Functional characterization of acetylcholine receptors and calcium signaling in rat testicular capsule contraction

Silva, Edilson Dantas da; Souza, Bruno Palmieri de; Rodrigues, Juliano Quintella Dantas; Caricati‐Neto, Afonso; Jurkiewicz, Aron; Jurkiewicz, Neide Hyppolito

doi:10.1016/j.ejphar.2013.07.007

Cited by 7 publications

(5 citation statements)

References 40 publications

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“…Interestingly, in isolated rat uterus, relaxin plays a double role as a transporter and buffer of Ca 2+ (Fields 2005). For contraction of the rat testicular capsule, Ca 2+ is needed (da Silva Júnior et al 2013). Hence, Ca 2+ , together with relaxin, of which the contractile properties are well-known, is an important player controlling the interstitium tonus and creating the interstitial microenvironment.…”

Section: Discussionmentioning

confidence: 99%

Telocytes in the mouse testicular interstitium: implications of G-protein-coupled estrogen receptor (GPER) and estrogen-related receptor (ERR) in the regulation of mouse testicular interstitial cells

et al. 2018

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Telocytes (TCs), a novel type of interstitial cells, are involved in tissue homeostasis maintenance. This study aimed to investigate TC presence in the interstitium of mouse testis. Additionally, inactivation of the G-coupled membrane estrogen receptor (GPER) in the testis was performed to obtain insight into TC function, regulation, and interaction with other interstitial cells. Mice were injected with a GPER antagonist (G-15; 50 μg/kg bw), and the GPER-signaling effect on TC distribution, ultrastructure, and function, as well as the interstitial tissue interaction of GPER with estrogen-related receptors (ERRs), was examined. Microscopic observations of TC morphology were performed with the use of scanning and transmission electron microscopes. Telocyte functional markers (CD34; c-kit; platelet-derived growth factor receptors α and β, PDGFRα and β; vascular endothelial growth factor, VEGF; and vimentin) were analyzed by immunohistochemistry/immunofluorescence and Western blot. mRNA expression of CD34 as well as ERR α, β, and γ was measured by qRT-PCR. Relaxin and Ca concentrations were analyzed by immunoenzymatic and colorimetric assays, respectively. For the first time, we reveal the presence of TCs in the interstitium together with the peritubular area of mouse testis. Telocytes were characterized by specific features such as a small cell body and extremely long prolongations, constituting a three-dimensional network mainly around the interstitial cells. Expression of all TC protein markers was confirmed. Based on scanning electron microscopic observation in GPER-blocked testis, groups of TCs were frequently seen. No changes were found in TC ultrastructure in GPER-blocked testis when compared to the control. However, tendency to TC number change (increase) after the blockage was observed. Concomitantly, no changes in mRNA CD34 expression and increase in ERR expression were detected in GPER-blocked testes. In addition, Ca was unchanged; however, an increase in relaxin concentration was observed. Telocytes are an important component of the mouse testicular interstitium, possibly taking part in maintaining its microenvironment as well as contractile and secretory functions (via themselves or via controlling of other interstitial cells). These cells should be considered a unique and useful target cell type for the prevention and treatment of testicular interstitial tissue disorders based on estrogen-signaling disturbances.

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Section: Discussionmentioning

confidence: 99%

Telocytes in the mouse testicular interstitium: implications of G-protein-coupled estrogen receptor (GPER) and estrogen-related receptor (ERR) in the regulation of mouse testicular interstitial cells

et al. 2018

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“…The motor activity of mammalian testicular capsule contributes to male fertility and infertility, but the acetylcholine receptors are related to the contractions . The cholinergic system is not restricted to neurons but plays an important role in the germ cell differentiation .…”

Section: Discussionmentioning

confidence: 99%

Ellagic acid and rosmarinic acid attenuate doxorubicin-induced testicular injury in rats

Georgy

Maher²

2017

J Biochem Mol Toxicol

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The anticancer drug doxorubicin causes testicular toxicity as an undesirable effect. The present study was undertaken to investigate the possible protection of ellagic acid and rosmarinic acid during doxorubicin administration. For this purpose eight groups of male Sprague-Dawley rats were used (n = 10), one group received vehicle served as control, and other groups received 5 mg/kg doxorubicin twice a week for 2 weeks for a cumulative dose of 20 mg/kg, ellagic acid (10 mg/kg/day, 14 consecutive days p.o.), rosmarinic acid (75 mg/kg/day, 14 consecutive days p.o.), ellagic acid and rosmarinic acid. The latter three regimens were given to control and doxorubicin-received rats. Doxorubicin decreased testicular relative weight, sperm count, motility, serum testosterone, testicular glycogen, and sialic acid with increased incidence of histopathological changes, oxidative stress, tumor necrosis factor-alpha, as well as cholinesterase activity. Conversely, ellagic and rosmarinic acid treatment ameliorated such damage, thus showing the possibility to use as an adjuvant during doxorubicin treatment.

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“…Alternatively, smoking has a negative association with male fertility [ 46 ]. Cholinergic signaling plays a role in testes cells [ 47 ], in particular the contraction of smooth muscle in the testicular capsule that propels sperm from the seminiferous tubules to the epididymis; contractile dysfunctions in the testicular capsule are implicated in male infertility [ 48 ]. This and other testicular functions of acetylcholine might be disrupted by nicotine, which is a cholinergic toxin.…”

Section: Discussionmentioning

confidence: 99%

The low prevalence of female smoking in the developing world: gender inequality or maternal adaptations for fetal protection?

Hagen

Garfield

Sullivan

2016

EMPH

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Background: Female smoking prevalence is dramatically lower in developing countries (3.1%) than developed countries (17.2%), whereas male smoking is similar (32% vs 30.1%). Low female smoking has been linked to high gender inequality. Alternatively, to protect their offspring from teratogenic substances, pregnant and lactating women appear to have evolved aversions to toxic plant substances like nicotine, which are reinforced by cultural proscriptions. Higher total fertility rates (TFRs) in developing countries could therefore explain their lower prevalence of female smoking.Objective: To compare the associations of TFR and gender inequality with national prevalence rates of female and male smoking.Methods: Data from a previous study of smoking prevalence vs gender inequality in 74 countries were reanalysed with a regression model that also included TFR. We replicated this analysis with three additional measures of gender equality and 2012 smoking data from 173 countries.Results: A 1 SD increase in TFR predicted a decrease in female smoking prevalence by factors of 0.58–0.77, adjusting for covariates. TFR had a smaller and unexpected negative association with male smoking prevalence. Increased gender equality was associated with increased female smoking prevalence, and, unexpectedly, with decreased male smoking prevalence. TFR was also associated with an increase in smoking prevalence among postmenopausal women.Conclusions: High TFR and gender inequality both predict reduced prevalence of female smoking across nations. In countries with high TFR, adaptations and cultural norms that protect fetuses from plant toxins might suppress smoking among frequently pregnant and lactating women.

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Functional characterization of acetylcholine receptors and calcium signaling in rat testicular capsule contraction

Cited by 7 publications

References 40 publications

Telocytes in the mouse testicular interstitium: implications of G-protein-coupled estrogen receptor (GPER) and estrogen-related receptor (ERR) in the regulation of mouse testicular interstitial cells

Telocytes in the mouse testicular interstitium: implications of G-protein-coupled estrogen receptor (GPER) and estrogen-related receptor (ERR) in the regulation of mouse testicular interstitial cells

Ellagic acid and rosmarinic acid attenuate doxorubicin-induced testicular injury in rats

The low prevalence of female smoking in the developing world: gender inequality or maternal adaptations for fetal protection?

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