Functional differences between antiviral activities of sulfonated and intact intravenous immunoglobulin preparations toward varicella-zoster virus and cytomegalovirus

Yajima, Misako; Shiraki, Atsuko; Daikoku, Tohru; Oyama, Yukari; Yoshida, Yasuhiko; Shiraki, Kimíyasu

doi:10.1016/j.jiac.2015.01.012

Cited by 3 publications

(9 citation statements)

References 31 publications

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“…CMV (Towne strain)-infected cells were similarly suspended in PRMI-1640 with 10% FBS at a concentration of 2 • 10 5 cells/mL. We examined the effector-to-target (E:T) cell ratio of 100:1 and 30:1 in the ADCC assays to VZV-and CMVinfected cells with guinea pig spleen cells, and the E:T ratio of 100:1 showed better activity to VZV-and CMV-infected cells as previously reported (49). Aliquots of 0.1 mL of target cells (2 • 10 4 cells) were dispensed into round-bottomed tubes.…”

Section: Antibody-dependent Cellular Cytotoxicitymentioning

confidence: 85%

“…Human embryonic lung (HEL) cells were propagated in Eagle's minimum essential medium (MEM) that was supplemented with 10% heat-inactivated fetal bovine serum (FBS) and maintained in the same medium with 2% FBS. Human lung cancer A549 cells were grown in MEM with 5% FBS (33,37,49).…”

Section: Cells Viruses and Antiserummentioning

confidence: 99%

“…ADCC was examined as previously reported (49). Briefly, male Hartley guinea pigs (3 weeks old) were purchased from Sankyo Labs Service Co. Ltd., Tokyo, Japan.…”

Section: Antibody-dependent Cellular Cytotoxicitymentioning

confidence: 99%

“…To characterize the role of anti-CMV antibody, we compared the neutralization (NT) of varicella-zoster virus (VZV) and CMV with and without complement and found that both NT activities were enhanced by the complement. The NT antibody titer of IVIG toward VZV and CMV was enhanced about three to six times by the complement (49). Antibody to VZV showed ADCC toward VZV-infected cells, but anti-CMV antibody failed to show significant ADCC toward CMV-infected cells.…”

Section: Introductionmentioning

confidence: 97%

“…Maternal antibody prevents measles and varicella infection and alleviates CMV infection in neonates. Immunoglobulin (IgG) neutralizes viral infectivity with and without complement and mediates antibody-dependent cellular cytotoxicity (ADCC) toward infected cells (49). Thus, intravenous IgG (IVIG) is used to treat severe viral infections, especially CMV infections in congenitally CMV-infected (26) and immunosuppressed patients, such as transplant recipients (38,39,52).…”

Section: Introductionmentioning

confidence: 99%

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Interaction of Immunoglobulin with Cytomegalovirus-Infected Cells

et al. 2017

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Intravenous immunoglobulin (IVIG) is used to treat or prevent severe viral infection, especially cytomegalovirus (CMV) infections. IVIG was characterized to understand its interaction with CMV-infected cells. IVIG retarded CMV spread and reduced virus yields depending on the neutralizing (NT) antibody titer. Immediate early protein synthesis was reduced by IVIG in 3 to 15 h, and IVIG specifically reduced the ratio of 66/68k protein synthesis among immediate early proteins in an NT antibody-dependent manner between 4 and 8 h after infection, indicating that antigenic modulation of CMV-infected cells by IVIG reduced viral protein synthesis and virus production. The half-life of antibody bound to CMV-infected cells was 3.8 h. NT antibody titers to varicella-zoster virus (VZV) and CMV in IVIG were dose dependently absorbed by cells infected with VZV and CMV, respectively, but the antibody titers to CMV and VZV, respectively, were not affected. NT antibody in 0.3 mL of IVIG (15 mg) was specifically absorbed by 10 CMV-infected cells and 10 VZV-infected cells, suggesting that the NT antibody in IVIG might be inactivated by one-tenth of a similar volume of CMV-infected or VZV-infected cells. Various antiviral activities of IVIG may contribute to control and alleviation of CMV infection.

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Section: Antibody-dependent Cellular Cytotoxicitymentioning

confidence: 85%

Section: Cells Viruses and Antiserummentioning

confidence: 99%

“…ADCC was examined as previously reported (49). Briefly, male Hartley guinea pigs (3 weeks old) were purchased from Sankyo Labs Service Co. Ltd., Tokyo, Japan.…”

Section: Antibody-dependent Cellular Cytotoxicitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Interaction of Immunoglobulin with Cytomegalovirus-Infected Cells

et al. 2017

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Everolimus delayed and suppressed cytomegalovirus DNA synthesis, spread of the infection, and alleviated cytomegalovirus infection

et al. 2019

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Polyvalent human immunoglobulin for infectious diseases: Potential to circumvent antimicrobial resistance

Pedraza-Sánchez

Cruz-González²,

Rojas³

et al. 2023

Front. Immunol.

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Antimicrobial resistance (AMR) is a global health problem that causes more than 1.27 million deaths annually; therefore, it is urgent to focus efforts on solving or reducing this problem. The major causes of AMR are the misuse of antibiotics and antimicrobials in agriculture, veterinary medicine, and human medicine, which favors the selection of drug-resistant microbes. One of the strategies proposed to overcome the problem of AMR is to use polyvalent human immunoglobulin or IVIG. The main advantage of this classic form of passive immunization is its capacity to enhance natural immunity mechanisms to eliminate bacteria, viruses, or fungi safely and physiologically. Experimental data suggest that, for some infections, local administration of IVIG may produce better results with a lower dose than intravenous application. This review presents evidence supporting the use of polyvalent human immunoglobulin in AMR, and the potential and challenges associated with its proposed usage.

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Functional differences between antiviral activities of sulfonated and intact intravenous immunoglobulin preparations toward varicella-zoster virus and cytomegalovirus

Cited by 3 publications

References 31 publications

Interaction of Immunoglobulin with Cytomegalovirus-Infected Cells

Interaction of Immunoglobulin with Cytomegalovirus-Infected Cells

Everolimus delayed and suppressed cytomegalovirus DNA synthesis, spread of the infection, and alleviated cytomegalovirus infection

Polyvalent human immunoglobulin for infectious diseases: Potential to circumvent antimicrobial resistance

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