Frequency of resistance to Vip3Aa20 toxin from Bacillus thuringiensis in Spodoptera frugiperda (Lepidoptera: Noctuidae) populations in Brazil

Bernardi, Oderlei; Bernardi, Daniel; Ribeiro, Rebeca Sadigursky; Okuma, Daniela M.; Salmeron, Eloisa; Fatoretto, Júlio; Medeiros, Fernanda Carvalho Lopes; Burd, Tony; Omoto, Celso

doi:10.1016/j.cropro.2015.06.006

Cited by 61 publications

(64 citation statements)

References 39 publications

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“…Further studies on the resistant H. punctigera strain confirmed that there was no linkage between the Vip3A and the Cry2Ab resistance loci (104). A study on the presence of Vip3Aa resistance alleles in field populations of S. frugiperda from different states of Brazil, using the F 2 method, estimated an overall frequency of 0.0009, which is relatively low (105).…”

Section: Resistance and Cross-resistancementioning

confidence: 99%

Bacterial Vegetative Insecticidal Proteins (Vip) from Entomopathogenic Bacteria

Chakroun

Banyuls

Bel

et al. 2016

Microbiol Mol Biol Rev

238

246

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SUMMARYEntomopathogenic bacteria produce insecticidal proteins that accumulate in inclusion bodies or parasporal crystals (such as the Cry and Cyt proteins) as well as insecticidal proteins that are secreted into the culture medium. Among the latter are the Vip proteins, which are divided into four families according to their amino acid identity. The Vip1 and Vip2 proteins act as binary toxins and are toxic to some members of the Coleoptera and Hemiptera. The Vip1 component is thought to bind to receptors in the membrane of the insect midgut, and the Vip2 component enters the cell, where it displays its ADP-ribosyltransferase activity against actin, preventing microfilament formation. Vip3 has no sequence similarity to Vip1 or Vip2 and is toxic to a wide variety of members of the Lepidoptera. Its mode of action has been shown to resemble that of the Cry proteins in terms of proteolytic activation, binding to the midgut epithelial membrane, and pore formation, although Vip3A proteins do not share binding sites with Cry proteins. The latter property makes them good candidates to be combined with Cry proteins in transgenic plants (Bacillus thuringiensis-treated crops [Bt crops]) to prevent or delay insect resistance and to broaden the insecticidal spectrum. There are commercially grown varieties of Bt cotton and Bt maize that express the Vip3Aa protein in combination with Cry proteins. For the most recently reported Vip4 family, no target insects have been found yet.

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Section: Resistance and Cross-resistancementioning

confidence: 99%

Bacterial Vegetative Insecticidal Proteins (Vip) from Entomopathogenic Bacteria

Chakroun

Banyuls

Bel

et al. 2016

Microbiol Mol Biol Rev

238

246

View full text Add to dashboard Cite

show abstract

“…To our knowledge, resistance to Vip3Aa had only been obtained in Spodoptera litura (23), Spodoptera frugiperda (24), Helicoverpa armigera, and Helicoverpa punctigera (22). Our results show that the development of Vip3Aa resistance in the H. virescens population was rapid, reaching a level of 200-fold resistance after 9 selection episodes and over 2,000-fold resistance after 13 episodes of selection.…”

Section: Discussionmentioning

confidence: 73%

“…Very few studies have been carried out so far to select for resistance to Vip3 proteins, and the biochemical bases of resistance to these proteins are still unknown (22)(23)(24). In this article, we describe the result of the successful selection for Vip3Aa resistance of a field-derived population of H. virescens and the subsequent work carried out to characterize this population in terms of the genetic bases of resistance and whether Vip3Aa resistance confers cross-resistance to other B. thuringiensis insecticidal proteins.…”

mentioning

confidence: 99%

Bacillus thuringiensis Vip3Aa Toxin Resistance in Heliothis virescens (Lepidoptera: Noctuidae)

Pickett

Gulzar

Ferré

et al. 2017

Appl Environ Microbiol

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Laboratory selection with Vip3Aa of a field-derived population of Heliothis virescens produced Ͼ2,040-fold resistance in 12 generations of selection. The Vip3Aa-selected (Vip-Sel)-resistant population showed little cross-resistance to Cry1Ab and no cross-resistance to Cry1Ac. Resistance was unstable after 15 generations without exposure to the toxin. F 1 reciprocal crosses between Vip3Aa-unselected (Vip-Unsel) and Vip-Sel insects indicated a strong paternal influence on the inheritance of resistance. Resistance ranged from almost completely recessive (mean degree of dominance [h] ϭ 0.04 if the resistant parent was female) to incompletely dominant (mean h ϭ 0.53 if the resistant parent was male). Results from bioassays on the offspring from backcrosses of the F 1 progeny with Vip-Sel insects indicated that resistance was due to more than one locus. The results described in this article provide useful information for the insecticide resistance management strategies designed to overcome the evolution of resistance to Vip3Aa in insect pests.IMPORTANCE Heliothis virescens is an important pest that has the ability to feed on many plant species. The extensive use of Bacillus thuringiensis (Bt) crops or spray has already led to the evolution of insect resistance in the field for some species of Lepidoptera and Coleoptera. The development of resistance in insect pests is the main threat to Bt crops. The effective resistance management strategies are very important to prolong the life of Bt plants. Lab selection is the key step to test the assumption and predictions of management strategies prior to field evaluation. Resistant insects offer useful information to determine the inheritance of resistance and the frequency of resistance alleles and to study the mechanism of resistance to insecticides.

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“…Maize hybrids expressing Vip3Aa20 protein (MIR162 and Bt11×MIR162×GA21) have been commercialized in Brazil since (CTNBIO, 2014. However, the cultivated area with these Bt maize events is small, less than 10% of the total maize area (BERNARDI et al, 2015). Many factors may contribute to variation in baseline susceptibility reported in Brazil and in different laboratories around the world.…”

Section: Discussionmentioning

confidence: 99%

“…Hence, there is no cross-resistance between these insecticidal proteins in insects (JACKSON et al, 2007;SENA;HERNANDEZ-RODRIGUEZ;FERRÃO, 2009;KURTZ, 2010;GULZAR et al, 2012). In Brazil, the adoption of Vip3Aa20 maize is still low (BERNARDI et al, 2015). However, its cultivation will tend to increase in next few years with the introgression of this event in other maize hybrids.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and susceptibility to Vip3Aa20 protein in Brazilian populations of <i>Helicoverpa armigera</i> and <i>Helicoverpa zea</i> (Lepidoptera: Noctuidae)

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Frequency of resistance to Vip3Aa20 toxin from Bacillus thuringiensis in Spodoptera frugiperda (Lepidoptera: Noctuidae) populations in Brazil

Cited by 61 publications

References 39 publications

Bacterial Vegetative Insecticidal Proteins (Vip) from Entomopathogenic Bacteria

Bacterial Vegetative Insecticidal Proteins (Vip) from Entomopathogenic Bacteria

Bacillus thuringiensis Vip3Aa Toxin Resistance in Heliothis virescens (Lepidoptera: Noctuidae)

Genetic diversity and susceptibility to Vip3Aa20 protein in Brazilian populations of <i>Helicoverpa armigera</i> and <i>Helicoverpa zea</i> (Lepidoptera: Noctuidae)

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