Functional and Phenotypic Characteristics of Alternative Activation Induced in Human Monocytes by Interleukin-4 or the Parasitic Nematode Brugia malayi

Semnani, Roshanak Tolouei; Mahapatra, Lily; Moore, Vanessa; Sanprasert, Vivornpun; Nutman, Thomas B.

doi:10.1128/iai.05191-11

Cited by 51 publications

(65 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In fact, human monocytes can be activated using either LPS/IFN-␥ or IL-4/IL-13 to promote development of M1-or M2-type cells, respectively (16,(24)(25)(26). While M1 macrophages produce an array of inflammatory cytokines, including IL-1␣, IL-1␤, TNF-␣, and IL-6, M2 macrophages in humans can be distinguished by their production of a set of chemokines, including CCL13, CCL18, and CCL22 (12,26).…”

Section: Discussionmentioning

confidence: 99%

“…Tissue macrophages can also be subdivided into at least three populations: M1, characterized by an inflammatory profile induced primarily during a Th1 response; M2, which have both anti-inflammatory and tissue repair functions (9), largely driven by IL-4 and/or IL-13; and finally, regulatory macrophages (Mreg) that are considered to be the primary source of innate . Although there is increasing evidence indicating human monocytes can be polarized in vitro with LPS and gamma interferon (IFN-␥) into M1, with IL-4/IL-13 into M2, or with a combination of immune complexes and Toll-like receptor (TLR) ligands into Mreg (15,16), little is known about the ontogeny of these subsets.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Human Monocyte Subsets at Homeostasis and Their Perturbation in Numbers and Function in Filarial Infection

et al. 2014

Self Cite

View full text Add to dashboard Cite

To characterize the function and plasticity of the major human circulating monocyte populations and to explore their role in systemic helminth infection, highly purified (by flow-based sorting) human monocyte subsets [nonclassical]) were examined at homeostasis and after activation. Among these three subsets the classical and intermediate subsets were found to be the major sources of inflammatory and regulatory cytokines, as well as cytokines/chemokines associated with alternative activation, whereas the nonclassical and classical populations demonstrated an ability to transmigrate through endothelial monolayers. Moreover, it was primarily the classical subset that was the most efficient in promoting autologous T cell proliferation. The distribution of these subsets changed in the context of a systemic helminth (Wuchereria bancrofti) infection such that patent infection altered the frequency and distribution of these monocyte subsets with the nonclassical monocytes being expanded (almost 2-fold) in filarial infection. To understand further the filarial/monocyte interface, in vitro modeling demonstrated that the classical subset internalized filarial antigens more efficiently than the other two subsets but that the parasite-driven regulatory cytokine interleukin-10 was exclusively coming from the intermediate subset. Our data suggest that monocyte subsets have a differential function at homeostasis and in response to helminth parasites.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Human Monocyte Subsets at Homeostasis and Their Perturbation in Numbers and Function in Filarial Infection

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…Furthermore, we have done extensive in vitro studies showing that M f s need not be differentiated prior to polarization with IL-4. In fact, short-term exposure of M f s to IL-4 is suf fi cient to induce the AAM F phenotype in human cells [ 42 ] . In addition, the mf stage of B. malayi or soluble factors from mf induce a programmed response very similar to that induced by IL-4, resulting in upregulation of MRC1 or CD206, CCL15, CCL17, CCL18, CCL22, CD273, and CD274.…”

Section: Humansmentioning

confidence: 99%

The Interaction Between Filarial Parasites and Human Monocyte/Macrophage Populations

Semnani

2013

Crossroads Between Innate and Adaptive Immunity IV

Self Cite

View full text Add to dashboard Cite

Lymphatic filariasis is a mafor tropical disease affecting approximately 120 million people worldwide. Patent infection, by and large, is clinically asymptomatic but is associated with the inability of T cells to proliferate or produce IFN-γ in response to parasite antigen. Monocyte dysfunction is one hypothesis felt to explain the lack of an antigen-specific T cell response. In fact, monocytes from filaria-infected individuals have been shown to be studded with internalized filarial antigens. Understanding how the phenotype and the function of these monocytes are altered through the internalization of these parasite antigens is one of the areas our laboratory has focused on. In fact, the existence and/or function of alternatively activated macrophages in murine models of filarial infections have been extensively studied. Whether this population of macrophages can be induced in human filarial infections is the main focus of this review.

show abstract

“…Previous studies 8 have shown that CLEC10a mRNA expression is increased on M2 MDM and there have been reports of increases on macrophages in a number of diseases [43][44][45] . However, to our knowledge there have been no studies of both mRNA and protein expression.…”

Section: Europe Pmc Funders Author Manuscriptsmentioning

confidence: 99%

Phenotypic Characterisation Of Lung Macrophages In Asthma: Over-Expression Of CCL17

Staples¹,

Hinks²,

Ward³

et al. 2012

A35. Recent Advances in Phagocyte Biology

View full text Add to dashboard Cite

show abstract

Functional and Phenotypic Characteristics of Alternative Activation Induced in Human Monocytes by Interleukin-4 or the Parasitic Nematode Brugia malayi

Cited by 51 publications

References 52 publications

Human Monocyte Subsets at Homeostasis and Their Perturbation in Numbers and Function in Filarial Infection

Human Monocyte Subsets at Homeostasis and Their Perturbation in Numbers and Function in Filarial Infection

The Interaction Between Filarial Parasites and Human Monocyte/Macrophage Populations

Phenotypic Characterisation Of Lung Macrophages In Asthma: Over-Expression Of CCL17

Contact Info

Product

Resources

About