Fufang-Zhenzhu-Tiaozhi Capsule reduces restenosis via the downregulation of NF-kappaB and inflammatory factors in rabbits

Abstract

BackgroundTo investigate the effects of a Chinese herbal medicine Fufang-Zhenzhu Tiaozhi Capsule (FTZ) on restenosis and elucidate the mechanism of action.MethodsA restenosis model was established by balloon rubbing the endothelium of the abdominal aorta followed by high fat diet. Rabbits were divided into blank control group, restenosis group, FTZ group (0.66 mg/kg/day), atorvastatin group (5 mg/kg/day) and FTZ + atorvastatin group (n = 8). Vascular stenosis was analyzed by X-ray. Serum levels of chemokines and cytokines interleukin-1 (IL-1), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-12 (IL-12), C-reactive protein (CRP), tumor necrosis factor-alpha (TNF-α), monocyte chemoattractant protein-1 (MCP-1), and intercellular adhesion molecule-1 (ICAM-1) were measured by ELISA. The levels of NF-κB, IκB-α, P-IκBα, IKK-α, and P-IKKα/β from injured abdominal arteries were detected by Western blotting.ResultsRestenosis was induced successfully via abdominal artery balloon injuries and high fat diet. Restenosis was significantly decreased in FTZ group compared with restenosis group (P < 0.05). FTZ group had markedly reduced serum lipid levels (P < 0.05). In addition, the levels of TNF-α, IL-1, IL-6, IL-8, IL-12, ICAM-1 and MCP-1 decreased by FTZ treatment (P < 0.05). The expression of NF-κB in the atherosclerotic lesions was significantly attenuated in FTZ group (P < 0.05).ConclusionFTZ could reduce restenosis via reducing NF-κB activity and inflammatory factor expression within the atherosclerotic lesion in a rabbit restenosis model. FTZ may be a new therapeutic agent for restenosis.

A comparison of the effects of monotherapy with rosuvastatin, atorvastatin or ezetimibe versus combination treatment with rosuvastatin-ezetimibe and atorvastatin-ezetimibe on the integrity of vascular endothelial cells damaged by oxidized cholesterol

“…This is in line with Zhang et al . (2018) [ 29 ], who report presenting that administration of atorvastatin (5 mg/kg/day) was associated with reduction of all ICAM-1, IL-1β and TNFα in the serum of rabbits, suggesting it has strong anti-inflammatory potential.…”

Traditional Chinese Medicine Fufang-Zhenzhu-Tiaozhi capsule prevents renal injury in diabetic minipigs with coronary heart disease

“…In a lot of scientific research, TCM has been widely used to treat and control diabetes and its complications such as renal injury [ 40 ]. The FTZ is an innovative prescription with multiple targets to comprehensively prevent and cure glucolipid metabolic disease [ 34 , 36 ]. FTZ effectively inhibited coronary artery incrassation and protected the myocardium against injury in DM-CHD minipigs [ 35 ].…”

Atherosclearosis plaque formation is related to EndMT in diabetic minipigs with coronary heart disease and the effect of Traditional Chinese medicine FTZ Prescription

“…In the cardiovascular aspect, FTZ has the cardioprotective effect in the treatment of DM-CHD and diabetic cardiomyopathy (DCM) through inhibiting ERK and STAT3 signal pathways and activating PI3K/AKT pathway [24,49]. Furthermore, recent studies have shown that FTZ reduced restenosis in the rabbit restenosis model by regulating the adiponectin pathway and reducing NF-κB activity [21,22], but the effect of FTZ on vascular endothelium has not been explicit. In this study, we found that FTZ signi cantly inhibited the EndMT of coronary artery in DM-CHD minipigs and revised the reduced cell viability of HUVECs caused by HG.…”

“…Previous studies have shown that FTZ could inhibit the development of atherosclerosis in the atherosclerosis model and exhibit the cardioprotective effect in the diabetic minipig model with coronary heart disease (DM-CHD) [21][22][23][24]. However, the effect and molecular mechanisms underlying FTZ in DM-CHD are not clear.…”