Functional and Multifunctional Nanoparticles for Bioimaging and Biosensing

Selvan, Subramanian Tamil; Tan, Timothy Thatt Yang; Yi, Dong Kee; Jana, Nikhil R.

doi:10.1021/la903512m

Cited by 304 publications

(223 citation statements)

References 59 publications

(162 reference statements)

Supporting

Mentioning

218

Contrasting

Unclassified

Order By: Relevance

“…Several methods have been developed for the design of water-soluble QDs by using polymers, thiols ( Figure 3B) and silanization ( Figure 3C) [119,120]. Such coatings are not only required to improve the water solubility but also to equip the QD with functional groups, which in turn are needed for further modification and conjugation chemistry ( Figure 3B).…”

Section: Conjugation Approachesmentioning

confidence: 99%

Cell-penetrating peptides for nanomedicine – how to choose the right peptide

2015

View full text Add to dashboard Cite

More than two decades ago, a group of peptides, now known as cell-penetrating peptides, sparked the hope that the ultimate carrier molecules have been found. The high expectations for these peptides, which are reflected in their bold name, led to many disappointments due to the controversial results their utilization entailed and nowadays even their effectiveness has been called into question. In this review, we discuss the uptake mechanism and application of cell penetrating peptides as mediators for organelle specific delivery of nanocarriers, pointing out the possibilities as well as strategies of their successful utilization. Additionally, we provide an overview of the conjugation techniques usually employed for the attachment of cell penetrating peptides to quantum dots, as well as silver and gold nanoparticles, and we address the various aspects that need to be considered for the successful implementation of cell penetrating peptides for organelle-specific delivery of nanoparticles into cells.Keywords: cell penetrating peptide; drug delivery; gold nanoparticle; nanomedicine; polymeric nanoparticle; quantum dot. Highlights-We discuss the mechanisms of cell penetrating peptide's uptake and briefly introduce the different types of peptides in order to present a more complete picture of the intracellular fate of the peptide-cargo conjugates. -We highlight the recent applications of cell penetrating peptides in the targeted delivery of quantum dots as well as gold, silver and polymeric nanoparticles into mammalian cells in the context of organelle specificity. -We give a short overview of the conjugation techniques usually utilized for the attachment of cell penetrating peptides to quantum dots as well as silver and gold nanoparticles.

show abstract

Section: Conjugation Approachesmentioning

confidence: 99%

Cell-penetrating peptides for nanomedicine – how to choose the right peptide

2015

View full text Add to dashboard Cite

show abstract

“…More studies are required in order to assess the toxicological effects of NPs, such as alterations in the natural morphology or functions of the cell, prior to their approval for in vivo applications. For QD-based nanosystems (even coated with polymers or silica), the cytotoxicity may arise from the potential release of heavy metals (Lewinski et al, 2008;Selvan et al, 2010).…”

Section: Biodistribution and Biocompatibilitymentioning

confidence: 99%

Polymeric nanoparticles for optical sensing

Canfarotta

Whitcombe

Piletsky

2013

Biotechnology Advances

120

View full text Add to dashboard Cite

Nanotechnology is a powerful tool for use in diagnostic applications. For these purposes a variety of functional nanoparticles containing fluorescent labels, gold and quantum dots at their cores have been produced, with the aim of enhanced sensitivity and multiplexing capabilities. This work will review progress in the application of polymeric nanoparticles in optical diagnostics, both for in vitro and in vivo detection, together with a discussion of their biodistribution and biocompatibility.

show abstract

“…The silica-coating technique is used as a nanoplatform to make other novel hybrid nanoparticle architectures for in vitro and in vivo optical imaging applications. [31][32][33] Surface coating with silica shells yields less toxicity and high photostability, in addition to minimizing oxidation of the QD core. In addition, silica is an inert layer that confers water solubility and shields the optical property of the core.…”

Section: Preparation Of Bare Cds and Silica-coated Cdsmentioning

confidence: 99%

“…To attain high specificity, the silica-coated QDs were labeled with the CD31 antibody, an endothelial marker highly specific to vascular lineage cells. Although silica-coated cadmium selenide and cadmium telluride QDs have been previously documented and used for labeling cells, [31][32][33] the fabrication of silicacoated CdS QDs and their in vitro and in vivo bioimaging applications have been unreported and remain elusive to date. We propose the present synthesis methodology as an easy, economic method that does not need rigorous conditions or have complex requirements.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and application of luminescent single CdS quantum dot encapsulated silica nanoparticles directed for precision optical bioimaging

Veeranarayanan¹,

Poulose²,

Mohamed³

et al. 2012

IJN

View full text Add to dashboard Cite

This paper presents the synthesis of aqueous cadmium sulfide (CdS) quantum dots (QDs) and silica-encapsulated CdS QDs by reverse microemulsion method and utilized as targeted bio-optical probes. We report the role of CdS as an efficient cell tag with fluorescence on par with previously documented cadmium telluride and cadmium selenide QDs, which have been considered to impart high levels of toxicity. In this study, the toxicity of bare QDs was efficiently quenched by encapsulating them in a biocompatible coat of silica. The toxicity profile and uptake of bare CdS QDs and silica-coated QDs, along with the CD31-labeled, silica-coated CdS QDs on human umbilical vein endothelial cells and glioma cells, were investigated. The effect of size, along with the time-dependent cellular uptake of the nanomaterials, has also been emphasized. Enhanced, high-specificity imaging toward endothelial cell lines in comparison with glioma cells was achieved with CD31 antibody-conjugated nanoparticles. The silica-coated nanomaterials exhibited excellent biocompatibility and greater photostability inside live cells, in addition to possessing an extended shelf life. In vivo biocompatibility and localization study of silica-coated CdS QDs in medaka fish embryos, following direct nanoparticle exposure for 24 hours, authenticated the nanomaterials' high potential for in vivo imaging, augmented with superior biocompatibility. As expected, CdS QD-treated embryos showed 100% mortality, whereas the silica-coated QD-treated embryos stayed viable and healthy throughout and after the experiments, devoid of any deformities. We provide highly cogent and convincing evidence for such silica-coated QDs as a model nanoparticle in practice, to achieve in vitro and in vivo precision targeted imaging.

show abstract

Functional and Multifunctional Nanoparticles for Bioimaging and Biosensing

Cited by 304 publications

References 59 publications

Cell-penetrating peptides for nanomedicine – how to choose the right peptide

Cell-penetrating peptides for nanomedicine – how to choose the right peptide

Polymeric nanoparticles for optical sensing

Synthesis and application of luminescent single CdS quantum dot encapsulated silica nanoparticles directed for precision optical bioimaging

Contact Info

Product

Resources

About