Forty Years of Homograft Surgery

Yankah, A. C.

doi:10.1177/021849230201000201

Cited by 5 publications

(3 citation statements)

References 16 publications

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“…Decellulized AHV could supposedly be seeded with genetically manipulated and/or cultured autologous endothelial cells and fibroblasts. This might solve the major problem encountered in young patients, who are immunologically active, have growth potential and need durable grafts (Yankah 2002).…”

Section: Discussionmentioning

confidence: 99%

Allograft semilunar cardiac valves processing and cryopreservation – morphology in scanning electron microscope

Krs

Burkert

Slížová

et al. 2006

Cell Tissue Banking

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Section: Discussionmentioning

confidence: 99%

Allograft semilunar cardiac valves processing and cryopreservation – morphology in scanning electron microscope

Krs

Burkert

Slížová

et al. 2006

Cell Tissue Banking

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“…One method of treatment of inborn and acquired valve diseases is replacement of damaged valves by auto-, allo-, or xenograft (14). Retrospective and morphological studies get evidence that integrity of endothelial covering together with viability of deeper valve structures are decisive for further fate of the transplanted cardiac valve (3,4,7,11).…”

Section: Introductionmentioning

confidence: 99%

Impact of Processing on Surface Structure of Human Cardiac Valve Allografts

Krs

Slížová²,

Burkert³

et al. 2004

Acta Med. (Hradec Kralove, Czech Repub.)

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Methods of processing and cryopreservation are believed to be the most important factors of long term clinical performance of biological heart valve prostheses. That is why we decided to cooperate in evaluating the impact of current AHV (allograft heartvalve) bank protocol on valve tissue morphology. AHV harvested from „heart-beating“ cadaveric donors, considered as a fresh tissue, were compared with valve samples from non-heart beating donors, samples stored in saline, samples treated with antibiotic solution, and finally with cryopreserved valves, stored in liquid nitrogen for months. All samples were dissected, dried with hexamethyldisilazane (HMDS) method, gold-coated, studied and photographed in scanning electron microscope Tesla BS 301. Different superficial patterns were found on ventricular and vascular surfaces of „fresh“ semilunar valves. We were able to detect early changes of endothelium after harvesting, denudation of endothelial covering during preservation with and without freezing. Our alternative method of drying samples by HMDS method proved to be suitable for thin membranes of human semilunar valves. Scanning electron microscopy seems to be helpful for morphological control of processing, cryopreservation and liquid nitrogen storage of AHV. We believe that further confrontation of morphological investigation with other methods helps us to develop more suitable protocol of handling AHV in heart valve banking.

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“…Decellularized AHV could supposedly be seeded with genetically manipulated and / or cultured autologous endothelial cells and fibroblasts [5,6,11,17,20,32]. Hypothetically, this might solve the major problem encountered in young patients, who are immunologically active, have growth potential and need durable grafts [41]. Our SEM method could be useful and cost-effective in that field of research [16,35].…”

mentioning

confidence: 99%

Cryopreserved Semilunar Heart Valve Allografts: Leaflet Surface Damage in Scanning Electron Microscopy

Burkert¹,

Krs²,

Vojáček³

et al. 2008

Zentralbl Chir

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SEM (using HMDS drying) together with other methods may be helpful for the morphological control of processing, cryopreservation and liquid nitrogen storage of AHV. Severe AHV leaflet endothelial destruction was proven on AHV grafts. These changes arose already in the initial steps of tissue processing, just after the donor heart harvesting and then at the time of antibiotic valve graft treatment. These results are considered as the starting point for the development of a better preservation protocol.

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Forty Years of Homograft Surgery

Abstract: EDITORIALThis college must play its part in keeping alive the historic tradition of the medical profession, and must ever foster those high standards of professional behavior which distinguish a profession from a trade. -Winston S Churchill

Cited by 5 publications

References 16 publications

Allograft semilunar cardiac valves processing and cryopreservation – morphology in scanning electron microscope

Allograft semilunar cardiac valves processing and cryopreservation – morphology in scanning electron microscope

Impact of Processing on Surface Structure of Human Cardiac Valve Allografts

Cryopreserved Semilunar Heart Valve Allografts: Leaflet Surface Damage in Scanning Electron Microscopy

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