Full genome sequence of Brevibacillus laterosporus strain B9, a biological control strain isolated from Zhejiang, China

Abstract

Brevibacillus laterosporus was newly classified from Bacillus laterosporus, which has ability to be used as a biological control agent in crop field. B. laterosporus strain B9 is an aerobic, motile, Gram-positive, spore-forming rod that was isolated from a field of Oryza sativa in Zhejiang, China in 2011. This bacterium has been confirmed to be a strong antagonist against bacterial brown strip of rice caused by Acidovorex avenae subsp. avenae. Here we describe the features of B. laterosporus strain B9, together with the complete genome sequence and its annotation. The 5,272,435bp genome contains 4804 protein-coding genes and 227 RNA-only encoding genes with 2 plasmids.

Structural Organization of Brevilaterin Biosynthesis in Brevibacillus laterosporus S62-9: A Novel MbtH-Independent Cationic Antimicrobial Peptide Synthetase System

“…Certainly, additional biosynthetic genes also played an important role in the formation of unique structures in this cluster (Table S3). For example, ORF 6 encoded an acetyltransferase that was 61.27% similar to the YdaF protein from Bacillus paralicheniformis B-14251, 30 ORF 11 was similar to a methyltransferase from B. laterosporus B9, 31 and ORF 35, which also encoded aminotransferase classes I and II, was similar to 8-amino-7-oxonononanoate synthase from B. laterosporus GI-9. 32 They helped in confirming the brevilaterin biosynthetic gene cluster's outer boundaries, although it was unclear how they were engaged in biosynthesis.…”

“…For example, ORF 6 encoded an acetyltransferase that was 61.27% similar to the YdaF protein from Bacillus paralicheniformis B-14251, ORF 11 was similar to a methyltransferase from B. laterosporus B9, and ORF 35, which also encoded aminotransferase classes I and II, was similar to 8-amino-7-oxonononanoate synthase from B. laterosporus GI-9 .…”

Spore surface proteins of Brevibacillus laterosporus are involved in insect pathogenesis

“…Interestingly, the genes encoding these proteins are shared with other B. laterosporus genomes so far sequenced122324. To date, no function has been associated with the two hypothetical proteins that we designated CpbA and CpbB, where Cpb stands for spore coat-parasporal body complex.…”

Bacteriocin-like protein produced Brevibacillus laterosporus that can inhibit the growth of drug resistant bacteria

“…laterosporus have been sequenced and and their genomes deposited in Genbank. These include LMG 15441 (under accession number AFRV00000000, [39]), GI-9 (under accession numbers CAGD01000001 to CAGD01000061, [40]), B9 (under accession numbers CP011074-CP011076, [41]), Lak 1210 (under accession number NDIP00000000, [42]), OSY-I1 (under accession number NOLX00000000, [43]), and SA14 (accession number KF718856.1, [https://www.ncbi.nlm. nih.gov/nuccore/KF718856.1]).…”