Functional and Histological Evidence for the Targeted Therapy Using Biocompatible Polycaprolactone Beads and Autologous Myoblasts in a Dog Model of Fecal Incontinence

Oh, Heung Kwon; Lee, Hye Seung; Lee, Jin Ho; Oh, Se Heang; Lim, Jae‐Young; Ahn, Soyeon; Hwang, Jeoung Yeon; Kang, Sung Bum

doi:10.1097/dcr.0000000000000346

Cited by 27 publications

(44 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our study, the anal pressures were comparable with the other animal studies although there are slight variations—possibly due to different techniques (balloon vs. double‐triple‐lumen‐catheter, electrical stimulation vs. spontaneous contraction) and different size of the anal canal in different species 13, 16, 19. Salcedo et al stated that rat anal pressures tend to return to baseline after sphincterotomy at 4 weeks even without intervention, but this does not occur after pudendal nerve transection 42.…”

Section: Discussionsupporting

confidence: 83%

Functional Outcome of Human Adipose Stem Cell Injections in Rat Anal Sphincter Acute Injury Model

Kuismanen

Juntunen

Narra

et al. 2018

Stem Cells Translational Medicine

View full text Add to dashboard Cite

Anal incontinence is a devastating condition that significantly reduces the quality of life. Our aim was to evaluate the effect of human adipose stem cell (hASC) injections in a rat model for anal sphincter injury, which is the main cause of anal incontinence in humans. Furthermore, we tested if the efficacy of hASCs could be improved by combining them with polyacrylamide hydrogel carrier, Bulkamid. Human ASCs derived from a female donor were culture expanded in DMEM/F12 supplemented with human platelet lysate. Female virgin Sprague‐Dawley rats were randomized into four groups (n = 14–15/group): hASCs in saline or Bulkamid (3 × 105/60 μl) and saline or Bulkamid without cells. Anorectal manometry (ARM) was performed before anal sphincter injury, at two (n = 58) and at four weeks after (n = 33). Additionally, the anal sphincter tissue was examined by micro‐computed tomography (μCT) and the histological parameters were compared between the groups. The median resting and peak pressure during spontaneous contraction measured by ARM were significantly higher in hASC treatment groups compared with the control groups without hASCs. There was no statistical difference in functional results between the hASC‐carrier groups (saline vs. Bulkamid). No difference was detected in the sphincter muscle continuation between the groups in the histology and μCT analysis. More inflammation was discovered in the group receiving saline with hASC. The hASC injection therapy with both saline and Bulkamid is a promising nonsurgical treatment for acute anal sphincter injury. Traditional histology combined with the 3D μCT image data lends greater confidence in assessing muscle healing and continuity. Stem Cells Translational Medicine 2018;7:295–304

show abstract

Section: Discussionsupporting

confidence: 83%

Functional Outcome of Human Adipose Stem Cell Injections in Rat Anal Sphincter Acute Injury Model

Kuismanen

Juntunen

Narra

et al. 2018

Stem Cells Translational Medicine

View full text Add to dashboard Cite

show abstract

“…In another study, myogenic stem cells improved external sphincter function after transection of both the internal and external sphincter only when injected along with a biogel scaffold 2 weeks after sphincter damage . Other studies conducted in dog models of FI with internal and external sphincter injury have also injected myoblasts incorporated in polycaprolactone beads . The resting and contractile pressures were improved.…”

Section: Discussionmentioning

confidence: 98%

Successful Treatment of Passive Fecal Incontinence in an Animal Model Using Engineered Biosphincters: A 3-Month Follow-Up Study

Bohl

Zakhem

Bitar

2017

Stem Cells Translational Medicine

View full text Add to dashboard Cite

Fecal incontinence (FI) is the involuntary passage of fecal material. Current treatments have limited successful outcomes. The objective of this study was to develop a large animal model of passive FI and to demonstrate sustained restoration of fecal continence using anorectal manometry in this model after implantation of engineered autologous internal anal sphincter (IAS) biosphincters. Twenty female rabbits were used in this study. The animals were divided into three groups: (a) Non‐treated group: Rabbits underwent IAS injury by hemi‐sphincterectomy without treatment. (b) Treated group: Rabbits underwent IAS injury by hemi‐sphincterectomy followed by implantation of autologous biosphincters. (c) Sham group: Rabbits underwent IAS injury by hemi‐sphincterectomy followed by re‐accessing the surgical site followed by immediate closure without implantation of biosphincters. Anorectal manometry was used to measure resting anal pressure and recto‐anal inhibitory reflex (RAIR) at baseline, 1 month post‐sphincterectomy, up to 3 months after implantation and post‐sham. Following sphincterectomy, all rabbits had decreased basal tone and loss of RAIR, indicative of FI. Anal hygiene was also lost in the rabbits. Decreases in basal tone and RAIR were sustained more than 3 months in the non‐treated group. Autologous biosphincters were successfully implanted into eight donor rabbits in the treated group. Basal tone and RAIR were restored at 3 months following biosphincter implantation and were significantly higher compared with rabbits in the non‐treated and sham groups. Histologically, smooth muscle reconstruction and continuity was restored in the treated group compared with the non‐treated group. Results in this study provided promising outcomes for treatment of FI. Results demonstrated the feasibility of developing and validating a large animal model of passive FI. This study also showed the efficacy of the engineered biosphincters to restore fecal continence as demonstrated by manometry. Stem Cells Translational Medicine 2017;6:1795–1802

show abstract

“…The durability of the typical PCL polymer varies between two and four years, which sometimes is not suitable for certain applications like tissue engineering where a shorter degradation time is required. To shorten the degradation of PCL, other biodegradable polymers are often incorporated or copolymerized with it [11,12].…”

Section: Introductionmentioning

confidence: 99%

Effect of Polymer Concentration, Rotational Speed, and Solvent Mixture on Fiber Formation Using Forcespinning®

Obregon

Agubra

Pokhrel

et al. 2016

Fibers

View full text Add to dashboard Cite

Polycaprolactone (PCL) fibers were produced using Forcespinning ® (FS). The effects of PCL concentration, solvent mixture, and the spinneret rotational speed on fiber formation were evaluated. The concentration of the polymer in the solvents was a critical determinant of the solution viscosity. Lower PCL concentrations resulted in low solution viscosities with a correspondingly low fiber production rate with many beads. Bead-free fibers with high production rate and uniform fiber diameter distribution were obtained from the optimum PCL concentration (i.e., 12.5 wt%) with tetrahydrofuran (THF) as the solvent. The addition of N, N-dimethylformamide (DMF) to the THF solvent promoted the gradual formation of beads, split fibers, and generally affected the distribution of fiber diameters. The crystallinity of PCL fibers was also affected by the processing conditions, spinning speed, and solvent mixture.

show abstract

Functional and Histological Evidence for the Targeted Therapy Using Biocompatible Polycaprolactone Beads and Autologous Myoblasts in a Dog Model of Fecal Incontinence

Abstract: This study shows that an injection of polycaprolactone beads containing autologous myoblasts may improve anal sphincter function in an animal model of fecal incontinence.

Cited by 27 publications

References 32 publications

Functional Outcome of Human Adipose Stem Cell Injections in Rat Anal Sphincter Acute Injury Model

Functional Outcome of Human Adipose Stem Cell Injections in Rat Anal Sphincter Acute Injury Model

Successful Treatment of Passive Fecal Incontinence in an Animal Model Using Engineered Biosphincters: A 3-Month Follow-Up Study

Effect of Polymer Concentration, Rotational Speed, and Solvent Mixture on Fiber Formation Using Forcespinning®

Contact Info

Product

Resources

About