Frequency in the supply of Lactobacillus influence its probiotic effect for yellow tail lambari

Abstract

The aim of this research was to evaluate the changes in intestinal tract microbiota, hematological parameters and zootechnical indexes of yellowtail lambaris (Astyanax bimaculatus) fed with different probiotic supply frequencies (Lactobacillus spp.). Lambaris (1,200) were distributed in twelve boxes, divided in three treatments, 100% supply; 25% and 0% (control) of feeds supplemented with probiotic. After 87 days, fish fed with supplemented diet, regardless of frequency (100% and 25%), showed an increase in the total lactic acid bacteria count of 7.99±0.15 log CFU mL-1 and 7.04±1, 29 log UFC mL-¹ respectively, in the intestinal tract and decrease of Pseudomonas spp. in both 100% and 25% (4.09±0.84log UFC mL-1 and 4.00±1.12log UFC mL-1, respectively) in relation to the control treatment (6.74±1.04log UFC mL-1). Fish fed with 100% of diets supplemented with probiotics had a higher leukocyte count (47.7±6.7x103μL-1), lymphocytes (36.0±2.6x103μL1), monocytes (12.0±4.2x103μl-1), as well as higher mean final weight (10.60±0.89g) and weekly growth rate (0.80±0.08g.week-1) than the other treatments (25% of probiotic offered and control). Probiotic (Lactobacillus spp.) offered in 25% of feeds (or more) alters the yellowtail lambari microbiota (A. bimaculatus); however, must be offer in 100% of feeds to improve the hematology profile and productive performance.

Effects of anEnterococcus faecium‐based probiotic on growth performance and health of Pirarucu,Arapaima gigas

“…The use of lactic acid bacteria as probiotics in fish diets has been reported as an animal benefit promoter, improving growth and disease resistance (Dawood et al, ; Giri et al, ; Jatobá et al, ; Mohammadian et al, ; Mouriño et al, ). However, the present study is the first to report on the potential use of autochthonous E. faecium to modulate intestinal microbiota to improve growth as well as haematological and immunological responses for A. gigas .…”

“…In this context, reports are available indicating the use of probiotics to reduce disease outbreaks by enhancing immunological system (Dias et al, ; Soltani, Ghosh, et al, ; Soltani, Lymbery, Song, & Hosseini‐Shekarabi, ). The use of probiotics is a strategy to increase production of fish, modulating the intestinal microbiota (Mouriño et al, ; Standen et al, ), improving the immune response (Giri, Sukumaran, & Oviya, ; Jatobá Moraes, Rodrigues, Vieira, & Pereira, ; Moraes et al, ; Mouriño et al, ) and improving growth performance (Dias et al, ; Giri et al, ; Hamdan, El‐Sayed, & Mahmoud, ), during diverse stages of growth (Hamdan et al, ; Jha, Bhujel, & Anal, ; Nakandakare et al, ; Ridha & Azad, ).…”

“…Lactic acid bacteria have shown to be promising probiotics with positive results in various aquatic species including Dicentrarchus labrax (Piccolo et al, ), Oreochromis niloticus (Jatobá et al, , ), Labeo rohita (Giri et al, ) and Astyanax bimaculatus (Moraes et al, ). The positive effect is due to their capacity to colonize the gastrointestinal tract, modifying the microbiota of the intestine and inhibiting the growth of other microorganisms, and improving the immune system (Giri et al, ; Jatobá et al, ; Mouriño et al, ; Yamashita et al, ).…”

Comparative effects of autochthonous single‐strain and multi‐strain probiotics on the productive performance and disease resistance in Colossoma macropomum (Cuvier, 1818)

“…The B. cereus and multi‐strain probiotics acted on the tambaqui microbiota modulation to prevent pathogenic bacterial colonization. This probiotic effect on pathogenic bacteria is a promising feature related to the competitive exclusion of these microorganisms from the production of antimicrobials and quorum sensing (Miller & Bassler, 2001; Gatesoupe, 2008; Mouriño et al, 2017; Jatobá et al, 2018).…”

“…In addition, 100 μl aliquots of each dilution were plated in Petri dishes containing MRS and tryptone soy agar (TSA) to evaluate the growth of probiotic and potentially pathogenic bacteria respectively. These plates were incubated at 35 °C for 48 h, the cells were counted (CFU·g −1 ) (Jatobá et al, 2008) and bacterial identification was made using the MALDI‐TOF technique (Angeletti, 2017).…”