Frozen Human Epidermal Allogeneic Cultures Promote Rapid Healing of Facial Dermabrasion Wounds

Abstract

Epidermal allogenic cultures, preserved by freezing, promoted significantly faster reepithelization and reduced pain intensity of dermabraded facial wounds, suggesting that they could be used routinely to improve the recovery from dermabrasion.

Factors that Influence Healing in Chronic Venous Ulcers Treated with Cryopreserved Human Epidermal Cultures

“…CEC have been successfully used in the treatment of burns 33,37,38 , leg ulcers 39–41 , split‐thickness skin graft donor sites 42,43 , epidermolysis bullosa 44 , surgical wounds post mohs 45 , decubitus ulcers, amputation stump ulcers, rheumatoid ulcers, scleroderma, split‐thickness skin graft donor sites, laser removal sites for tattoos 46,47 , and facial dermabrasion wounds. 48 These epidermal cultures seem to act as a potent stimulus to wound healing, but do not survive permanently on the wound bed. 33,34 Their postulated mechanism of action is through release of multiple cytokines that stimulate epithelialization from the wound periphery as well as from remnant epidermal appendages.…”

Involvement of Keratinocyte Activation Phase in Cutaneous Graft Healing: Comparison of Full-Thickness and Split-Thickness Skin Grafts

“…30,31 The enhanced keratinocyte activity in PESG may play an important role in graft take through enhanced ␤ 1 integrin expression, which is associated with cell-matrix adhesion, similar to cultured keratinocyte sheet graft take. [13][14][15] SW covered by FTSG contracted more than SW covered by STSG at 8 weeks postgrafting. B) There were no significant differences on DW between PESG, STSG, and FTSG.…”

“… 30,31 The enhanced keratinocyte activity in PESG may play an important role in graft take through enhanced β 1 integrin expression, which is associated with cell‐matrix adhesion, similar to cultured keratinocyte sheet graft take. 13–15 …”

Scar Revision Via Resurfacing

“…An alternate option is frozen human epidermal keratinocytes as a biologic dressing, felt by some to promote rapid reepithelialization. 11 Soap and water are used to clean the area gently, and the patient is instructed to avoid hydrogen peroxide or astringents on the area. A warm water soaked cloth can be applied to the face to soften crusts.…”