Functional insights into voltage gated proton channel (Hv1) in bull spermatozoa

Mishra, Abhishek Kumar; Kumar, Akshay; Yadav, Sarita; Anand, Mukul; Yadav, Brijesh; Nigam, Rajesh; Garg, Satish K.; Swain, Dilip Kumar

doi:10.1016/j.theriogenology.2019.06.015

Cited by 19 publications

(40 citation statements)

References 23 publications

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“…HVCN1 channels have an essential regulating role in several cell types (reviewed in [50]) by driving protons unidirectionally to the extracellular medium [30], despite their functional significance in mammalian sperm being poorly understood. While HVCN1 channels are present in the plasma membrane of human, macaque, cattle and pig sperm, they are absent from mouse sperm [1,23,24,46,51]. Remarkably, in macaques, sperm cryopreservation results in a decreased content of HVCN1 channels in the plasma membrane [48].…”

Section: Discussionmentioning

confidence: 99%

“…Blockade of HVCN1 channels during cryopreservation led to reduced sperm viability, expressed as spermatozoa exhibiting an intact plasma membrane integrity, and reduced sperm motility and kinematics after thawing. In previous studies, inactivation of HVCN1 channels has been reported to result in decreased sperm viability in ejaculated human [52] and bovine sperm [1], since this makes these cells unable to regulate their inner pH [23]. Nevertheless, little data exist about the changes in the intracellular pH throughout the cryopreservation procedure.…”

Section: Discussionmentioning

confidence: 99%

“…Blocking HVCN1 channels also leads to a decrease in the motility and velocity of in vitro capacitated sperm from pigs [26] and humans [22]. In bovine [1] and human sperm [23], these channels are essential for activation of progressive motility upon ejaculation and for hyperactivation during capacitation. Moreover, in humans, HVCN1 activity is higher in capacitated than in non-capacitated sperm due to the phosphorylation of this channel during capacitation [22,23].…”

Section: Discussionmentioning

confidence: 99%

“…Ion channels are essential for sperm physiology during spermatogenesis, epididymal maturation, and storage, transit throughout the female reproductive tract and interaction with the oocyte [1]. Since the first identification of cation sperm channels (CatSper) in the plasma membrane of mouse spermatozoa in 2001 [2], several studies have investigated different ion channels and their relevance for sperm function (reviewed in [3]).…”

Section: Introductionmentioning

confidence: 99%

“…While previous studies have immunolocalized both SLO1 potassium channels and voltage-gated proton channels (HVCN1) in the plasma membrane of mammalian spermatozoa (human [22][23][24], cattle [1], and pig [25,26]) and support a role for them during sperm capacitation, none has investigated whether they are involved in sperm cryotolerance. Therefore, the main objective of the present research is to elucidate the physiological role of SLO1 and HVCN1 channels during mammalian sperm cryopreservation.…”

Section: Introductionmentioning

confidence: 99%

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HVCN1 but Not Potassium Channels Are Related to Mammalian Sperm Cryotolerance

Delgado-Bermúdez

Mateo‐Otero

Llavanera

et al. 2021

IJMS

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Little data exist about the physiological role of ion channels during the freeze–thaw process in mammalian sperm. Herein, we determined the relevance of potassium channels, including SLO1, and of voltage-gated proton channels (HVCN1) during mammalian sperm cryopreservation, using the pig as a model and through the addition of specific blockers (TEA: tetraethyl ammonium chloride, PAX: paxilline or 2-GBI: 2-guanidino benzimidazole) to the cryoprotective media at either 15 °C or 5 °C. Sperm quality of the control and blocked samples was performed at 30- and 240-min post-thaw, by assessing sperm motility and kinematics, plasma and acrosome membrane integrity, membrane lipid disorder, intracellular calcium levels, mitochondrial membrane potential, and intracellular O2−⁻ and H2O2 levels. General blockade of K+ channels by TEA and specific blockade of SLO1 channels by PAX did not result in alterations in sperm quality after thawing as compared to control samples. In contrast, HVCN1-blocking with 2-GBI led to a significant decrease in post-thaw sperm quality as compared to the control, despite intracellular O2−⁻ and H2O2 levels in 2-GBI blocked samples being lower than in the control and in TEA- and PAX-blocked samples. We can thus conclude that HVCN1 channels are related to mammalian sperm cryotolerance and have an essential role during cryopreservation. In contrast, potassium channels do not seem to play such an instrumental role.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

HVCN1 but Not Potassium Channels Are Related to Mammalian Sperm Cryotolerance

Delgado-Bermúdez

Mateo‐Otero

Llavanera

et al. 2021

IJMS

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Interplay mechanisms between progesterone and endocannabinoid receptors in regulating bull sperm capacitation and acrosome reaction

Mahajan

Sharma

Mishra

et al. 2022

Journal Cellular Physiology

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After ejaculation, sperm show a limited capacity for transcription and translation. In the oviduct, most of the signalling in sperm is nongenomic and is mediated through membrane receptors. Studies have shown that the cation channel of sperm (CatSper), cAMP, cGMP, protein kinases, and tyrosine phosphorylation are involved in the nongenomic signalling of progesterone (P4) in sperm. However, it is not known whether there is an interplay between P4 and cannabinoid receptors 1 and 2 (CB1 and CB2), transient receptor potential vanilloid 1 (TRPV1), CatSper channels, cAMP, inositol trisphosphate receptor (IP3R), and mitogen-activated protein kinase (MAPK); these potential regulators are involved in the regulation of capacitation and the acrosome reaction. In the present study, selective blockers of CB1, CB2, TRPV1, CatSper channels, cAMP, protein kinase A (PKA), IP3R, and MAPK were used to identify their involvement in P4-mediated bull sperm capacitation and the acrosome reaction. Selective blocking of any one of the molecules caused a significant reduction in P4 signalling (p < 0.05). Interestingly, blocking these molecules in combination followed by treatment with P4 resulted in the complete absence of capacitation and the acrosome reaction. Blocking a single receptor was not able to eliminate the P4-induced capacitation and the acrosome reaction. In addition to the CB1 and CB2 receptors, there may be other signalling pathways that mediate P4 signalling. In conclusion, P4 signalling exhibited interplay with the cannabinoid receptors. The regulation of sperm capacitation and the acrosome reaction also involved cAMP, PKA, L-type and T-type calcium channels, TRPV1, inositol trisphosphate, and MAPK.

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Viscous Loading Regulates the Flagellar Energetics of Human and Bull Sperm

Yazdan Parast,

Veeraragavan,

Gaikwad

et al. 2023

Small Methods

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The viscoelastic properties of the female reproductive tract influence sperm swimming behavior, but the exact role of these rheological changes in regulating sperm energetics remains unknown. Using high‐speed dark‐field microscopy, the flagellar dynamics of free‐swimming sperm across a physiologically relevant range of viscosities is resolved. A transition from 3D to 2D slither swimming under an increased viscous loading is revealed, in the absence of any geometrical or chemical stimuli. This transition is species‐specific, aligning with viscosity variations within each species’ reproductive tract. Despite substantial drag increase, 2D slithering sperm maintain a steady swimming speed across a wide viscosity range (20–250 and 75–1000 mPa s for bull and human sperm) by dissipating over sixfold more energy into the fluid without elevating metabolic activity, potentially by altering the mechanisms of dynein motor activity. This energy‐efficient motility mode is ideally suited for the viscous environment of the female reproductive tract.

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Functional insights into voltage gated proton channel (Hv1) in bull spermatozoa

Cited by 19 publications

References 23 publications

HVCN1 but Not Potassium Channels Are Related to Mammalian Sperm Cryotolerance

HVCN1 but Not Potassium Channels Are Related to Mammalian Sperm Cryotolerance

Interplay mechanisms between progesterone and endocannabinoid receptors in regulating bull sperm capacitation and acrosome reaction

Viscous Loading Regulates the Flagellar Energetics of Human and Bull Sperm

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