Functional characterization of the SbNrat1 gene in sorghum

Lu, Muxue; Wang, Zhigang; Fu, Shan; Yang, Guangsheng; Shi, Mingxing; Lu, Youshe; Wang, Xiaohu; Xia, Jixing

doi:10.1016/j.plantsci.2017.05.010

Cited by 18 publications

(13 citation statements)

References 25 publications

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“…The resulting phylogenetic tree includes five main clades corresponding to five distinctive Nramp groups ( Figure 1 ). Notably, two known transporters for trivalent Al ion, OsNrat1 ( Xia et al, 2010 , 2014 ; Li et al, 2014 ) and SbNrat1 ( Lu et al, 2017 ), an Nrat1-like transporter of Zea mays (ZmNrat1) as well, are located in the same clade (group III) but separated from other divalent ion transporters on the evolutionary tree.…”

Section: Resultsmentioning

confidence: 99%

“…A number of reported variations in the Nrat1 coding region affect transport activity ( Li et al, 2014 ; Xia et al, 2014 ; Lu et al, 2017 ) but not selectivity of Nrat1. We reasoned that careful examination of the metal binding site may facilitate to understand the selectivity of the transporter.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, OsNrat1, an Nramp member, was reported to specifically transport Al 3+ but not divalent metal ions such as Fe 2+ , Mn 2+ , and Cd 2+ , and required for Al tolerance in rice ( Xia et al, 2010 ). In sorghum, SbNrat1, a close homolog of rice OsNrat1, also was shown to selectively transport Al 3+ ( Lu et al, 2017 ). However, the molecular mechanisms underlying the Al transport selectivity of Nrat1 remain unknown.…”

Section: Introductionmentioning

confidence: 99%

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Bioinformatic and Functional Analysis of a Key Determinant Underlying the Substrate Selectivity of the Al Transporter, Nrat1

Yang

Liu

et al. 2018

Front. Plant Sci.

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Nrat1 is a member of the natural resistance-associated macrophage protein (Nramp) family of metal ion transporters in all organisms. Different from other Nramp members capable of transporting divalent metals, Nrat1 specifically transports trivalent aluminum (Al) ion. However, molecular mechanism underlying the Al transport selectivity of Nrat1 remains unknown. Here, we performed structure-function analyses of Nrat1 and other Nramp members to gain insights into the determinants of ion selectivity. A phylogenetic analysis showed that plant Nramp transporters could be divided into five groups. OsNrat1 was found in one of the individual clades and clustered with SbNrat1 and ZmNrat1 on the evolutionary tree. Structural modeling revealed that Nrat1 transporters adopted a common LeuT fold shared by many Nramp-family transporters that likely employed an identical transport mechanism. Sequence alignment and evolutionary conservation analysis of amino acids identified a metal-permeation pathway of Nrat1 centered at the metal binding site. The metal binding site of Nrat1 was characterized by two conserved sequence motifs, i.e., the Asp-Pro-Ser-Asn motif (motif A) and the Ala-Ile-Ile-Thr motif (motif B). Replacement of the Ala-Met-Val-Met motif B of the OsNramp3 manganese (Mn) transporter to that of Nrat1 resulted in a partial gain of Al transport activity and a total loss of Mn in yeast. Conversely, substitution of the motif B of OsNrat1 with that of OsNramp3 altered the Al transport activity. These observations indicated the metal binding site, particularly the motif B, as a key determinant of Al selectivity of Nrat1.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bioinformatic and Functional Analysis of a Key Determinant Underlying the Substrate Selectivity of the Al Transporter, Nrat1

Yang

Liu

et al. 2018

Front. Plant Sci.

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“…Overexpression of OsNrat1 enhances Al tolerance in Arabidopsis [101]. In other plants, OsNrat1 homologous genes, including ZmNrat1 [205] and SbNrat1 [206], have also been reported to be involved in transporting Al. To the best of our knowledge, the co-genetic transformation of Al transporters in the PM and tonoplast has not been reported.…”

Section: Transgenic Approaches Manipulating Al-tolerant Genesmentioning

confidence: 99%

Recent Advances in Understanding Mechanisms of Plant Tolerance and Response to Aluminum Toxicity

et al. 2021

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Aluminum (Al) toxicity is a major environmental stress that inhibits plant growth and development. There has been impressive progress in recent years that has greatly increased our understanding of the nature of Al toxicity and its mechanisms of tolerance. This review describes the transcription factors (TFs) and plant hormones involved in the adaptation to Al stress. In particular, it discusses strategies to confer plant resistance to Al stress, such as transgenic breeding, as well as small molecules and plant growth-promoting rhizobacteria (PGPRs) to alleviate Al toxicity. This paper provides a theoretical basis for the enhancement of plant production in acidic soils.

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“…Both mechanisms are controlled by the expression of a series of genes. In recent decades, many genes related to Al tolerance have been identified, such as organic acid transporter genes ( ALMTs and MATEs ) ( Hoekenga et al., 2006 ; Furukawa et al., 2007 ; Liu et al., 2013 ), antioxidative stress-related genes ( Wu et al., 2017 ), also including those encoding aluminum transporter (Nramps and ABC transporter, aquaporins) ( Huang et al., 2012 ; Li et al., 2014 ; Kochian et al., 2015 ; Lu et al., 2017 ; Wang et al., 2017 ), enzymes related to cell wall polysaccharide metabolism (XTHs) ( Zhu et al., 2012 ; Zhu et al., 2014 ), and transcription factor (STOP, WRKY, ASR, NAC) ( Iuchi et al., 2007 ; Ding et al., 2013 ; Arenhart et al., 2016 ; Li et al., 2018 ; Lou et al., 2019 ).…”

Section: Introductionmentioning

confidence: 99%

A Maize ZmAT6 Gene Confers Aluminum Tolerance via Reactive Oxygen Species Scavenging

Huang

et al. 2020

Front. Plant Sci.

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Aluminum (Al) toxicity is the primary limiting factor that affects crop yields in acid soil. However, the genes that contribute to the Al tolerance process in maize are still poorly understood. Previous studies have predicted that ZmAT6 is a novel protein which could be upregulated under Al stress condition. Here, we found that ZmAT6 is expressed in many tissues and organs and can be dramatically induced by Al in both the roots and shoots but particularly in the shoots. The overexpression of ZmAT6 in maize and Arabidopsis plants increased their root growth and reduced the accumulation of Al, suggesting the contribution of ZmAT6 to Al tolerance. Moreover, the ZmAT6 transgenic maize plants had lower contents of malondialdehyde and reactive oxygen species (ROS), but much higher proline content and even lower Evans blue absorption in the roots compared with the wild type. Furthermore, the activity of several enzymes of the antioxidant system, such as peroxidase (POD), superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX), increased in ZmAT6 transgenic maize plants, particularly SOD. Consistently, the expression of ZmSOD in transgenic maize was predominant upregulated by Al stress. Taken together, these findings revealed that ZmAT6 could at least partially confer enhanced tolerance to Al toxicity by scavenging ROS in maize.

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Functional characterization of the SbNrat1 gene in sorghum

Cited by 18 publications

References 25 publications

Bioinformatic and Functional Analysis of a Key Determinant Underlying the Substrate Selectivity of the Al Transporter, Nrat1

Bioinformatic and Functional Analysis of a Key Determinant Underlying the Substrate Selectivity of the Al Transporter, Nrat1

Recent Advances in Understanding Mechanisms of Plant Tolerance and Response to Aluminum Toxicity

A Maize ZmAT6 Gene Confers Aluminum Tolerance via Reactive Oxygen Species Scavenging

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