Fucoidan from Sargassum hemiphyllum inhibits the stemness of cancer stem cells and epithelial-mesenchymal transitions in bladder cancer cells

Sung, Chun-Ju; Wang, Hsiao-Hsien; Sun, Kien-Wen; Hsieh, Chii-Cheng; Huang, Roger D.; Sun, Guang‐Huan; Tang, Shye‐Jye

doi:10.1016/j.ijbiomac.2022.09.047

Cited by 5 publications

(4 citation statements)

References 48 publications

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“…et al reported that fucoidan from S. fusiforme (SFPS-B2) inhibited the growth of the human gastric cancer cell line SGC-7901 with IC 50 of 189.30 μg/mL by the induction of apoptosis via a mitochondrial-mediated pathway [ 19 ]. Fucoidan F2 from the brown alga S. polycystum exhibited a cytotoxic effect against MCF-7 breast cancer cells and HCT-15 colorectal adenocarcinoma cells with IC 50 of 20 and 50 μg/mL, respectively [ 20 ]. On the other hand, sulfated polysaccharide isolated from S. hemiphyllum did not exhibit cytotoxicity against 5637 and T24 bladder cancer cells but suppressed cancer stem cell formation which is implicated in tumor initiation, metastatic spread, drug resistance, and tumor recurrence [ 21 ].…”

Section: Resultsmentioning

confidence: 99%

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Structure and Metabolically Oriented Efficacy of Fucoidan from Brown Alga Sargassum muticum in the Model of Colony Formation of Melanoma and Breast Cancer Cells

Usoltseva,

Zueva,

Malyarenko

et al. 2023

Marine Drugs

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This work reports the detailed structure of fucoidan from Sargassum miticum (2SmF2) and its ability to potentiate the inhibitory effect of glycolysis inhibitor 2-deoxy-d-glucose (2-DG). 2SmF2 was shown to be sulfated and acetylated galactofucan containing a main chain of alternating residues of 1,3- and 1,4-linked α-l-fucopyranose, fucose fragments with monotonous 1,3- and 1,4-type linkages (DP up to 3), α-d-Gal-(1→3)-α-L-Fuc disaccharides, and 1,3,4- and 1,2,4-linked fucose branching points. The sulfate groups were found at positions 2 and 4 of fucose and galactose residues. 2SmF2 (up to 800 µg/mL) and 2-DG (up to 8 mM) were not cytotoxic against MDA-MB-231 and SK-MEL-28 as determined by MTS assay. In the soft agar-based model of cancer cell colony formation, fucoidan exhibited weak inhibitory activity at the concentration of 400 µg/mL. However, in combination with low non-cytotoxic concentrations of 2-DG (0.5 or 2 mM), 2SmF2 could effectively inhibit the colony formation of SK-MEL-28 and MDA-MB-231 cells and decreased the number of colonies by more than 50% compared to control at the concentration of 200 µg/mL. Our findings reveal the metabolically oriented effect of fucoidan in combination with a glycolysis inhibitor that may be beneficial for a therapy for aggressive cancers.

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Section: Resultsmentioning

confidence: 99%

“…These polysaccharides have valuable biological activities and unique biomedical applications. In particular, it is known that several fucoidans isolated from algae of the genus Sargassum have an anticancer effect in various cell lines [ 16 , 17 , 18 , 19 , 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Structure and Metabolically Oriented Efficacy of Fucoidan from Brown Alga Sargassum muticum in the Model of Colony Formation of Melanoma and Breast Cancer Cells

Usoltseva,

Zueva,

Malyarenko

et al. 2023

Marine Drugs

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“…Since tumour migration and invasion ability are closely related to EMT, we want to explore whether fucoidan can inhibit the progression of hemangioma by inhibiting the EMT process. It has been reported that fucoidan can inhibit the proliferation of tumour stem cells in bladder cancer 40 and prevent EMT by blocking extracellular matrix degradation in endothelial cells, thus hindering tumour metastasis. 41 In our study, data showed that fucoidan exhibited an inhibitory effect on the proliferation of EOMA cells and promoted apoptosis, especially in high concentrations.…”

Section: Discussionmentioning

confidence: 99%

Fucoidan suppresses proliferation and epithelial‐mesenchymal transition process via Wnt/β‐catenin signalling in hemangioma

Zhu,

Luo,

et al. 2024

Experimental Dermatology

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Hemangioma is a common benign tumour that usually occurs on the skin of the head and neck, particularly among infants. The current clinical treatment against hemangioma is surgery excision, however, application of drug is a safer and more economical therapy for children suffering from hemangioma. As a natural sulfated polysaccharide rich in brown algae, fucoidan is widely recognized for anti‐tumour bioactivity and dosage safety in humans. This study aims to demonstrate the anti‐tumour effect and underlying mechanism of fucoidan against hemangioma in vivo and in vitro. We investigated the effects of fucoidan by culturing hemangioma cells in vitro and treating BALB/c mice bearing with hemangioma. At first, we measured the cell proliferation and migration ability through in vitro experiments. Then, we tested the expression of epithelial‐mesenchymal transition (EMT) and Wnt/β‐catenin pathway‐related biomarkers by western blot and qPCR. Furthermore, we applied β‐catenin‐specific inhibitor, XAV939, to determine whether fucoidan suppressed EMT via the Wnt/β‐catenin pathway in hemangioma cells. In vivo experiments, we applied oral gavage of fucoidan to treat EOMA‐bearing mice, along with evaluating the safety and efficacy of fucoidan. We found that fucoidan remarkably inhibits the proliferation and EMT ability of hemangioma cells, which is dependent on the Wnt/β‐catenin pathway. These results suggest that fucoidan exhibits tumour inhibitory effect on aggressive hemangioma via regulating the Wnt/β‐catenin signalling pathway both in vitro and in vivo, providing a new potent drug candidate for treating hemangioma.

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“…Sung et al . [ 19 ] reported that fucoidan treatment inhibited bladder cancer cell migration and suppressed stress fibers aggregation including filopodial protrusion in bladder cancer T24 and 5637 cells. In addition, fucoidan also inhibited cancer cell migration in many cancer cell types including HepG2-SR, CRPCa, and mCRPCa cells [ 20 21 ].…”

Section: Discussionmentioning

confidence: 99%

Antimetastatic effect of fucoidan against non-small cell lung cancer by suppressing non-receptor tyrosine kinase and extracellular signal-related kinase pathway

Muneerungsee,

Tanasawet,

Sukketsiri

2023

Nutr Res Pract

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BACKGROUND/OBJECTIVES Fucoidan, a polysaccharide content in brown algae, has been reported to inhibit the growth of cancer cells. The present study aimed to investigate the suppression effects of fucoidan on A549 non-small cell lung cancer cells migration. MATERIALS/METHODS The anti-migratory activity of fucoidan in A549 cells was examined by wound healing assay and phalloidin-rhodamine staining in response to fucoidan (0–100 µg/mL) treatment for 48 h. Western blot analysis was performed to clarify the protein expressions relevant to migratory activity. RESULTS Fucoidan (25–100 µg/mL) significantly suppressed A549 cells migration together with reduced the intensity of phalloidin-rhodamine which detect filopodia and lamellipodia protrusions at 48 h of treatment. The protein expression indicated that fucoidan significantly suppressed the phosphorylation of focal adhesion kinase (FAK), Src, and extracellular signal-related kinase (ERK). In addition, the phosphorylation of p38 in A549 cells was found to be increased. CONCLUSIONS Our data conclude that fucoidan exhibits anti-migratory activities against lung cancer A549 cells mediated by inhibiting ERK1/2 and FAK-Src pathway.

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Fucoidan from Sargassum hemiphyllum inhibits the stemness of cancer stem cells and epithelial-mesenchymal transitions in bladder cancer cells

Cited by 5 publications

References 48 publications

Structure and Metabolically Oriented Efficacy of Fucoidan from Brown Alga Sargassum muticum in the Model of Colony Formation of Melanoma and Breast Cancer Cells

Structure and Metabolically Oriented Efficacy of Fucoidan from Brown Alga Sargassum muticum in the Model of Colony Formation of Melanoma and Breast Cancer Cells

Fucoidan suppresses proliferation and epithelial‐mesenchymal transition process via Wnt/β‐catenin signalling in hemangioma

Antimetastatic effect of fucoidan against non-small cell lung cancer by suppressing non-receptor tyrosine kinase and extracellular signal-related kinase pathway

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