studies with trans-resveratrol have shown that this polyphenol reduces the liver fat and hepatic enzymes such as glutamate pyruvic transaminase (SGPT) and gamma-glutamyl transpeptidase (g-GT), and it also ameliorates insulin resistance, thus preventing and reducing liver damage in NAFLD [34][35][36][37].…”

Section: Anti-oxidative Effects Of Fibrinogen/resveratrol Complexmentioning

confidence: 99%

Fibrinogen Increases Resveratrol Solubility and Prevents it from Oxidation

Gligorijević

Radomirović

Rajković

et al. 2020

Foods

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The French paradox describes a lower incidence of cardiovascular problems despite a high intake of saturated fats. This phenomenon was associated with higher consumption of red wine, as it was later discovered that the presence of antioxidants, including resveratrol, have beneficial effects. We hypothesized that resveratrol may have a more direct role in protection from harmful oxidation, presumably through binding to important proteins of the blood coagulation process. Spectrofluorimetry demonstrated that resveratrol is capable of binding to fibrinogen, the main protein in the coagulation process, which is also important as a food additive. Various spectroscopic methods determined that binding does not cause fibrinogen unfolding or destabilization since protein melting temperature remains unchanged. A mutually protective effect against the free radical-induced oxidation of polyphenol and fibrinogen was found. The presence of fibrinogen caused only a negligible masking effect of the antioxidative abilities of resveratrol, measured by a reduction of hexacyanoferrate (III), while greatly increasing its solubility in an aqueous environment, thus increasing its potential bioavailability. Due to its interaction with fibrinogen, resveratrol may serve as an antioxidant at the site of injury. The antioxidative effect of resveratrol may also protect and thus keep the desired characteristics of fibrinogen during the application of this protein as a food additive.

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“…This huge, diverse group of plant metabolites includes well-known compounds, such as the phytoalexin, resveratrol (3,4′,5-trihydroxy- trans -stilbene; RSV) and the flavonoid, quercetin [ 2 ]. The actions of RSV have been studied in several in vivo and in vitro approaches, demonstrating antioxidant, anti-inflammatory and anti-aging effects [ 3 ], but also pro-apoptotic anti-cancer properties [ 4 , 5 ] as well as antimicrobial activity [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…By hosting the complexes of the respiration chain in their inner membranes (IMM), mitochondria are the predominant source for the cell’s most important energy carrier, adenosine triphosphate (ATP). Consequently, the control of mitochondrial activity is of utmost importance for cellular wellbeing [ 4 ] and, obviously also for the therapeutic action of RSV, as experiments in mitochondrial DNA deficient Rho 0 cells have revealed functional mitochondria to be crucial for the effects of RSV to occur [ 5 ]. Interestingly, experiments revealed a strong concentration-dependent impact of RSV on mitochondrial function, which is of major importance, due to the therapeutic potential of this polyphenol [ 6 , 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Dosis Facit Sanitatem—Concentration-Dependent Effects of Resveratrol on Mitochondria

2017

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The naturally occurring polyphenol, resveratrol (RSV), is known for a broad range of actions. These include a positive impact on lifespan and health, but also pro-apoptotic anti-cancer properties. Interestingly, cell culture experiments have revealed a strong impact of RSV on mitochondrial function. The compound was demonstrated to affect mitochondrial respiration, structure and mass of mitochondria as well as mitochondrial membrane potential and, ultimately, mitochondria-associated cell death pathways. Notably, the mitochondrial effects of RSV show a very strict and remarkable concentration dependency: At low concentrations, RSV (<50 μM) fosters cellular antioxidant defense mechanisms, activates AMP-activated protein kinase (AMPK)- and sirtuin 1 (SIRT1)-linked pathways and enhances mitochondrial network formation. These mechanisms crucially contribute to the cytoprotective effects of RSV against toxins and disease-related damage, in vitro and in vivo. However, at higher concentrations, RSV (>50 μM) triggers changes in (sub-)cellular Ca2+ homeostasis, disruption of mitochondrial membrane potential and activation of caspases selectively yielding apoptotic cancer cell death, in vitro and in vivo. In this review, we discuss the promising therapeutic potential of RSV, which is most probably related to the compound’s concentration-dependent manipulation of mitochondrial function and structure.

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Functional Mitochondria Are Important for the Effect of Resveratrol

Cited by 9 publications

References 30 publications

The Effect of Resveratrol on Mitochondrial Function in Myoblasts of Patients with the Common m.3243A>G Mutation

The Effect of Resveratrol on Mitochondrial Function in Myoblasts of Patients with the Common m.3243A>G Mutation

Fibrinogen Increases Resveratrol Solubility and Prevents it from Oxidation

Dosis Facit Sanitatem—Concentration-Dependent Effects of Resveratrol on Mitochondria

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