Functional imaging of glucose-evoked rat islet activities using transient intrinsic optical signals

Yao, Xin Cheng; Cui, Wan Xing; Li, Yi Chao; Zhang, Wei; Lü, Rong; Thompson, Anthony; Amthor, Franklin R.; Wang, Xu Jing

doi:10.1080/09500340.2012.674564

Cited by 3 publications

(2 citation statements)

References 18 publications

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“…10 Stimulus-evoked IOSs correlated with action potentials and postsynaptic potentials have also been detected in excitable neural tissues 56,57 and endocrine cells. 58,59 Biophysical sources and physiological mechanisms of the IOSs in neural tissues have been explored by several research groups. 56,57,[60][61][62][63] Multiple physiological processes, such as neurotransmitter secretion, 64 reorientation of membrane proteins and phospholipids, 56,57,65 and refractive index change of neural tissues 66 during neural activation, might contribute to the observed IOSs observed in neural tissues.…”

Section: Discussionmentioning

confidence: 99%

Stimulus-evoked outer segment changes in rod photoreceptors

et al. 2016

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Abstract. Rod-dominated transient retinal phototropism (TRP) has been recently observed in freshly isolated mouse and frog retinas. Comparative confocal microscopy and optical coherence tomography revealed that the TRP was predominantly elicited from the rod outer segment (OS). However, the biophysical mechanism of rod OS dynamics is still unknown. Mouse and frog retinal slices, which displayed a cross-section of retinal photoreceptors and other functional layers, were used to test the effect of light stimulation on rod OSs. Time-lapse microscopy revealed stimulus-evoked conformational changes of rod OSs. In the center of the stimulated region, the length of the rod OS shrunk, while in the peripheral region, the rod OS swung toward the center region. Our experimental observation and theoretical analysis suggest that the TRP may reflect unbalanced rod disc-shape changes due to localized visible light stimulation.

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Section: Discussionmentioning

confidence: 99%

Stimulus-evoked outer segment changes in rod photoreceptors

et al. 2016

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“…Stimulus-evoked IOSs have been detected in the retina, neural tissues [10–13] and other excitable cells [14, 15]. Several imaging techniques, including fundus cameras [16, 17], AO ophthalmoscopes [18–20], OCT imagers [21–23] have been explored for functional IOS imaging of the retina.…”

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confidence: 99%

En face optical coherence tomography of transient light response at photoreceptor outer segments in living frog eyecup

Wang

Zhang

et al. 2013

Opt. Lett.

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This study was designed to test the feasibility of en face mapping of the transient intrinsic optical signal (IOS) response at photoreceptor outer segments and to assess the effect of spatial resolution on functional IOS imaging of retinal photoreceptors. A line-scan optical coherence tomography (LS-OCT) was constructed to achieve depth-resolved functional IOS imaging of living frog eyecups. Rapid en face OCT revealed transient IOS almost immediately (<3 ms) after the onset of visible light flashes at photoreceptor outer segments. Quantitative analysis indicated that the IOS kinetics may reflect dynamics of G-protein binding and releasing in early phases of visual transduction, and high resolution is essential to differentiate positive and negative IOS changes in adjacent locations.

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Intrinsic optical signal imaging of retinal physiology: a review

Yao

Wang

2015

J. Biomed. Opt

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Intrinsic optical signal (IOS) imaging promises to be a noninvasive method for high-resolution examination of retinal physiology, which can advance the study and diagnosis of eye diseases. While specialized optical instruments are desirable for functional IOS imaging of retinal physiology, in depth understanding of multiple IOS sources in the complex retinal neural network is essential for optimizing instrument designs. We provide a brief overview of IOS studies and relationships in rod outer segment suspensions, isolated retinas, and intact eyes. Recent developments of line-scan confocal and functional optical coherence tomography (OCT) instruments have allowed in vivo IOS mapping of photoreceptor physiology. Further improvements of the line-scan confocal and functional OCT systems may provide a feasible solution to pursue functional IOS mapping of human photoreceptors. Some interesting IOSs have already been detected in inner retinal layers, but better development of the IOS instruments and software algorithms is required to achieve optimal physiological assessment of inner retinal neurons.

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Functional imaging of glucose-evoked rat islet activities using transient intrinsic optical signals

Cited by 3 publications

References 18 publications

Stimulus-evoked outer segment changes in rod photoreceptors

Stimulus-evoked outer segment changes in rod photoreceptors

En face optical coherence tomography of transient light response at photoreceptor outer segments in living frog eyecup

Intrinsic optical signal imaging of retinal physiology: a review

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