Fractal analysis and histomolecular phenotyping provides insights into extracellular matrix remodeling in the developing bovine fetal ovary

Franchi, F; Hernandes, Mariana Pelicho; Ferreira, Amanda Leticia Coalho; Lima, Victor Augusto Vieira de; Mendes, Leonardo de Oliveira; Aquino, Ariana Musa de; Scarano, Wellerson Rodrigo; Castilho, A. C. S.

doi:10.1016/j.bbrc.2020.01.025

Cited by 9 publications

(4 citation statements)

References 12 publications

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“…As previously described, ECM molecules have auto symmetry. 24,25,39 This standard characteristic of the ECM can be used to calculate ECM deposition and organization in normal and failing hearts. 40,41 Using a similar approach, we report a quantitative description of auto symmetry by calculating the Hausdorff's (fractal) dimension, demonstrating that our dECM powder particles are in the range of cadaveric young-to-middle age hearts, 42 preserving their native architectural structure.…”

Section: Discussionmentioning

confidence: 99%

Cues from human atrial extracellular matrix enrich the atrial differentiation of human induced pluripotent stem cell-derived cardiomyocytes

Mesquita¹,

Morrissey²,

Lee³

et al. 2021

Biomater. Sci.

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Section: Discussionmentioning

confidence: 99%

Cues from human atrial extracellular matrix enrich the atrial differentiation of human induced pluripotent stem cell-derived cardiomyocytes

Mesquita¹,

Morrissey²,

Lee³

et al. 2021

Biomater. Sci.

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“…More importantly, the morphological characteristics of the ovary are closely related to the quantity of follicles, exerting an indirect influence on female animal fertility. Enhancing our knowledge of ovarian characteristics is crucial for advancing animal fertility, which is a pivotal factor in increasing the profitability of the animal breeding industry [ 29 ]. Scientists have explored the association between ovarian traits and polymorphic mutations.…”

Section: Discussionmentioning

confidence: 99%

Exploration of the Polymorphism Distribution of Bovine HMGA2 Gene in Worldwide Breeds and Its Associations with Ovarian Traits

Shen,

Zhu,

Yang

et al. 2024

Animals

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The high-mobility group AT-hook 2(HMGA2) gene has been widely studied in the context of cancer and animal growth. However, recently, several studies have uncovered its critical role in cell proliferation. A genome-wide association study (GWAS) further suggests that the HMGA2 gene is a candidate gene in fertility, indicating its connection not only to growth traits but also to reproduction, specifically ovarian traits. Thus, this study aimed to analyze the distribution of the HMGA2 gene in 54 bovine breeds worldwide, identify important short fragment variants (indels), and investigate the relationship between HMGA2 and ovarian development. The dataset included genotypic information from a bovine population of 634 individuals (n = 634). After genotyping and analyzing four selected loci, we found that one out of four loci, rs133750033 (P4-D22-bp), was polymorphic. Our results also reveal that this indel of HMGA2 is significantly associated with certain ovarian traits (p < 0.05). Specifically, it has connection with ovarian length (p = 0.004) and ovarian height (p = 0.026) during diestrus. Additionally, we discovered a higher expression of the HMGA2 gene in Asian cattle breeds. In summary, this study suggests that HMGA2 has the potential to serve as an animal fertility testing marker gene. Moreover, these findings contribute to a more promising outlook for the bovine industry.

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“…Previous studies have found that abnormal expression of the COL1A1 gene may affect the structure and function of normal ovaries 28 , and its expression level is closely related to several ovulation disorders, such as polycystic ovary syndrome (PCOS) 29 . In addition, the abnormal expression of the COL1A1 gene also re ects morphological changes in the structure and organization of the bovine foetal ovary, indicating that the COL1A1 gene plays a role in regulating the development of the bovine foetal ovary and the remodelling of the embryonic mesenchyme 30 . In conclusion, we speculate that the high expression of COL1A1 protein in ovarian tissue of polytocous goat goats may promote the association between growth factors, cell adhesion molecules, regulatory factors and connexins, thereby strengthening the communication between cells and jointly maintaining ovarian tissue structure and follicle development by providing structural support for cells.…”

Section: Discussionmentioning

confidence: 99%

COL1A1 may be associated with lambing traits in goats by regulating the biological function of ovarian granulosa cells via the PI3K/AKT/mTOR signalling pathway

Zhou

Zhao

Chen

et al. 2023

Preprint

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Collagen type I alpha 1 chain (COL1A1), an important component of type I collagen protein, plays a role in regulating follicle development and ovarian cell proliferation, which is closely related to animal reproductive traits. The aim of this study was to explore the relationship between the expression of COL1A1 and lambing traits of Guizhou black goats (Capra hircus), and regulatory mechanisms in follicle development. Results showed that the protein expression level of COL1A1 in ovarian tissues of the polytocous group were higher than those of the monotocous group, and COL1A1 was expressed in all follicles and ovarian cells. Upregulation of COL1A1 significantly promoted cell proliferation, migration and the development of cells from G1 to G2/M phase, reduced intracellular ROS levels. Additionally, COL1A1 overexpression resulted in significantly increased expression of proliferating cell nuclear antigen (PCNA) and the ratios of p-PI3K (phosphorylation-phosphatidylinositol 3-kinase)/PI3K, p-AKT (phosphorylation-protein kinase B)/AKT and p-mTOR (phosphorylation-mammalian target of rapamycin) /mTOR, and decreased expression of BCL2-Associated X (BAX), caspase-3 and caspase-9. The reverse result, however, was seen when COL1A1 expression was downregulated in granulosa cells. Taken together, COL1A1 was widely and highly expressed in ovarian tissues of polytocous goats, and it may participate in regulations of lambing traits by regulating the proliferation and migration of granulosa cells by activating the PI3K/AKT/mTOR signalling pathway and reducing ROS production in granulosa cells to facilitate the development and maturation of follicle.

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Fractal analysis and histomolecular phenotyping provides insights into extracellular matrix remodeling in the developing bovine fetal ovary

Cited by 9 publications

References 12 publications

Cues from human atrial extracellular matrix enrich the atrial differentiation of human induced pluripotent stem cell-derived cardiomyocytes

Cues from human atrial extracellular matrix enrich the atrial differentiation of human induced pluripotent stem cell-derived cardiomyocytes

Exploration of the Polymorphism Distribution of Bovine HMGA2 Gene in Worldwide Breeds and Its Associations with Ovarian Traits

COL1A1 may be associated with lambing traits in goats by regulating the biological function of ovarian granulosa cells via the PI3K/AKT/mTOR signalling pathway

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