Functional Amyloids in Pseudomonas aeruginosa Are Essential for the Proteome Modulation That Leads to Pathoadaptation in Pulmonary Niches

Beg, Ayesha Z.; Rashid, Faraz; Talat, Absar; Haseen, Mohd Azam; Raza, Nadeem; Akhtar, Kafil; Dueholm, Morten Simonsen; Khan, Asad U.

doi:10.1128/spectrum.03071-22

Cited by 6 publications

(1 citation statement)

References 92 publications

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“…S. mutans produces functional amyloids formed by antigen I/II polypeptides and other secreted proteins dependent on sortase-mediated assembly, which promote biofilm formation and are present in dental plaque ( Oli et al., 2012 ). Pseudomonas species can also produce a functional amyloid protein, encoded by the fap operon, that contributes to biofilm mechanical robustness ( Zeng et al., 2015 ) and promotes in vivo aggregation of P. aeruginosa in a rat model of lung infection ( Beg et al., 2022 ). Another extracellular protein that contributes to the structural stability of the P. aeruginosa biofilm matrix is CdrA, which is an adhesin belonging to a two-partner secretion system ( Borlee et al., 2010 ).…”

Section: Intrinsic and Extrinsic Factors Affecting Tissue-associated ...mentioning

confidence: 99%

Bacterial biofilms in the human body: prevalence and impacts on health and disease

Perry,

Tan

2023

Front. Cell. Infect. Microbiol.

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Bacterial biofilms can be found in most environments on our planet, and the human body is no exception. Consisting of microbial cells encased in a matrix of extracellular polymers, biofilms enable bacteria to sequester themselves in favorable niches, while also increasing their ability to resist numerous stresses and survive under hostile circumstances. In recent decades, biofilms have increasingly been recognized as a major contributor to the pathogenesis of chronic infections. However, biofilms also occur in or on certain tissues in healthy individuals, and their constituent species are not restricted to canonical pathogens. In this review, we discuss the evidence for where, when, and what types of biofilms occur in the human body, as well as the diverse ways in which they can impact host health under homeostatic and dysbiotic states.

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Section: Intrinsic and Extrinsic Factors Affecting Tissue-associated ...mentioning

confidence: 99%

Bacterial biofilms in the human body: prevalence and impacts on health and disease

Perry,

Tan

2023

Front. Cell. Infect. Microbiol.

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In vitro and in silico evaluation of the serrapeptase effect on biofilm and amyloids of Pseudomonas aeruginosa

Katsipis,

Avgoulas,

Geromichalos

et al. 2023

Appl Microbiol Biotechnol

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Pseudomonas aeruginosa is an emerging threat for hospitalized and cystic fibrosis patients. Biofilm, a microbial community embedded in extracellular polymeric substance, fortifies bacteria against the immune system. In biofilms, the expression of functional amyloids is linked with highly aggregative, multi-resistant strains, and chronic infections. Serrapeptase (SPT), a protease possessing similar or superior anti-microbial properties with many antibiotics, presents anti-amyloid potential. However, studies on the employment of SPT against Pseudomonas biofilms and Fap amyloid, or the possible mechanisms of action are scarce. Here, SPT inhibited biofilm formation of P. aeruginosa ATCC 27853 on both plastic and glass surfaces, with an IC50 of 11.26 µg/mL and 0.27 µg/mL, respectively. The inhibitory effect of SPT on biofilm was also verified with optical microscopy of crystal violet-stained biofilms and with confocal microscopy. Additionally, SPT caused a dose-dependent decrease of bacterial viability (IC50 of 3.07 µg/mL) as demonstrated by MTT assay. Reduction of bacterial functional amyloids was also demonstrated, employing both fluorescence microscopy with thioflavin T and photometrical determination of Congo-red-positive compounds. Both viability and functional amyloids correlated significantly with biofilm inhibition. Finally, in silico molecular docking studies provided a mechanistic insight into the interaction of SPT with FapC or FapD, proving that both peptides are possible targets of SPT. These results offer new insights into the biofilm formation of P. aeruginosa and potentiate the involvement of SPT in the prevention and eradication of Pseudomonas biofilms. Graphical abstract Key points • Serrapeptase inhibits biofilm formation of P. aeruginosa on plastic and glass. • Biofilm inhibition correlated with reduced viability and functional amyloid levels. • In silico studies indicated that serrapeptase may target FapC and FapD peptides.

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The past, present and future of polymicrobial infection research: Modelling, eavesdropping, terraforming and other stories

Bényei,

Nazeer,

Askenasy

et al. 2024

Advances in Microbial Physiology

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Functional Amyloids in Pseudomonas aeruginosa Are Essential for the Proteome Modulation That Leads to Pathoadaptation in Pulmonary Niches

Abstract: Pseudomonas aeruginosa is inextricably linked with chronic lung infections. In this study, the well-conserved Fap operon was found to be essential for pathoadaptation in pulmonary infection in a rat lung model.

Cited by 6 publications

References 92 publications

Bacterial biofilms in the human body: prevalence and impacts on health and disease

Bacterial biofilms in the human body: prevalence and impacts on health and disease

In vitro and in silico evaluation of the serrapeptase effect on biofilm and amyloids of Pseudomonas aeruginosa

The past, present and future of polymicrobial infection research: Modelling, eavesdropping, terraforming and other stories

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