Freqüência de aglutininas anti-Leptospira interrogans em eqüídeos, em Minas Gerais, 2003 a 2004

Chiareli, Denise; Moreira, É. C.; Gutierrez, Hernan Oliver Daza; Rodrigues, Rogério Oliveira; Marcelino, Andreza Pain; Meneses, José Newton Coelho; Almeida, Virgílio

doi:10.1590/s0102-09352008000600043

Cited by 14 publications

(14 citation statements)

References 6 publications

(2 reference statements)

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“…antigens used in the battery of antigens, resulting in a 28% seropositivity rate. This frequency is within the range of seropositivity of 5.9 to 100% for horses in Brazil (Chiareli et al, 2008;Moraes et al, 2010) and reflects natural exposure to infection, since the owners in the region studied do not usually vaccinate animals against leptospirosis, which could interfere in the serological diagnosis at some point. The serovar that reacted most often was Patoc, representing 35.71% of the reactions, followed by Butembo (32.14%), Sentot (14.30%), Copenhageni (7.14%), Australis (7.14%), and Hardjo (3.57%).…”

Section: Introductionmentioning

confidence: 74%

“…However, a definitive diagnosis is possible only using laboratory techniques, such as bacterial isolation and antibody detection in serum (Braga et al, 2011). In Brazil, recent serologic surveys conducted using the microscopic agglutination test (MAT) point to a seropositivity rate ranging from 5.9 to 100% (Chiareli et al, 2008;Moraes et al, 2010). Risk factors, such as the presence of rodents in breeding facilities and food storage sites, contact with infected animals, high population density, and inadequate management practices, should be taken into account when there is suspicion of the disease in the herd (Barwick et al, 1997).…”

Section: Palavras-chavementioning

confidence: 99%

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Epidemiological characterization of leptospirosis in horses in the state of Pernambuco, northeastern Brazil

et al. 2016

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An epidemiological survey aiming to determine the occurrence of anti-Leptospira antibodies and to identify risk factors was carried out in horses of the Pajeú microregion, state of Pernambuco. Sera from 100 horses from 38 properties in four counties in the region were examined by the microscopic agglutination test (MAT), using 24 Leptospira spp. serovars as antigens. Of the 100 samples tested, 28 (28%) were positive. Most prevalent serovars were Patoc (35.71%), Butembo (32.14%) and Sentot (14.30%). Supply of pasture as forage (odds ratio =7.80; 95% confidence interval - 95%CI 1.71-35.50; p=0.008) was identify as risk factor. It is suggested the need for studies on agent isolation and characterization of its pathogenicity, as well as it is recommended the rodent control in deposits of feed and environment.

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Section: Introductionmentioning

confidence: 74%

Section: Palavras-chavementioning

confidence: 99%

Epidemiological characterization of leptospirosis in horses in the state of Pernambuco, northeastern Brazil

et al. 2016

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“…O presente trabalho desenvolveu uma bacterina monovalente utilizando a sorovariedade Hardjo amostra Norma, pelo fato desta ser um isolado brasileiro e também por ser a sorovariedade mais prevalente no país (Moreira 1994, Hermann et al 2004, Araújo et al 2005, Lage et al 2007, Chiareli et al 2008), e comparou-a com as bacterinas existentes no mercado brasileiro.…”

Section: Discussionunclassified

Comparação entre a imunidade induzida em bovinos vacinados com bacterinas polivalentes comerciais e uma monovalente experimental

et al. 2011

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O presente estudo avaliou a indução da produção de anticorpos contra Leptospira spp.por dez bacterinas, sendo nove polivalentes e uma monovalente experimental para a sorovariedade Hardjo amostra Norma. A concentração celular foi controlada e utilizou-se adjuvante de emulsão óleo em água. Um ensaio imunoenzimático (ELISA) indireto foi desenvolvido utilizando-se conjugado anti-IgG total para mensurar os níveis de anticorpos da classe IgG conferido pelas bacterinas utilizando três amostras diferentes: Hardjoprajitino, Norma e Hardjo-bovis. Paralelamente foi utilizado também o Teste de Soroaglutinação Microscópica (SAM) para mensurar os níveis de anticorpos contra as mesmas amostras. Encontraram-se títulos variáveis entre as bacterinas de acordo com o teste ELISA. Os títulos no SAM foram de pouca intensidade e de curta duração indicando a necessidade de controle celular para uma posterior padronização destes produtos. Com base nos resultados encontrados no presente estudo, a bacterina monovalente foi a que apresentou melhor desempenho.

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“…The disease has a worldwide distribution, but some serovars are found more frequently in certain geographical areas or in certain animal species (Oliveira and Pires Neto, 2004). The main clinical manifestations in livestock involve reproductive problems such as infertility, abortion in late pregnancy or birth of debilitated animals (Chiareli, 2007). In equids, leptospirosis manifests itself by recurrent uveitis as well as abortion and other reproductive disorders.…”

Section: Introductionmentioning

confidence: 99%

Spatial characterization of Leptospira spp. infection in equids from the Brejo Paraibano micro-region in Brazil

et al. 2014

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Abstract. The present study, the first to spatially characterize Leptospira spp. infection among equids in the Brejo Paraibano micro-region of the Paraiba state in the northeast of Brazil, investigated 257 animals in 26 farms properties. Serum samples from 204 horses, 46 mules and seven donkeys were serologically diagnosed using the microscopic agglutination test (MAT). The distribution of Leptospira spp. was studied by employing specific antigens from 24 different Leptospira serovars. All farms were georeferenced and their distribution visualised on a map of the Brejo Paraibano micro-region. In addition, rainfall data were obtained from the same year, in which the sampling was performed. Among the 20 farms found to harbour animals with leptospirosis, 14 (70%) exhibited low prevalence, five (25%) medium prevalence and one (5%), high prevalence. Certain areas had a higher density of infected farms and required intervention to control the infection. Many serovars were widely distributed, while others were more common in particular areas. There was no significant association between the prevalence of Leptospira spp. infection and rainfall.

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Freqüência de aglutininas anti-Leptospira interrogans em eqüídeos, em Minas Gerais, 2003 a 2004

Cited by 14 publications

References 6 publications

Epidemiological characterization of leptospirosis in horses in the state of Pernambuco, northeastern Brazil

Epidemiological characterization of leptospirosis in horses in the state of Pernambuco, northeastern Brazil

Comparação entre a imunidade induzida em bovinos vacinados com bacterinas polivalentes comerciais e uma monovalente experimental

Spatial characterization of Leptospira spp. infection in equids from the Brejo Paraibano micro-region in Brazil

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