Abstract:The gene products for the mitotic regulator genes RCC1 and Ran, p45rcc1 and p24ran, were expressed in Escherichia coli, purified in large amounts, and characterized for their biochemical properties. p24ran binds guanine nucleotide as a 1:1 complex, which is only slowly released from the protein. p45rcc1 catalyzes the exchange of nucleotide bound to the guanine nucleotide binding protein p24ran in the same way as the protein purified from HeLa cells. Likewise, the nucleotide dissociation from HeLa cell-derived … Show more
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