2007
DOI: 10.1111/j.1467-7652.2007.00288.x
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Abstract: SummaryVirus-induced gene silencing (VIGS) is a powerful tool for functional analysis of genes in plants. A wide-host-range VIGS vector, which was developed based on the Cucumber mosaic virus (CMV), was tested for its ability to silence endogenous genes involved in flavonoid biosynthesis in soybean. Symptomless infection was established using a pseudorecombinant virus, which enabled detection of specific changes in metabolite content by VIGS. It has been demonstrated that the yellow seed coat phenotype of vari… Show more

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Cited by 128 publications
(116 citation statements)
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References 81 publications
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“…Quantitative RT-PCR was done essentially as described previously (Nagamatsu et al 2007). The quantitative RT-PCR mixture was prepared by mixing a 1-ml aliquot of the reaction mixture of cDNA synthesis, 5 ml of 1.2 mm primer premix, 10 ml SYBR Premix ExTaq Perfect Real Time (TaKaRa Bio), and water to a final volume of 20 ml.…”
Section: Methodsmentioning
confidence: 99%
“…Quantitative RT-PCR was done essentially as described previously (Nagamatsu et al 2007). The quantitative RT-PCR mixture was prepared by mixing a 1-ml aliquot of the reaction mixture of cDNA synthesis, 5 ml of 1.2 mm primer premix, 10 ml SYBR Premix ExTaq Perfect Real Time (TaKaRa Bio), and water to a final volume of 20 ml.…”
Section: Methodsmentioning
confidence: 99%
“…Recently, the use of internal markers for VIGS based on pigment accumulation in petunia (Petunia hybrida), soybean (Glycine max), and tomato has proven very successful for empowering the method as a reverse genetics tool (Chen et al, 2004b;Nagamatsu et al, 2007;Spitzer et al, 2007;Jiang et al, 2008). Tomato introgression lines accumulating anthocyanins in their fruit have been described, but this accumulation is restricted to the epidermis; therefore, the use of these lines is restricted to analysis of gene function in this tissue.…”
mentioning
confidence: 99%
“…As a result of various recent research, several groups have reported successful VIGS in various plants such as cassava (Fofana et al, 2004), barley (Bruun-Rasmussen et al, 2007;Naylor et al, 2005), soybean (Nagamatsu et al, 2007), medicago and lathyrus (Gronlund et al, 2008). Recently, we have developed a vector for foreign gene cloning by manipulating RNA2 of Gn-CMV, which has a wide range of host plants and shows higher infectivity than Y-CMV in tomato and chili pepper, however, the virus vector composed of only Gn-CMV genomes showed VIGS symptoms with very low efficiency even in N. benthamiana (data not shown).…”
mentioning
confidence: 99%
“…Over the past 15 years, several RNA virus vectors have been developed for VIGS. These include Tobacco mosaic virus (Kumagai et al, 1995), Potato virus X (Ruiz et al, 1998), Tobacco rattle virus Ratcliff et al, 1999), Barley stripe mosaic virus (Holzverg, 2002), Pea early browning virus (Constantin et al, 2004), Bean pod mottle virus (Zhang and Ghabrial, 2006), Cucumber mosaic virus (Nagamatsu, 2007;Otagaki et al, 2006), and Pepper mottle mosaic virus . VIGS has many advantages over other methods, in which it is rapid and does not require a recalcitrant plant transformation (Burch-smith et al, 2004).…”
mentioning
confidence: 99%