FRET-reporter nanoparticles to monitor redox-induced intracellular delivery of active compounds

Abstract

FRET-reporter particles for redox-induced release of active compounds in cells were developed. This particle system allowed following the intracellular cleavage of delivered compounds after particle internalization.

The local field dependent effect of the critical distance of energy transfer between nanoparticles

“…Note that, in the case of NSET with a gold nanoparticle acceptor, the R À 4 distance dependence is observed for large particles [2,20]. For the case of standard FRET with two molecular dyes, we take the vertex parts as dipole moment of atoms A μ → , D μ → , the standard , where a is the radius, P λ is the plasmon wavelength, and g is defined as…”

“…FRET can be used as spectroscopic ruler in various areas such as the interaction of biological molecules in vitro and in vivo assays in cellular research, nucleic acid analysis, signal transduction, light harvesting and metallic nanomaterial etc. Based on the mechanism of FRET a variety of novel chemical sensors and biosensors have been developed [1][2][3][4][5]. Conventional FRET exhibits the R À 6 dependence law of energy transfer rate and characterized by the Förster distance or critical distance R 0 , which defined as the distance at which the energy transfer efficiency is 50%.…”

Core-Shell Structures of Upconversion Nanocrystals Coated with Silica for Near Infrared Light Enabled Optical Imaging of Cancer Cells

“…UCL emission of core-shell nanostructures was mainly localized in the cytoplasm of the cells, and we did not observe luminescence in the nucleus. The overlay image is presented in Figure 6 d. The UCNC@SiO 2 core-shell nanostructures showed sufficiently good cell viability and efficacy of cellular labeling for optical imaging applications even without further surface functionalization, which could be readily applied on to the silica coating for promoting further cellular uptake or other functions [ 64 ]. Importantly, auto-fluorescence of MDA-MB-231 cells can be removed by excitation at longer wavelengths, i.e., 980 nm laser.…”

New Drug‐Structure‐Directing Agent Concept: Inherent Pharmacological Activity Combined with Templating Solid and Hollow‐Shell Mesostructured Silica Nanoparticles

“…Synthesis of Fluorescein Isothiocyanate ( FITC) Doped MSN Nanoparticles : 1 mg FITC was added to a solution of 100 mg of MSN nanoparticles in 20 mL of methanol, and the stirring was continued for 8 h at room temperature. The final orange MSN nanoparticles were repeatedly washed with methanol and water and were subsequently desiccated under vacuum to remove the solvents …”