Frequency of Occurance of Brucellosis in Goats in Ludhiana District of Punjab State of India

Saxena, N. B. L.; Gill, James S.; Aulakh, Rabinder Singh

doi:10.9734/mrji/2017/35974

Cited by 3 publications

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“…Previous studies in Ethiopia also showed a signi cantly high seroprevalence of brucellosis in small ruminants using iELISA as compared to mRBPT [24]. Similar to the ndings of the present study, previous studies in small ruminants suggested that ELISA based test is more effective for the serological based survey of brucellosis as compared to mRBPT [16][17][18][19].…”

Section: Discussionsupporting

confidence: 87%

Seropositivity and Risk Factors of Brucella Infection in Small Ruminants that had History of Recent Abortion in the Afar Region of North-Eastern Ethiopia

Tekle

Legesse

Mamo

2021

Preprint

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Background: Brucellosis is one of the most important reproductive disease causes abortion and breeding failure in small ruminants and also causes severe systemic diseases in exposed humans. In Ethiopia, several studies of seroprevalence shows the magnitude and distribution of brucellosis both in animals and humans vary in different geographical localities. However, except few studies in Ethiopia all these serological studies was limited to RBPT and CFT, so far not supplemented with a varities of serological tests like ELISA to detect brucella infection, which is increase the likehood of detecting infected individulas and also improve the reliability of epidemiological data for appropriate control strategies. Hence, the present study was conducted in Amibara district of Afar Region, Ethiopia to detect the seropositivity and risk factor of Brucella infection in small ruminants that had history of recent abortion using mRBPT, cELISA and CFT. Materials and methods: Sera were collected from 226 animals (195 goats and 31sheep) and assessed for seropositivity of Brucella infection using modified Rose Bengal Plate Test (mRBPT), Complement Fixation Test (CFT) and competitive Enzyme Linked Immuno Sorbent Assay (cELISA).Results : In this study the over all seroprevalence was 12.0% (27 out of 226), 7.5% (17 out of 226) and 26.6% (60 out of 226) by mRBPT, CFT and cELISA, respectively. Out of 27 sera which were reactive by mRBPT, 17 (63.0%) were also reactive by (CFT). Out of the 17 sera which were reactive by CFT and mRBPT, 14 (82.4%) were reactive by cELISA. Out of the 29 sera which were non-reactive both by mRBPT and CFT, 10 (34.5%) were found to be reactive by cELISA. Out of the 226 sera which were tested both by mRBPT and cELISA, 20 (8.9%) were reactive by both tests, while 159 (70.4%) were non-reactive by both tests. The percentage of test agreement (79.2%) between mRBPT and cELISA was poor (k= 0.353). A high seropositivity for Brucella infection was significantly associated with the presence of retained placenta in the study animals (adjusted OR= 2.2, 95%CI, 1.1-4.4, P=0.030) as detected by cELISA. Conclusion: The findings of this study could suggest that brucellosis is main cause of abortion and breeding failure in small ruminants that had histry of recent abortion in the pastoral communities’ andwarrants the need for proactive measures to reduce its economic impact and risk of zoonotic transmission. This study indicates that cELISA based seroepidemiological survey increase the likehood of detecting infected individulas of brucellosis and also would be useful to provide reliable evidence for Brucella infection in small ruminants compared to mRBPT.

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Section: Discussionsupporting

confidence: 87%

Seropositivity and Risk Factors of Brucella Infection in Small Ruminants that had History of Recent Abortion in the Afar Region of North-Eastern Ethiopia

Tekle

Legesse

Mamo

2021

Preprint

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“…25 Similar to the findings of the present study, previous studies on small ruminants suggested that ELISA-based test is more effective for serologicalbased survey of brucellosis as compared to mRBPT. [16][17][18][19] In this study, the agreement between mRBPT and cELISA for the serodiagnosis of Brucella infection in small ruminants was poor. However, a previous study in Ethiopia revealed a fair agreement between mRBPT and indirect ELISA for the serodiagnosis of Brucella infection in small ruminants.…”

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confidence: 60%

“…13,14 Several studies have also suggested that ELISA is more effective for a seroepidemiological survey of brucellosis as compared to RBPT and good diagnostic results have been obtained in sheep and goats with indirect (I-) or competitive (C-) enzyme linked immunosorbent assays (ELISAs) using various antigens, but generally the ELISAs that use antigens with a high content of smooth lipopolysaccharide (sLPS) are the most useful. [15][16][17][18][19][20] In Ethiopia, several seroprevalence studies of brucellosis have shown the occurrence of brucellosis among livestock using mRBPT and CFT. [21][22][23][24] Nevertheless, few studies have used ELISA to assess the seroprevalence of brucellosis in animals.…”

Section: Introductionmentioning

confidence: 99%

Seropositivity, Comparison Between the Efficiency of Serological Tests and Risk Factors of Brucella Infection in Small Ruminants with History of Abortion in the Afar Region of North-Eastern Ethiopia

Tekle,

Legesse,

Mamo

2024

VMRR

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Purpose Brucellosis is one of the most important reproductive diseases that cause abortion and breeding failure in small ruminants in Ethiopia. Therefore, our objective was to detect the seropositivity and risk factors of Brucella infection in small ruminants with history of abortion using modified RBPT, cELISA, and CFT in the Amibara district of the Afar Region, Ethiopia. Methods Sera were collected from 226 animals (195 goats and 31ewes) and assessed for seropositivity of Brucella infection using modified RBPT, CFT, and competitive ELISA. Results The overall seroprevalence was 12.0% (27 out of 226), 7.5% (17 out of 226), and 26.5% (60 out of 226) by mRBPT, CFT, and cELISA, respectively. Out of 27 sera that were reactive by mRBPT, 17 (63.0%) were also reactive by (CFT). Out of the 17 sera that were reactive by CFT and mRBPT, 14 (82.4%) were reactive by cELISA. Out of the 29 sera that were non-reactive by both mRBPT and CFT, 10 (34.5%) were found reactive by cELISA. Out of the 226 sera that were tested by both mRBPT and cELISA, 20 (8.9%) were reactive by both tests, while 159 (70.4%) were non-reactive by both tests. The percentage of test agreement (79.2%) between mRBPT and cELISA was poor (k=0.353). High seropositivity for Brucella infection was significantly associated with the presence of retained placenta in the studied animals (adjusted OR=2.2, 95% CI, 1.1–4.4, P=0.030) as detected by cELISA. Conclusion The current study revealed that a cELISA-based seroepidemiological survey increases the likelihood of detecting individuals with brucellosis and provides reliable evidence for mRBPT. Furthermore, there was a significant association between seropositivity for Brucella infection and retained placenta. These findings emphasize the necessity for proactive measures to reduce the economic impact of brucellosis and mitigate the risk of zoonotic transmission.

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Spatial sero‐prevalence of brucellosis in small ruminants of India: Nationwide cross‐sectional study for the year 2017–2018

Shome

Kalleshamurthy

Rathore

et al. 2020

Transbound Emerg Dis

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Brucellosis in small ruminants caused mainly due to Brucella melitensis is an important zoonotic disease characterized by abortion, retained placenta, infertility, orchitis, epididymitis and rarely arthritis. Small ruminants are the main source of economy for the rural and marginally poor farmers and brucellosis is resulting in huge economic losses due to abortions and infertility and causing public health concern among the small ruminant keepers. Bovine brucellosis control programme has been implemented in India and small ruminants are left out of the programme mainly due to paucity of brucellosis status. The present cross-sectional study based on stratified random sampling was undertaken during 2017-18 to provide the nationwide brucellosis seroprevalence in small ruminants. A total of 24,056 small ruminant serum samples (sheep samples = 8,103 [male-2,440 and female-5,663] and goat samples = 15,953 [male-4,331 and female-11,622]) sourced from 27 out of 29 states and two out of seven union territories (UTs), 350 districts of total 640 districts (54.68% of the Indian districts) and from 1,462 villages out of 6,40,867 villages (43.83% of the Indian villages).The serum samples were tested by indirect ELISA and overall brucellosis apparent and true prevalence of 7.45 (95% CI: 7.13-7.79) and 3.79 (95% CI: 3.44-4.17) was recorded. Significantly higher brucellosis sero-prevalence (p < .0001) was observed in sheep (11.55%) than goats (5.37%). Similarly, brucellosis seropositivity was highly significant in females compared to males in both sheep and goats. Countrywide, greater than 5% brucellosis sero-prevalence in sheep and goats was recorded in 14 and 10 states, respectively, indicating endemicity of the disease. The study provided the latest update on nationwide spatial sero-prevalence of small ruminant brucellosis which will aid government to strengthen regular surveillance and vaccination to reduce the disease burden and public health problems in the country.

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Frequency of Occurance of Brucellosis in Goats in Ludhiana District of Punjab State of India

Cited by 3 publications

References 0 publications

Seropositivity and Risk Factors of Brucella Infection in Small Ruminants that had History of Recent Abortion in the Afar Region of North-Eastern Ethiopia

Seropositivity and Risk Factors of Brucella Infection in Small Ruminants that had History of Recent Abortion in the Afar Region of North-Eastern Ethiopia

Seropositivity, Comparison Between the Efficiency of Serological Tests and Risk Factors of Brucella Infection in Small Ruminants with History of Abortion in the Afar Region of North-Eastern Ethiopia

Spatial sero‐prevalence of brucellosis in small ruminants of India: Nationwide cross‐sectional study for the year 2017–2018

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