FOXO3a-driven miRNA signatures suppresses VEGF-A/NRP1 signaling and breast cancer metastasis

Song, Ying; Zeng, Shanshan; Zheng, Guopei; Chen, Danyang; Li, Pan; Yang, Minqiang; Luo, Kai; Yin, Jun; Gu, Yue; Zhang, Zhijie; Jia, Xiaoting; Qiu, Ni; He, Zhiwei; Li, Hongsheng; Liu, Hao

doi:10.1038/s41388-020-01562-y

Cited by 44 publications

(31 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…VEGF-A can increase the permeability of and promote the formation of new blood vessels. 35 A recent study confirms that VEGF-A promotes Snail1 nuclear localization to drive the EMT by combining with the receptor in PCa. 36 VEGF-D plays a role in the formation of new blood vessels and lymphatic vessels in cancer tissue.…”

Section: Discussionmentioning

confidence: 85%

miR-593-3p Promotes Proliferation and Invasion in Prostate Cancer Cells by Targeting ADIPOR1

Huang

Peng

Sun

et al. 2021

OTT

View full text Add to dashboard Cite

Background Accumulating evidence has indicated that dysregulation of microRNAs (miRNAs) contributes to the tumorigenesis of prostate cancer (PCa). Nevertheless, the role of miR-593-3p in the development of PCa remains unclear. The objective of this study was to investigate the role and mechanisms of miR-593-3p in PCa cells. Methods RT-PCR was used to detect the expression levels of miR-593-3p. CCK-8, colony formation, spheroid formation and transwell assays were performed to examine the proliferation, migration and invasion of C4-2, DU145 and RWPE-1 cells. And then, transcriptome sequencing, dual-luciferase reporter assay and Western blot were taken to identify the target gene and downstream mechanisms of miR-593-3p. Results Here, we found that miR-593-3p promoted PCa cell proliferation, colony formation, spheroid formation, migration and invasion. Further mechanistic studies revealed that miR-593-3p possessed binding sites of ADIPOR1 3ʹ-UTR and played an important role in 5ʹ-AMP-activated protein kinase (AMPK) signaling pathway and epithelial–mesenchymal transition (EMT) process. In addition, the transfection of si-ADIPOR1 also enhanced the PCa cell proliferation and invasion. Conclusion Our study provides an empirical investigation of miR-593-3p, which exerts oncogenic function through targeting ADIPOR1 in PCa cells.

show abstract

Section: Discussionmentioning

confidence: 85%

miR-593-3p Promotes Proliferation and Invasion in Prostate Cancer Cells by Targeting ADIPOR1

Huang

Peng

Sun

et al. 2021

OTT

View full text Add to dashboard Cite

show abstract

“…Bioinformatic analysis using the Enhancer database revealed that the Snail promotor contains four consensus binding sites for FOXO3, raising the possibility that FOXO3 might directly regulate Snail expression through its ability to act as a transcriptional repressor. However, it has also been reported that FOXO3 can inhibit gene expression of Y-Box Binding protein-1 (YB-1), a protein that activates cap-independent translation of Snail mRNA [ 69 ], and/or induce mir-29, 30, and 34 transcription to reduce Snail mRNA levels [ 70 , 71 , 72 ], suggesting the potential for indirect post-transcriptional regulation. Therefore, the regulation of Snail expression by FOXO3 is complex, and further studies are required to determine the exact mechanisms involved in RA FLS.…”

Section: Discussionmentioning

confidence: 99%

14-3-3η Promotes Invadosome Formation via the FOXO3–Snail Axis in Rheumatoid Arthritis Fibroblast-like Synoviocytes

Kadiri

Charbonneau

Lalanne

et al. 2021

IJMS

View full text Add to dashboard Cite

Erosive destruction of joint structures is a critical event in the progression of rheumatoid arthritis (RA), in which fibroblast-like synoviocytes (FLS) are the primary effectors. We previously reported that the ability of RA FLS to degrade extracellular matrix (ECM) components depends on the formation of actin-rich membrane protrusions, called invadosomes, through processes that remain elusive. 14-3-3η belongs to a family of scaffolding proteins involved in a wide range of cellular functions, and its expression is closely related to joint damage and disease activity in RA patients. In this study, we sought to assess the role of 14-3-3η in joint damage by examining its contribution to the invadosome formation phenotype of FLS. Using human primary FLS, we show that 14-3-3η expression is closely associated with their ability to form invadosomes. Furthermore, knockdown of 14-3-3η using shRNAs decreases the level of invadosome formation in RA FLS, whereas addition of the recombinant protein to FLS from healthy individuals promotes their formation. Mechanistic studies suggest that 14-3-3η regulates invadosome formation by increasing Snail expression, a mechanism that involves nuclear exclusion of the transcription repressor FOXO3. Our results implicate the 14-3-3η–FOXO3–Snail axis in promoting the aggressive ECM-degrading phenotype of RA FLS, and suggest a role for this scaffolding protein in cartilage degradation.

show abstract

“…Also, a miR-338-3p predicted target, VAPB, is recently suggested as a diagnostic biomarker in ALS that complicated in ER-associated aggregates [59]. More recently, Song et al reported FoxO3a induces miR-338-3p expression and may play a critical role in decreasing cell survival through directly suppressing the expression of NRP1 [60].…”

Section: Discussionmentioning

confidence: 99%

Circulating miRNA biomarkers for sporadic Amyotrophic Lateral Sclerosis: a systematic meta-analysis and empirical validation

Daneshafrooz¹,

Joghataei²,

Mehdizadeh³

et al. 2021

Preprint

View full text Add to dashboard Cite

Amyotrophic lateral sclerosis (ALS) is a lethal neurodegenerative disease that occurs as sporadic (sALS) in most cases. The disease is not curable, and its pathogenesis is not understood as yet. Given the intricacy of underlying molecular interactions and heterogeneity of ALS, the discovery of molecules contributing to disease onset and progression will open the way for advancement in prevention and therapeutic intervention. Here we conducted a meta-analysis of 12 circulating miRNA profiling studies using the robust rank aggregation (RRA) method, followed by enrichment analysis. We identified miR-451a and let-7f-5p as meta-signature miRNAs whose targets are involved in critical pathogenic pathways underlying ALS, including FoxO, MAPK, and apoptosis. A systematic review of 7 gene profiling studies elucidated that 241 genes upregulated in sALS circulation with concomitant being targets of the meta-signature miRNAs. Protein-protein interaction network analysis for selected targets revealed the main subcluster is involved in multiple cascades, which eventually leads to apoptosis. Besides, evaluation of relative expression of miRNAs by TaqMan RT-qPCR verified let-7f-5p is significantly downregulated in the plasma of patients. Furthermore, we verified the relative expression of two top-ranked upregulated miRNAs and found miR-338-3p significantly upregulated in ALS patients. Receiver operating characteristic (ROC) analysis indicated that let-7f-5p and miR-338-3p are useful plasms biomarkers for diagnosis and a potential therapeutic target for ALS disease.

show abstract

FOXO3a-driven miRNA signatures suppresses VEGF-A/NRP1 signaling and breast cancer metastasis

Cited by 44 publications

References 47 publications

miR-593-3p Promotes Proliferation and Invasion in Prostate Cancer Cells by Targeting ADIPOR1

miR-593-3p Promotes Proliferation and Invasion in Prostate Cancer Cells by Targeting ADIPOR1

14-3-3η Promotes Invadosome Formation via the FOXO3–Snail Axis in Rheumatoid Arthritis Fibroblast-like Synoviocytes

Circulating miRNA biomarkers for sporadic Amyotrophic Lateral Sclerosis: a systematic meta-analysis and empirical validation

Contact Info

Product

Resources

About