Full-scale agricultural biogas plant metal content and process parameters in relation to bacterial and archaeal microbial communities over 2.5 year span

Repinc, Sabina Kolbl; Šket, Robert; Zavec, Domen; Mikuš, Katarina Vogel; Fermoso, Fernando G.; Stres, Blaž

doi:10.1016/j.jenvman.2018.02.058

Cited by 7 publications

(4 citation statements)

References 49 publications

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“…As shown in previous studies (De Vrieze et al, 2016;Heyer et al, 2013;Kirkegaard et al, 2017;Repinc et al, 2018), microbial fingerprints enable monitoring of the AD process' stability, and also single features of the fingerprinting methods correlate with process parameters. For example, the phylum Chloroflexi prefers the conditions in anaerobic digesters treating sewage sludge as feedstock (Bovio-Winkler et al, 2021;Speirs et al, 2019).…”

Section: Extending Our Knowledge About Microbiomes and Fingerprintsmentioning

confidence: 73%

Triangulation of microbial fingerprinting in anaerobic digestion reveals consistent fingerprinting profiles

Vrieze

Heyer

Props

et al. 2021

Preprint

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The anaerobic digestion microbiome has been puzzling us since the dawn of molecular methods for mixed microbial community analysis. Monitoring of the anaerobic digestion microbiome can either take place via a non-targeted holistic evaluation of the microbial community through fingerprinting or by targeted monitoring of selected taxa. Here, we compared four different microbial community fingerprinting methods, i.e., amplicon sequencing, metaproteomics, metabolomics and cytomics, in their ability to characterise the full-scale anaerobic digestion microbiome. Cytometric fingerprinting through cytomics reflects a, for anaerobic digestion, novel, single cell-based approach of direct microbial community fingerprinting by flow cytometry. Three different digester types, i.e., sludge digesters, digesters treating agro-industrial waste and dry anaerobic digesters, each reflected different operational parameters. The α-diversity analysis yielded inconsistent results, especially for richness, across the different methods. In contrast, β-diversity analysis resulted in comparable profiles, even when translated into phyla or functions, with clear separation of the three digester types. In-depth analysis of each method's features i.e., operational taxonomic units, metaproteins, metabolites, and cytometric traits, yielded certain similar features, yet, also some clear differences between the different methods, which was related to the complexity of the anaerobic digestion process. In conclusion, cytometric fingerprinting through flow cytometry is a reliable, fast method for holistic monitoring of the anaerobic digestion microbiome, and the complementary identification of key features through other methods could give rise to a direct interpretation of anaerobic digestion process performance.

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Section: Extending Our Knowledge About Microbiomes and Fingerprintsmentioning

confidence: 73%

Triangulation of microbial fingerprinting in anaerobic digestion reveals consistent fingerprinting profiles

Vrieze

Heyer

Props

et al. 2021

Preprint

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“…Slightly different enzymes have been considered as the initial step of process heterogeneity (Delvigne et al, 2014), the initiator of microbial diversity and process variability. This in turn results in ongoing redistribution of organic matter, trace elements and secondary metabolites between various microorganisms within a microbial community, generating fluctuations in the activity of the whole microbial community that ultimately results in a modified community structure over time, as reported before (Repinc et al, 2018;Sun et al, 2015).…”

Section: Introductionmentioning

confidence: 90%

“…Such cases force microorganisms to respond accordingly, increase their energetic expenditure and invest resources to fine-tune their protein makeup to their current environmental conditions. The lack of understanding of metabolic prerequisites of microorganisms in full-scale industrial anaerobic digesters may result in consequent borderline conditions with insufficient microbial activity towards methane production (Garuti et al, 2018;Murovec et al, 2018;Repinc et al, 2018).…”

Section: Perspectivesmentioning

confidence: 99%

“…Depending on the history, biogeography, and operational parameters of biogas reactors, crucial microbial constituents can fluctuate showing different abundance and level of activity as a consequence of, for instance, trace-element deficiency, long-term limitation of organic substrate or short-term overload (Murovec et al, 2018;Repinc et al, 2018;Sun et al, 2015). This variability builds up the diversity of both microorganisms and their enzymes implicated in the processes leading to methane and the complete mineralization of organic matter (i.e.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Trace element enzymes in reactions essential for anaerobic digestion

González¹,

Stres²

2019

Trace Elements in Anaerobic Biotechnologies

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Trace elements play a very important role on the performance and stability of biogas digesters from a variety of biomass-containing residues, both natural or synthetic. Degradation of these complex chemical compounds occurs by the interaction of numerous microorganisms carrying out a series of pathways involving fermentative processes that ultimately lead to methane production. The purpose of this study was to provide an overview of the direct relationships existing among trace elements and enzyme activity which regulates the anaerobic digestion processes carried out by these microorganisms. Methanogenesis is one of the most trace-element enriched enzymatic pathways in biology. Trace elements are major key elements in the functioning of multiple enzymes reviewed within this work. Although exact trace-element requirements may differ slightly between pathways depending on composition and the microorganisms involved, there are some general trends characterizing the anaerobic digestion processes. Iron (Fe) is the most abundantly required metal, followed by nickel (Ni), cobalt (Co), molybdenum (Mo), tungsten (W), and zinc (Zn). In order to sustain the

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An insight on the contributions of microbial communities and process parameters in enhancing biogas production

Nwokolo

Enebe

2022

Biomass Conv. Bioref.

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Full-scale agricultural biogas plant metal content and process parameters in relation to bacterial and archaeal microbial communities over 2.5 year span

Cited by 7 publications

References 49 publications

Triangulation of microbial fingerprinting in anaerobic digestion reveals consistent fingerprinting profiles

Triangulation of microbial fingerprinting in anaerobic digestion reveals consistent fingerprinting profiles

Trace element enzymes in reactions essential for anaerobic digestion

An insight on the contributions of microbial communities and process parameters in enhancing biogas production

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