From Basic Research to Clinical Application of Placental Proteins—A Workshop Report

Than, Nándor Gábor; Bohn, H.; Tóth, Péter T.; Bersinger, NA; Grudzinskas, J. G.; Bischof, P.

doi:10.1016/j.placenta.2004.01.021

Cited by 7 publications

(8 citation statements)

References 10 publications

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“…Burger et al reported that serum PP13 levels in patients with preeclampsia during the second and third trimester were significantly higher than in normal serum. [9][10][11] However, a previous study revealed that mRNA expression of PP13 was decreased in the cellular components of blood from pregnant women with preeclampsia, in comparison to normal blood (data not shown). The present results proved the cell-level expression of PP13 in the syncytiotrophoblasts from preeclamptic patients to be lower than those from normal pregnancies.…”

Section: Discussionmentioning

confidence: 82%

“…Although the function of PP13 is not clear, it is reported that PP13 is associated with implantation and maternal artery remodeling, and that PP13 prevents erythrocyte adhesion in the area with reduced blood flow such as intervillous space. [9][10][11] Furthermore, PP13 is suggested to have special immune functions at the feto-maternal interface. [9][10][11] Placental protein 13 is known to be placenta-specific.…”

Section: Discussionmentioning

confidence: 99%

“…Placental protein 13 is a 32-kd dimer protein and is thought to be involved in implantation and maternal artery remodeling. [9][10][11] In the present study, to assess the role of PP13 in the pathogenesis of preeclampsia, the expression of PP13 messenger RNA (mRNA) was investigated in the syncytiotrophoblasts and extravillous trophoblasts (EVTs) from the placenta of preeclamptic women. Furthermore, the expression of PP13 mRNA was investigated in first trimester trophoblasts from residual samples of chorionic villus sampling (CVS) obtained for fetal karyotyping, who subsequently developed preeclampsia.…”

Section: Introductionmentioning

confidence: 99%

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PP13 mRNA Expression in Trophoblasts From Preeclamptic Placentas

et al. 2009

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To assess the role of placental protein 13 (PP13) in the pathogenesis of preeclampsia, the messenger RNA expression of PP13 was investigated in the trophoblasts from first and third trimester placenta. First, syncytiotrophoblasts and extravillous trophoblasts were separated from frozen section of third trimester placenta obtained from pregnant women with and without preeclampsia by laser microdissection. The PP13 levels of syncytiotrophoblasts were significantly higher than those of extravillous trophoblasts. The expression levels in syncytiotrophoblasts from the preeclampsia were significantly lower than those from normal. Next, the PP13 expression was assessed in trophoblasts from residual samples of chorionic villus sampling at 11 weeks of gestation, who subsequently developed preeclampsia. The levels in the preeclampsia group were significantly lower than those of normal cases. These findings indicate that an alteration in the PP13-messenger RNA expression in the trophoblasts may be associated with the pathogenesis of preeclampsia.

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Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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PP13 mRNA Expression in Trophoblasts From Preeclamptic Placentas

et al. 2009

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“…Expression of the unique placental proteins in different stages maintains the pregnancy and supports the proper development of the fetus. 3 The most important cells of a placenta are trophoblast cells. Without trophoblast cells, a pregnancy could not proceed.…”

Section: Introductionmentioning

confidence: 99%

Proteome Differences in the First- and Third-Trimester Human Placentas

2015

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Placenta is a transient and unique pregnancy tissue that supports the fetus nutritionally and metabolically. Expression of the unique placental proteins in different stages may influence the development of the fetus as well as the pregnancy outcome. The present study aimed to compare the total placental proteome differences between the normal first-and third-trimester human placentas. In the current study, placental proteome was compared between normal first-and third-trimester placentas using 2-dimensional polyacrylamide gel electrophoresis method for separation and matrix-assisted laser desorption/ionization time-of flight mass spectrometry technique for identification of the proteins. Despite the overall similarities, comparison of the mean intensity of the protein spots between the first-and third-trimester placental proteomes revealed that 22 spots were differentially expressed (P < .05) among which 11 distinct spots were successfully identified. Of the 11 differentially expressed proteins, 4 were increased (protein disulfide isomerase, tropomyosin 4 isoform 2, enolase 1, and 78-kDa glucose-regulated protein), while the remaining 7 (actin g1 propeptide, heat shock protein gp96, a1-antitrypsin, EF-hand domain family member D1, tubulin a1, glutathione S-transferase, and vitamin D binding protein) showed decreased expression in the placentas from the first-trimester compared to the full-term ones. In summary, the results of the present study as the first research on the comparison of the first-and thirdtrimester human placental proteomes introduced a group of 11 proteins with altered expression. Interestingly, some of these proteins are reported to be altered in pregnancy-related disorders.

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“…There was a significant increase in the level of annexin II, a protein associated with proliferation (Menaa et al 1999) and fibrinolysis, under hypoxic conditions (Rao et al 1994). Functional proteomic studies have identified annexin II as a protein that binds to placental protein 13 (PP13)/galectin 13, whose dimerization may have a role in affecting oxygen changes in the placenta (Than et al 2004).…”

Section: Proteomic Studies On Early Pregnancymentioning

confidence: 99%

An emerging role for comprehensive proteome analysis in human pregnancy research

Shankar¹,

Gude²,

Cullinane³

et al. 2005

Reproduction

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Elucidation of underlying cellular and molecular mechanisms is pivotal to the comprehension of biological systems. The successful progression of processes such as pregnancy and parturition depends on the complex interactions between numerous biological molecules especially within the uterine microenvironment. The tissue-and stage-specific expression of these biomolecules is intricately linked to and modulated by several endogenous and exogenous factors. Malfunctions may manifest as pregnancy disorders such as preterm labour, pre-eclampsia and fetal growth restriction that are major contributors to maternal and perinatal morbidity and mortality. Despite the immense amount of information available, our understanding of several aspects of these physiological processes remains incomplete. This translates into significant difficulties in the timely diagnosis and effective treatment of pregnancy-related complications. However, the emergence of powerful mass spectrometry-based proteomic techniques capable of identifying and characterizing multiple proteins simultaneously has added a new dimension to the field of biomedical research. Application of these high throughput methodologies with more conventional techniques in pregnancy-related research has begun to provide a novel perspective on the biochemical blueprint of pregnancy and its related disorders. Further, by enabling the identification of proteins specific to a disease process, proteomics is likely to contribute, not only to the comprehension of the underlying pathophysiologies, but also to the clinical diagnosis of multifactorial pregnancy disorders. Although the application of this technology to pregnancy research is in its infancy, characterization of the cellular proteome, unearthing of functional networks and the identification of disease biomarkers can be expected to significantly improve maternal healthcare in the future.

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From Basic Research to Clinical Application of Placental Proteins—A Workshop Report

Cited by 7 publications

References 10 publications

PP13 mRNA Expression in Trophoblasts From Preeclamptic Placentas

PP13 mRNA Expression in Trophoblasts From Preeclamptic Placentas

Proteome Differences in the First- and Third-Trimester Human Placentas

An emerging role for comprehensive proteome analysis in human pregnancy research

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