Frame‐Guided Assembly of Amphiphiles

Abstract

Frame-guided assembly, a recently discovered strategy for amphiphilic assembly, is discussed as a strategy for constructing vesicle assemblies with programmed geometries and dimensions under identical surrounding conditions. The strategy is inspired by the cytoskeletal-membrane protein-lipid bilayer structure and shows great potential in the understanding and controlling of the amphiphilic assembly process. Both the principles and basic requirements are discussed, along with recently reported examples. The prospects and potential investigations of frame-guided assembly are also proposed.

Fold 2D Woven DNA Origami to Origami+ Structures

“…Through AFM, as shown Figure c, after the addition of SDS, the unfolded origami was observed with the height increased to ≈4 nm. This is consistent with FGA strategy, according to which, the anchored cholesterol groups can guide the assembly of SDS on the origami . After the removal of SDS, the DNA sheet folded to the origami + again, whose structure is similar to those achieved from the T all through pathway A.…”

“…According to a recently reported frame guided assembly (FGA) strategy, the anchored cholesterol groups can also be used to guide other amphiphiles to assemble on the origami as reported previously . The DNA origami + structure was maintained by the hydrophobic interactions, so it can be anticipated that by adding strong detergent such as sodium dodecyl sulfate (SDS), the origami + can open its folded structure.…”

Amphiphilic DNA Organic Hybrids: Functional Materials in Nanoscience and Potential Application in Biomedicine

“…Besides the self-assembly of pristine DNA–lipid conjugates, it can be mixed or covalent with other agents such as DNA nanostructures [30] or proteins [31] to assemble into hetero-nanostructures. In 2015, Liu and colleagues utilized the amphiphilic DNA-cholesterol conjugates as the leading hydrophobic groups to induce the guided assembly of amphiphiles sodium dodecyl sulfate (SDS) on the gold nanoparticles, with results of the formation of hetero-vesicles with different sizes and shapes [32], as shown in Figure 2.…”

A Modularly Designable Vesicle for Sequentially Multiple Loading

“…However, coloading hydrophilic–hydrophobic small molecules, oligonucleotides and peptides in the same size and shape‐controlled vesicle through a stepwise manner with high efficiency is still challenging. In this paper, we report a modularly designable vesicle for sequentially multiple loading based on frame‐guided assembly (FGA), which is believed to be an outstanding platform for drug delivery in the future.…”

“…Following the principle of FGA, vesicles composed of poly(propylene oxide)‐ b ‐DNA (PPO‐ b ‐DNA) are prepared in a stepwise manner[19c] and are divided into several domains for different cargos loading: as shown in Figure . Gold nanoparticle (GNP) is modified with longer and shorter thiolated DNA in a given ratio.…”