From large to small: the immunohistochemical panel in the diagnosis of early hepatocellular carcinoma

Vasuri, Francesco; Malvi, Deborah; Bonora, Sonia; Fittipaldi, Silvia; Renzulli, Matteo; Tovoli, Francesco; Golfieri, Rita; Bolondi, Luigi; D’Errico, Antonietta

doi:10.1111/his.13389

Cited by 13 publications

(4 citation statements)

References 22 publications

(55 reference statements)

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“…In certain case, the chaperone can be both structurally and functionally normal but just get participated in pathways that lead to disease. For instance, the post-translational modification of the chaperone could alter its subcellular localization and subsequent function 17–20,28–30. Moreover, high expression of chaperone can lead to superabundant protein synthesis, which is a common feature of a wide variety of tumors.…”

Section: Discussionmentioning

confidence: 99%

“…HSP10 and HSP70 proteins were located in the cytoplasm. A semi-quantitative evaluation of HSP10 and HSP70 expression was performed using a method described in the literature26–28: staining intensity for HSP10 and HSP70 were divided into four grades (intensity scores): as 0 (negative, no staining), 1 (weak, light brown), 2 (moderate, brown), and 3 (strong, dark brown). The positive percentage was divided into five grades (percentage scores): 0 (0%), 1 (1–25%), 2 (26–50%), 3 (51–75%), and 4 (76–100%).…”

Section: Methodsmentioning

confidence: 99%

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<p>Increased expression of heat shock protein (HSP) 10 and HSP70 correlates with poor prognosis of nasopharyngeal carcinoma</p>

Feng

Zhan

Zhang

et al. 2019

CMAR

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BackgroundHeat shock proteins (HSPs) are a large family of chaperones implicating in occurrence and progression of tumor. In our previous study, we found HSP10 correlates with poor prognosis of oral squamous cell carcinoma and astrocytoma. HSP70 is also an important part of this family and whether the alterations of HSP10 and HSP70 expression and their common expression correlates with carcinogenesis and progression of nasopharyngeal carcinoma (NPC) has not been reported.MethodIn this study, we investigate the correlation between the expression of HSP10 and HSP70 and clinicopathological characteristics in NPC by immunohistochemistry (IHC).ResultsResults indicated that positive expression of HSP10 and HSP70 was higher in NPC tissues (both P<0.001). Positive expression of HSP10 and HSP70 proteins, and common positive expression of the two HSPs analyzed in advanced clinical stages were higher than that in early clinical stages (All P<0.05). There was significantly higher expression of HSP10, HSP70, and common expression in NPC with LNM (lymph node metastasis) compared with NPC without LNM (All P<0.05). Interestingly, positive expression of HSP10 and HSP70 proteins and common expression had an evidently inverse correlation with survival status (All P<0.05). Spearman’s correlation analysis showed expression of HSP10 was positively associated with HSP70 (r=0.407, P<0 0.001). Kaplan–Meier analysis showed that the overall survival rates for NPC patients with positive expression of HSP10 and HSP70 and common expression were significantly lower than these patients with negative expression (All P<0.05). Furthermore, positive expression of HSP10 and HSP70 proteins was identified as independent poor prognostic factors for NPC patients (both P<0.05) by Cox regression analysis.Conclusion In conclusion, HSP10 and HSP70 can serve as the poor prognostic factors for NPC patients.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

<p>Increased expression of heat shock protein (HSP) 10 and HSP70 correlates with poor prognosis of nasopharyngeal carcinoma</p>

Feng

Zhan

Zhang

et al. 2019

CMAR

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“…Other pathologic variables have also been analyzed and correlated to tumor behaviors, such as encapsulation, intratumor steatosis, and tumor-infiltrating cells [ 5 , 6 , 7 ]. The tumor heterogeneity in HCC indicates that pathological subtypes could be helpful in the subclassification of HCC in addition to tumor staging, where glutamine synthase (GS), glypican-3 (GPC-3), heat shock protein 70 (Hsp70), and enhancer of zeste homologue 2 (EZH2) are the common markers for HCC diagnosis [ 8 ]. Biliary/stem cell markers and Wnt/β-catenin signaling have been used for the three-group classification of HCC [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

The Vessels That Encapsulate Tumor Clusters (VETC) Pattern Is a Poor Prognosis Factor in Patients with Hepatocellular Carcinoma: An Analysis of Microvessel Density

Huang

Lin

Huang

et al. 2022

Cancers

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The outcomes of patients with hepatocellular carcinoma (HCC) are unsatisfactory because of its high recurrence rate. The Vessels that encapsulate tumor clusters (VETC) pattern is a unique vascular structure. In this study, we investigated the clinical–pathological features of HCC patients with the VETC pattern. We retrospectively reviewed patients with HCC who underwent curative hepatectomy at Chang Gung Memorial Hospital between 2007 and 2013. The form of the VETC pattern was established using an anti-CD31 stain. The results were classified into positive (VETC+) and negative (VETC−) patterns. We investigated and compared demographic data between these two groups. Overall, 174 patients were classified into either the VETC+ or VETC− groups. The median followed-up period was 80.5 months. There were significant differences in the number of hepatitis B carriers, the occurrence of vascular invasion, tumor size, TNM staging, microvessel density, and recurrence (all p < 0.05). Regarding the prediction of disease-free survival, after COX regression multivariate analysis, VETC+ remained independently associated with recurrent episodes (p = 0.003). The intra-tumoral microvessel density, demonstrated by CD-31, was the only clinical–pathological feature independently associated with VETC+. Our study demonstrated that the VETC pattern is an independent factor of poor prognosis for DFS. Higher intra-tumoral microvessel density was significantly associated with the VETC pattern. Further studies are needed to validate our findings.

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“…In cases where non-invasive imaging results are ambiguous, HCC is diagnosed by histologic features of biopsy tissue. Accuracy of diagnosis is improved by immunohistochemical (IHC) analysis for established biomarkers, such as glypican 3 (GPC3), heat shock protein 70 (HSP70), and glutamine synthetase (GS) [ 18 – 20 ]. However, the molecular complexity of HCC presents challenges finding reliable biomarkers for all patients [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Using quantitative immunohistochemistry in patients at high risk for hepatocellular cancer

et al. 2022

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Hepatocellular carcinoma (HCC) is the primary form of liver cancer and a major cause of cancer death worldwide. Early detection is key to effective treatment. Yet, early diagnosis is challenging, especially in patients with cirrhosis, who are at high risk of developing HCC. Dysfunction or loss of function of the transforming growth factor β (TGF-β) pathway is associated with HCC. Here, using quantitative immunohistochemistry analysis of samples from a multi-institutional repository, we evaluated if differences in TGF-β receptor abundance were present in tissue from patients with only cirrhosis compared with those with HCC in the context of cirrhosis. We determined that TGFBR2, not TGFBR1, was significantly reduced in HCC tissue compared with cirrhotic tissue. We developed an artificial intelligence (AI)-based process that correctly identified cirrhotic and HCC tissue and confirmed the significant reduction in TGFBR2 in HCC tissue compared with cirrhotic tissue. Thus, we propose that a reduction in TGFBR2 abundance represents a useful biomarker for detecting HCC in the context of cirrhosis and that incorporating this biomarker into an AI-based automated imaging pipeline could reduce variability in diagnosing HCC from biopsy tissue.

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From large to small: the immunohistochemical panel in the diagnosis of early hepatocellular carcinoma

Cited by 13 publications

References 22 publications

<p>Increased expression of heat shock protein (HSP) 10 and HSP70 correlates with poor prognosis of nasopharyngeal carcinoma</p>

<p>Increased expression of heat shock protein (HSP) 10 and HSP70 correlates with poor prognosis of nasopharyngeal carcinoma</p>

The Vessels That Encapsulate Tumor Clusters (VETC) Pattern Is a Poor Prognosis Factor in Patients with Hepatocellular Carcinoma: An Analysis of Microvessel Density

Using quantitative immunohistochemistry in patients at high risk for hepatocellular cancer

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