Functional characterization of the repeated UASINO element in the promoters of the INO1 and CHO2 genes of yeast

Koipally, Joseph; Ashburner, Brian P.; Bachhawat, Nandita; Gill, Tina; Hung, George; Henry, Susan A.; Lopes, John M.

doi:10.1002/(sici)1097-0061(19960615)12:7<653::aid-yea953>3.0.co;2-t

Cited by 31 publications

(24 citation statements)

References 19 publications

(14 reference statements)

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“…Several genes involved in fatty acid and sterol biosynthesis as well as inositol transport have been reported as containing putative UAS INO elements in their promoters (Greenberg and Lopes, 1996). Functional analyses of many of these genes confirm a role for Ino2p and/or UAS INO in their activation (Chirala et al, 1994;Koipally et al, 1996;Grauslund et al, 1999). Our microarray analysis of p180-expressing strains revealed several upregulated genes whose promoters contain known of putative UAS INO elements including INO1, GUT1, and SCS3 (unpublished results).…”

Section: Discussionsupporting

confidence: 52%

“…When inositol and choline levels are low, a transcription factor complex composed of the basic helix-loop-helix (bHLH) proteins, Ino2p and Ino4p, activates the expression of many genes encoding phospholipid, fatty acid, and sterol biosynthetic enzymes. Ino2p and Ino4p form a functional heterodimer that binds to a conserved upstream activating sequence (UAS INO ) residing in the promoters of these genes (Lopes et al, 1991;Ambroziak and Henry, 1994;Nikoloff and Henry, 1994;Koipally et al, 1996). Ino2p has been shown to contain transactivation domains (Schwank et al, 1995), whereas Ino4p is required for the binding of the complex to UAS INO .…”

Section: Introductionmentioning

confidence: 99%

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IN02, A Positive Regulator of Lipid Biosynthesis, Is Essential for the Formation of Inducible Membranes in Yeast

Block-Alper

Webster

Zhou

et al. 2002

MBoC

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Expression of the 180-kDa canine ribosome receptor inSaccharomyces cerevisiae leads to the accumulation of ER-like membranes. Gene expression patterns in strains expressing various forms of p180, each of which gives rise to unique membrane morphologies, were surveyed by microarray analysis. Several genes whose products regulate phospholipid biosynthesis were determined by Northern blotting to be differentially expressed in all strains that undergo membrane proliferation. Of these, the INO2 gene product was found to be essential for formation of p180-inducible membranes. Expression of p180 in ino2Δ cells failed to give rise to the p180-induced membrane proliferation seen in wild-type cells, whereas p180 expression in ino4Δ cells gave rise to membranes indistinguishable from wild type. Thus, Ino2p is required for the formation of p180-induced membranes and, in this case, appears to be functional in the absence of its putative binding partner, Ino4p.

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Section: Discussionsupporting

confidence: 52%

Section: Introductionmentioning

confidence: 99%

IN02, A Positive Regulator of Lipid Biosynthesis, Is Essential for the Formation of Inducible Membranes in Yeast

Block-Alper

Webster

Zhou

et al. 2002

MBoC

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“…Confirmation of a requirement for increased lipid biosynthesis comes from published studies in yeast which demonstrated that p180-induced membrane biogenesis fails to occur in cells lacking the INO2 gene, a master regulator of lipid biosynthesis . Virtually all of the lipid biosynthetic gene sequences that have been examined have INO2 regulatory elements in their promoter sequences (Lopes and Henry 1991;Nikoloff et al, 1992;Ambroziak and Henry, 1994;Nikoloff and Henry, 1994;Koipally et al, 1996). This group includes a multitude of enzymes and transporters involved in lipid and sterol metabolism (Greenberg and Lopes, 1996;Henry and PattonVogt, 1998).…”

Section: Discussionmentioning

confidence: 99%

Knockdown of p180 Eliminates the Terminal Differentiation of a Secretory Cell Line

Benyamini

Webster

Meyer

2009

MBoC

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We have previously reported that the expression in yeast of an integral membrane protein (p180) of the endoplasmic reticulum (ER), isolated for its ability to mediate ribosome binding, is capable of inducing new membrane biogenesis and an increase in secretory capacity. To demonstrate that p180 is necessary and sufficient for terminal differentiation and acquisition of a secretory phenotype in mammalian cells, we studied the differentiation of a secretory cell line where p180 levels had been significantly reduced using RNAi technology and by transiently expressing p180 in nonsecretory cells. A human monocytic (THP-1) cell line, that can acquire macrophage-like properties, failed to proliferate rough ER when p180 levels were lowered. The Golgi compartment and the secretion of apolipoprotein E (Apo E) were dramatically affected in cells expressing reduced p180 levels. On the other hand, expression of p180 in a human embryonic kidney nonsecretory cell line (HEK293) showed a significant increase in proliferation of rough ER membranes and Golgi complexes. The results obtained from knockdown and overexpression experiments demonstrate that p180 is both necessary and sufficient to induce a secretory phenotype in mammalian cells. These findings support a central role for p180 in the terminal differentiation of secretory cells and tissues. INTRODUCTIONAt the morphological level, the development of secretory cells and tissues has been well documented. From a molecular point of view, details of how cells acquire the ability to secrete at high levels as part of the process of their terminal differentiation are less well understood. Early in mammalian embryogenesis, the cytoplasm of cells of exocrine tissues, such as pancreas or liver, possesses a mere fraction of the intracellular organelles that they ultimately acquire upon terminal differentiation (Dallner et al., 1966a,b;Pictet et al., 1972;Slack, 1995;Debas, 1997). As differentiation of these tissues progresses, their cells take on a morphology that is dominated by membranous elements of the secretory pathway, most notably rough endoplasmic reticulum (ER) (Jamieson and Palade, 1967a,b). A similar situation exists in the immune system, in which B-lymphocytes differentiate into plasma cells as the result of being triggered by the appropriate antigen Shohat et al., 1973;Wiest et al., 1989Wiest et al., , 1990Chen-Kiang 1995;Gass et al., 2002;Iwakoshi et al., 2003;Federovitch et al., 2005;Fagone et al., 2007). A prerequisite for achieving such a "secretory phenotype" is intracellular membrane proliferation, which is in turn needed to expand the cell's capacity to synthesize, process, transport, and ultimately release peptides and proteins into the extracellular space.Limited success has been achieved, in a few cell types, in recapitulating membrane proliferation reminiscent of secretory cell differentiation. Through the ectopic expression of membrane proteins, ER-like membranes have been produced in yeast as well as in various mammalian cells (Wright et al., 1988;Vergeres et ...

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“…In their presence, transcription is repressed and in their absence, it is activated. This regulation is controlled by the cis-acting promoter element UAS INO [9,10], which is the binding site for the heterodimeric transcriptional activator, Ino2p/Ino4p [11,12]. Despite the constitutive binding of Ino2p/Ino4p to UAS INO [13,14], inositol suppresses transcriptional activation via Opi1p, which mediates repression through its activator interaction domain, by binding to the repressor interaction domain of Ino2p [15].…”

Section: Introductionmentioning

confidence: 99%

Activator-dependent recruitment of SWI/SNF and INO80 during INO1 activation

Ford¹,

Odeyale²,

Shen³

2008

Biochemical and Biophysical Research Communications

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Transcriptional activation of yeast INO1 requires SWI/SNF and INO80 for nucleosome disruption at the promoter. However, the cooperative interplay among remodelers and their recruitment dynamics in activation have thus far been vague. Here, we showed, using chromatin immunoprecipitation, that both SWI/SNF and INO80 are present at the promoter and are restricted to the promoter, indicating that they directly participate in localized INO1 chromatin remodeling. Furthermore, both SWI/SNF and INO80 are absent at the INO1 promoter in ino2Δ cells, suggesting that these are activator-dependent remodelers. We have also found that the presence of INO80 is required for SWI/SNF recruitment, indicating that INO80 arrives first at the promoter followed by SWI/SNF. In light of these findings, we proposed a model which describes the order of events in INO1 activation.

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Functional characterization of the repeated UASINO element in the promoters of the INO1 and CHO2 genes of yeast

Cited by 31 publications

References 19 publications

IN02, A Positive Regulator of Lipid Biosynthesis, Is Essential for the Formation of Inducible Membranes in Yeast

IN02, A Positive Regulator of Lipid Biosynthesis, Is Essential for the Formation of Inducible Membranes in Yeast

Knockdown of p180 Eliminates the Terminal Differentiation of a Secretory Cell Line

Activator-dependent recruitment of SWI/SNF and INO80 during INO1 activation

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