Functional Interactions of the RNA Polymerase II-interacting Proteins Gdown1 and TFIIF

Davis, Melissa A. Mullen; Guo, Jiannan; Price, David H.; Luse, Donal S.

doi:10.1074/jbc.m113.544395

Cited by 15 publications

(17 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…39); or the combined action of XRN2 and the Microprocessor complex 40 . Moreover, GDOWN1 (also known as GRINL1A) is thought to increase pausing by blocking the interaction of TFIIF with Pol II, but also by inhibiting termination of Pol II by TTF2 (REFS 41–43). However, genome-wide measurements of the stability of paused Pol II in D. melanogaster cells 30 and mouse embryonic stem cells 9 indicate that Pol II has a stable average half-life of ~7 minutes, or almost 1 hour in a small subset of genes 44 .…”

Section: Mechanisms That Contribute To Pausingmentioning

confidence: 99%

“…GDOWN1, which tightly interacts with Pol II, may increase pausing by interfering with the binding of the positive elongation factor TFIIF 41–43 . Inhibiting P-TEFb activity can also increase pausing by decreasing P-TEFb-mediated Pol II release.…”

Section: Factors Involved In Setting Up Pausingmentioning

confidence: 99%

“…For instance, phosphorylation of Ser2 of the Pol II CTD does not reach a maximum until a few kilobases into the gene body 104 . Furthermore, pausing factors, such as NELF, DSIF and GDOWN1 (REFS 1,41–43), may be gradually transformed or removed from the transcriptional machinery. This gradual ‘maturation’ of the transcription machinery could facilitate the recruitment of factors that are important for co-transcriptional events such as splicing.…”

Section: Moving On To Productive Elongationmentioning

confidence: 99%

See 2 more Smart Citations

Getting up to speed with transcription elongation by RNA polymerase II

Jonkers

Lis

2015

Nat Rev Mol Cell Biol

703

768

View full text Add to dashboard Cite

Recent advances in sequencing techniques that measure nascent transcripts and that reveal the positioning of RNA polymerase II (Pol II) have shown that the pausing of Pol II in promoter-proximal regions and its release to initiate a phase of productive elongation are key steps in transcription regulation. Moreover, after the release of Pol II from the promoter-proximal region, elongation rates are highly dynamic throughout the transcription of a gene, and vary on a gene-by-gene basis. Interestingly, Pol II elongation rates affect co-transcriptional processes such as splicing, termination and genome stability. Increasing numbers of factors and regulatory mechanisms have been associated with the steps of transcription elongation by Pol II, revealing that elongation is a highly complex process. Elongation is thus now recognized as a key phase in the regulation of transcription by Pol II.

show abstract

Section: Mechanisms That Contribute To Pausingmentioning

confidence: 99%

Section: Factors Involved In Setting Up Pausingmentioning

confidence: 99%

Section: Moving On To Productive Elongationmentioning

confidence: 99%

See 1 more Smart Citation

Getting up to speed with transcription elongation by RNA polymerase II

Jonkers

Lis

2015

Nat Rev Mol Cell Biol

703

768

View full text Add to dashboard Cite

show abstract

“…Current pausing models suggest that in order for Pol II to stably pause, another factor called Gdown1 associates with Pol II immediately after initiation and displaces TFIIF from Pol II. [33][34][35][36] A recent Cryo-EM study showed that TFIIF and Gdown1 interact with Pol II in a mutually exclusive manner as they bind to overlapping surfaces and likely sterically compete with each other. 37 Although speculative, the steric competition model may extend to SEC where it competes with Gdown1 using its TFIIF-like domains to alleviate pausing and reactivate elongation (Fig.…”

Section: Resultsmentioning

confidence: 99%

Super elongation complex contains a TFIIF-related subcomplex

et al. 2016

View full text Add to dashboard Cite

Super elongation complex (SEC) belongs to a family of RNA polymerase II (Pol II) elongation factors that has similar properties as TFIIF, a general transcription factor that increases the transcription elongation rate by reducing pausing. Although SEC has TFIIF-like functional properties, it apparently lacks sequence and structural homology. Using HHpred, we find that SEC contains an evolutionarily related TFIIF-like subcomplex. We show that the SEC subunit ELL interacts with the Pol II Rbp2 subunit, as expected for a TFIIF-like factor. These findings suggest a new model for how SEC functions as a Pol II elongation factor and how it suppresses Pol II pausing.

show abstract

“…Promoter-proximal pausing was later shown to require DSIF (Wada et al, 1998) and NELF (Yamaguchi et al, 1999), which cooperatively reduce the Pol II elongation rate (Renner et al, 2001). Paused polymerases which contain the substoichiometric Pol II subunit Gdown1 are also resistant to both elongation stimulation by TFIIF and termination by TTF2 (Cheng et al, 2012;Guo et al, 2014b;Mullen Davis et al, 2014). In a factor-dependent manner, these abortive elongation complexes could transition into a mode of "productive elongation" and generate full-length transcripts (Marshall and Price, 1992); this factor was later purified and named positive transcription elongation factor b (P-TEFb) (Marshall and Price, 1995).…”

Section: Transcription Elongation Controlmentioning

confidence: 99%

Regulation of Pol II transcription and mRNA capping

Nilson¹

View full text Add to dashboard Cite

I would also like to thank past and current members of the Price Lab for creating and maintaining a unique research environment. Dr. Jiannan Guo properly introduced me to biochemistry and taught me how to perform in vitro transcriptions. John Brogie regularly challenged my sanity and provided hours of discussion. I would like to thank my neighbor and friend Dr. Ruth Spinks for her encouragement through difficult and challenging times. Without her support, this thesis would not exist. I would like to thank the University of Iowa Graduate College for awarding me the Presidential Graduate Research Fellowship, which gave me the freedom to pursue ambitious and expensive experiments even when funding was questionable. Finally, I would like to thank my parents Donald and Kathleen Nilson.

show abstract

Functional Interactions of the RNA Polymerase II-interacting Proteins Gdown1 and TFIIF

Cited by 15 publications

References 39 publications

Getting up to speed with transcription elongation by RNA polymerase II

Getting up to speed with transcription elongation by RNA polymerase II

Super elongation complex contains a TFIIF-related subcomplex

Regulation of Pol II transcription and mRNA capping

Contact Info

Product

Resources

About