J. Neurosci. Res. volume 86, issue 13, P2884-2894 2008 DOI: 10.1002/jnr.21749 View full text
Madeline Nieves-Cintrón, Daniel Caballero-Rivera, Walter I. Silva, Manuel F. Navedo, José A. Lasalde-Dominicci

Abstract: The role of position L8′, located in transmembrane domain 1 of the neuronal nicotinic α3 subunit, was characterized by using two-electrode voltage clamp in Xenopus oocytes. Four amino acids (Ala, Ser, Phe, and Tyr) were inserted at this conserved position, and the mutant subunit was coexpressed with either wild-type β2 or β4 subunits. These substitutions led to significant alterations in the pharmacodynamic parameters of cholinergic agents, resulting in loss of function. Ala and Ser substitutions resulted in …

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