From Bio-waste to Bone Substitute

Ressler, Antonia; Gudelj, Ana; Zadro, Karla; Antunović, Maja; Cvetnić, Matija; Ivanković, Marica; Ivanković, Hrvoje

doi:10.15255/cabeq.2020.1783

Cited by 22 publications

(19 citation statements)

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“…Utku et al successfully prepared fibroin/hydroxyapatite scaffolds by dissolving fibroin and hydroxyapatite in phosphate buffer [2]. Ressler et al learned from research that the nanocomposite structure of bone can be simulated by chitosan/hydroxyapatite (CS/HAp) composite scaffold [3]. Vurat et al developed a novel osteogenic composite nanocoating for titanium surfaces.…”

Section: Related Workmentioning

confidence: 99%

“…It acts as a temporary extracellular matrix, providing a favorable microenvironment for growth, thereby promoting cell differentiation. (3) Finally, it simulates the parallel arrangement of tendon collagen fibers to prepare parallel ordered nano-parallel material composite proteins. is can induce the directional differentiation of fibroblasts to the tendon system, so as to achieve the effect of repairing the nerve tendon.…”

Section: Introductionmentioning

confidence: 99%

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Postcare for Repairing Nerve and Tendon Injury Based on Biomimetic Nano-Parallel Material Composite Protein

Tan

Tang

2022

Advances in Materials Science and Engineering

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Natural tendons are composed of ordered parallel arrangement of bundles of type I collagen fibers. It is responsible for transmitting the forces generated by the bones and muscles to move the body. Tendon injuries are common sports injuries, accounting for 50% of sports injuries. In adulthood, factors such as few tendon tissue cells and poor blood supply make it difficult to heal on their own after injury. Therefore, restoring the structure and function of native tendon tissue is still an unsolved problem. The purpose of this study is to study the use of biomimetic nano-parallel material composite proteins to induce the directional differentiation of stem cells to tendon lineages to promote muscle regeneration and repair. This study proposes to prepare composite protein nanomaterials by simulating the parallel arrangement of tendon collagen fibers. This can facilitate the directed differentiation of fibroblasts into tendon lineages. It can use the BP algorithm in the neural network algorithm to simulate the parallel arrangement of tendon collagen fibers, which is more efficient than other schemes. The experimental results in this study show that the rat cells are repaired after about 6 weeks. Tendon repair enters a remodeling phase with reduced cell numbers, collagen, and mucopolysaccharide synthesis. During this period, tendon repair gradually changes from the cellular level to the tissue level, and tenocyte metabolism remains high. Tenocytes and collagen fibers are aligned in the direction of stress. A higher proportion of collagen 1 synthesis occurs during this period. Collagen 1 accounts for 65% to 80% of the dry weight of the tendon, which plays the most important role in the mechanical properties of the tendon.

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Section: Related Workmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Postcare for Repairing Nerve and Tendon Injury Based on Biomimetic Nano-Parallel Material Composite Protein

Tan

Tang

2022

Advances in Materials Science and Engineering

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“…Scaffolds porosity was measured following the Archimedes method as previously described by Ressler et al [22], by immersing each scaffold in ethanol (ρ = 0.789 g cm -3 ) at room temperature. The porosity (%) was then calculated from the fraction of the volume of the pores (V pore ) within the total volume of the scaffold (V CS/HA ) based on Eq.…”

Section: Characterization Methodsmentioning

confidence: 99%

“…However, the trace elements detected in the phosphate ore used in this work can play a key role to obtain a multi-substituted HA that mimic the inorganic phase of human bone. They can improve its biological performance as they can increase proliferation and differentiation of osteoblast cells, and decrease osteoclast cells activity [22]. [31].…”

Section: Characterization Of Np Orementioning

confidence: 99%

Preparation and Characterization of Biocomposite Based on Chitosan and Biomimetic Hydroxyapatite Derived from Natural Phosphate Rocks

Brahimi

Ressler

Boumchedda

et al. 2021

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Highly-porous composite structures based on chitosan (CS) and hydroxyapatite (HA) have been prepared using the freeze-gelation method. Biomimetic HA powder was first synthesized by a wet precipitation method using natural phosphate (NP) ore as a source of calcium and phosphorus ions. The powders and scaffolds have been characterized by elemental analysis, Fourier transform infrared spectrometry, X-ray diffraction, Rietveld refinement studies, scanning electron microscopy and cell viability test. The raw precipitated powder was composed of HA and amorphous calcium phosphate (ACP). In the obtained powder Mg2+, SiO42−, Sr2+, Na+, and Fe2+ ions were detected as a result of using NP mineral as precursor. After heat treatment over 750°C, β-tricalcium phosphate Mg substituted (β-TCMP) was detected. The culture of human embryonic kidney cells indicated non cytotoxicity of the as-prepared powder. CS/HA scaffolds with different weight ratios (100/0; 90/10; 80/20; 70/30) have been prepared. The CS/HA scaffolds have shown highly porous structure with well interconnected pores and homogeneously dispersed HA particles. Swelling capacity measured in physiological conditions has shown that the addition of HA into the CS matrix efficiently decreased the swelling percentage of the CS/HA scaffolds. The results obtained suggest that composite scaffolds prepared using CS and biomimetic HA derived from an abundant raw material may have effective potential as biocompatible materials for bone tissue engineering.

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“…HA is a compound that has a hexagonal structure with white solids [19]. Because it has biocompatibility, bioactivity [20][21][22] and significant osteoinductivity [23], hydroxyapatite has been widely used as a bone substitute to fill bone defects, such as matrix scaffolding for tissue engineering [24] and as a coating on biomedical implants [20]. In biomedical applications, biomaterials must have reliable biocompatibility capabilities [25].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Degradation and Cytotoxicity of Eggshell-Based Hydroxyapatite: A Systematic Review and Meta-Analysis

et al. 2021

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Objective: This review focuses on the in vitro degradation of eggshell-based hydroxyapatite for analyzing the weight loss of hydroxyapatite when applied in the human body. Cytotoxicity tests were used to observe cell growth and morphological effects. A systematic review and meta-analysis were conducted to observe the weight loss and viable cells of hydroxyapatite when used for implants. Method: Based on the Population, Intervention, Comparison, and Outcome (PICO) strategy, the articles used for literature review were published in English on SCOPUS, PubMed, and Google Scholar from 1 January 2012 to 22 May 2021. Data regarding existing experiments in the literature articles the in vitro degradation and cytotoxicity testing of eggshell-based hydroxyapatite determined the biocompatibility of the materials. A meta-analysis was conducted to calculate the mean difference between the solutions and soaking times used for degradation and the stem cells used for cytotoxicity. Results: From 231 relevant studies, 71 were chosen for full-text analysis, out of which 33 articles met the inclusion criteria for degradation and cytotoxicity analysis. A manual search of the field of study resulted in three additional articles. Thus, 36 articles were included in this systematic review. Significance: The aim of this study was to highlight the importance of the biocompatibility of eggshell-based hydroxyapatite. The weight loss and viability cells of eggshell-based hydroxyapatite showed optimum results for viable cells requirements above 70%, and there is a weight loss of eggshell-based hydroxyapatite for a material implant. The meta-analysis indicated significant differences in the weight loss of eggshell-based hydroxyapatite materials with different soaking times and solutions used. The various kinds of stem cells for incubation of cultured cells in contact with a device, either directly or through diffusions with various kinds of stem cells from animals and humans, yielded viability cells above 70%.

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From Bio-waste to Bone Substitute

Cited by 22 publications

References 0 publications

Postcare for Repairing Nerve and Tendon Injury Based on Biomimetic Nano-Parallel Material Composite Protein

Postcare for Repairing Nerve and Tendon Injury Based on Biomimetic Nano-Parallel Material Composite Protein

Preparation and Characterization of Biocomposite Based on Chitosan and Biomimetic Hydroxyapatite Derived from Natural Phosphate Rocks

In Vitro Degradation and Cytotoxicity of Eggshell-Based Hydroxyapatite: A Systematic Review and Meta-Analysis

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